Semi-nested RT-PCR enables sensitive and high-throughput detection of SARS-CoV-2 based on melting analysis.

Autor: Thi Nguyen NA; Department of Genomics & Cytogenetics, Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Viet Nam., Thi Bui H; Department of Genomics & Cytogenetics, Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Viet Nam., Thi-Huong Pham Q; Department of Genomics & Cytogenetics, Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Viet Nam., Thi Thao Hoang L; Department of Genomics & Cytogenetics, Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Viet Nam., Xuan Ta H; Top Data Science Ltd, Helsinki, Finland., Heikkinen T; Top Data Science Ltd, Helsinki, Finland., Van Le D; National Hospital of Tropical Diseases, 78 Giai Phong, Dong Da, Hanoi, Viet Nam., Dinh Van T; National Hospital of Tropical Diseases, 78 Giai Phong, Dong Da, Hanoi, Viet Nam., Quoc Ngo N; Phusa Biochem Biochemistry Ltd, Can Tho, Viet Nam., Thi Hong Huynh P; Phusa Biochem Biochemistry Ltd, Can Tho, Viet Nam., Thi Huyen Tran T; Department of Molecular Biology, 108 Hospital, Hanoi, Viet Nam; Vietnamese - German Centre of Excellence in Medical Research, 108 Hospital, Hanoi, Viet Nam., Quoc Phan H; Department of Molecular Biology, 108 Hospital, Hanoi, Viet Nam., Van Hoang L; Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Vietnam., van Doorn HR; Oxford University Clinical Research Unit, Hanoi, Viet Nam; Nuffield Department of Clinical Medicine, University of Oxford, Oxford, UK., Thi Ngoc Nguyen D; Oxford University Clinical Research Unit, Hanoi, Viet Nam., Thi Nguyen T; Oxford University Clinical Research Unit, Hanoi, Viet Nam., Sy Vo N; Department of Computational Biomedicine, Vingroup Big Data Institute, Hanoi, Viet Nam., Viet Vo C; Vietnamese- Russian Tropical Center, Hanoi, Viet Nam., Khac Trinh S; Vietnamese- Russian Tropical Center, Hanoi, Viet Nam., The Pham T; Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Vietnam., Duc Le Q; Department of Systems Engineering and Computer Network, National University of Civil Engineering, Hanoi, Viet Nam., Van Le P; College of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Viet Nam., Thai Nguyen S; 103 Military Hospital, Vietnam Military Medical University, Hanoi, Viet Nam., Thi Tran L; Department of Genomics & Cytogenetics, Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Viet Nam., Dinh Vu T; Department of Genomics & Cytogenetics, Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Viet Nam., Vu Nguyen QA; Department of Genomics & Cytogenetics, Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Viet Nam., Thi Trieu N; Department of Genomics & Cytogenetics, Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Viet Nam., Thi Le T; Department of Genomics & Cytogenetics, Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Viet Nam., Dinh Nguyen U; Department of Genomics & Cytogenetics, Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Viet Nam., Steman J; Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden; Minerva Foundation Institute for Medical Research, Helsinki, Finland., Huu Ho T; Department of Genomics & Cytogenetics, Institute of Biomedicine and Pharmacy (IBP), Vietnam Military Medical University, Hanoi, Viet Nam; 103 Military Hospital, Vietnam Military Medical University, Hanoi, Viet Nam. Electronic address: hohuutho@vmmu.edu.vn.

Publikováno v: Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2022 Jun 01; Vol. 531, pp. 309-317. Date of Electronic Publication: 2022 Apr 29.