Long-Term Adult Feline Liver Organoid Cultures for Disease Modeling of Hepatic Steatosis

Autor: Kruitwagen, Hedwig S., Oosterhoff, Loes A., Vernooij, Ingrid G W H, Schrall, Ingrid M., van Wolferen, Monique E., Bannink, Farah, Roesch, Camille, van Uden, Lisa, Molenaar, Martijn R., Helms, J. Bernd, Grinwis, Guy C.M., Verstegen, Monique M A, van der Laan, Luc J W, Huch, Meritxell, Geijsen, Niels, Vries, Robert R G, Clevers, Hans, Rothuizen, Jan, Schotanus, Baukje A., Penning, Louis C., Spee, Bart, dB&C FR-RMSC FR, dCSCA RMSC-1, dPB CR, dCSCA AVR, Onderzoek, Biochemisch laboratorium, LS Pathologie, Sub Biochemie Algemeen, Dep Biochemie en Celbiologie, LS Veterinaire biochemie, Veterinair Pathologisch Diagnostisch Cnt, PB AVM, Applied Veterinary Research, Dep Pathobiologie, Faculteit Diergeneeskunde, LS Interne geneeskunde

Publikováno v: Stem Cell Reports, Vol 8, Iss 4, Pp 822-830 (2017)
Stem Cell Reports, 8(4), 822-830. Cell Press
Stem Cell Reports, 8(4), 822. Cell Press
Stem Cell Reports
Stem Cell Reports [E], 8(4), 822. Cell Press

Summary Hepatic steatosis is a highly prevalent liver disease, yet research is hampered by the lack of tractable cellular and animal models. Steatosis also occurs in cats, where it can cause severe hepatic failure. Previous studies demonstrate the po

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d39c837bc75176cbf7a2f6a92d149ded
https://doi.org/10.1016/j.stemcr.2017.02.015

Long-term culture of genome-stable bipotent stem cells from adult human liver

Autor: Huch, Meritxell, Gehart, Helmuth, van Boxtel, Ruben, Hamer, Karien, Blokzijl, Francis, Verstegen, Monique M. A., Ellis, Ewa, van Wenum, Martien, Fuchs, Sabine A., de Ligt, Joep, van de Wetering, Marc, Sasaki, Nobuo, Boers, Susanne J., Kemperman, Hans, de Jonge, Jeroen, Ijzermans, Jan N. M., Nieuwenhuis, Edward E. S., Hoekstra, Ruurdtje, Strom, Stephen, Vries, Robert R. G., van der Laan, Luc J. W., Cuppen, Edwin, Clevers, Hans

Publikováno v: Cell, 160(1-2), 299-312. Elsevier B.V.
CELL
Cell, 160(1-2), 299-312. Cell Press
Cell, 160(1-2), 299. Cell Press

SummaryDespite the enormous replication potential of the human liver, there are currently no culture systems available that sustain hepatocyte replication and/or function in vitro. We have shown previously that single mouse Lgr5+ liver stem cells can

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::536b0185ba6bbdeca869790f23ac4059
https://www.repository.cam.ac.uk/handle/1810/246736

Disease Modeling and Gene Therapy of Copper Storage Disease in Canine Hepatic Organoids

Autor: Nantasanti, Sathidpak, Spee, Bart, Kruitwagen, Hedwig S, Chen, Chen, Geijsen, Niels, Oosterhoff, Loes A, van Wolferen, Monique E, Pelaez, Nicolas, Fieten, Hille, Wubbolts, Richard W, Grinwis, Guy C, Chan, Jefferson, Huch, Meritxell, Vries, Robert R G, Clevers, Hans, de Bruin, Alain, Rothuizen, Jan, Penning, Louis C, Schotanus, Baukje A, CSCA TR1, B&C BRC-SIB, PB TR, dPB CR, Onderzoek, Biochemisch laboratorium, LS Interne geneeskunde, LS Celbiologie-Algemeen, Sub Center for Cell Imaging, Veterinair Pathologisch Diagnostisch Cnt, Dep Pathobiologie, Pathologie, LS Pathobiologie, Sub Neuro/Tandheelkunde

Publikováno v: Stem Cell Reports. Cell Press
Stem Cell Reports [E], 5(5), 895. Cell Press
Stem Cell Reports, Vol 5, Iss 5, Pp 895-907 (2015)
Stem Cell Reports
Stem Cell Reports, 5(5), 895-907. CELL PRESS

Summary The recent development of 3D-liver stem cell cultures (hepatic organoids) opens up new avenues for gene and/or stem cell therapy to treat liver disease. To test safety and efficacy, a relevant large animal model is essential but not yet estab

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2ea3a6975a028efa43e1f5b6771ddbb

Long-term culture of genome-stable bipotent stem cells from adult human liver.

Autor: Huch M; Hubrecht Institute-KNAW, University Medical Centre Utrecht, CancerGenomics.nl, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands. Electronic address: m.huch@gurdon.cam.ac.uk., Gehart H; Hubrecht Institute-KNAW, University Medical Centre Utrecht, CancerGenomics.nl, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., van Boxtel R; Hubrecht Institute-KNAW, University Medical Centre Utrecht, CancerGenomics.nl, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Hamer K; Hubrecht Institute-KNAW, University Medical Centre Utrecht, CancerGenomics.nl, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Blokzijl F; Hubrecht Institute-KNAW, University Medical Centre Utrecht, CancerGenomics.nl, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Verstegen MM; Department of Surgery, Erasmus MC-University Medical Center, Postbus 2040, 3000 CA Rotterdam, the Netherlands., Ellis E; Unit for Transplantation Surgery, Department of CLINTEC, Karolinska Institute, Karolinska University Hospital Huddinge, Hälsovägen, Flemingsberg, SE-141 86 Stockholm, Sweden., van Wenum M; Surgical Laboratory, Tytgat Institute for Liver and Intestinal Research, Academic Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, the Netherlands., Fuchs SA; Division of Pediatric Gastroenterology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Lundlaan 6, 3584 EA Utrecht, the Netherlands., de Ligt J; Hubrecht Institute-KNAW, University Medical Centre Utrecht, CancerGenomics.nl, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., van de Wetering M; Hubrecht Institute-KNAW, University Medical Centre Utrecht, CancerGenomics.nl, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands; Hubrecht Organoid Technology (HUB), Uppsalalaan 8, 3584CT, Utrecht, the Netherlands., Sasaki N; Hubrecht Institute-KNAW, University Medical Centre Utrecht, CancerGenomics.nl, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Boers SJ; Division of Pediatric Gastroenterology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Lundlaan 6, 3584 EA Utrecht, the Netherlands., Kemperman H; Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, Lundlaan 6, 3584 EA Utrecht, the Netherlands., de Jonge J; Department of Surgery, Erasmus MC-University Medical Center, Postbus 2040, 3000 CA Rotterdam, the Netherlands., Ijzermans JN; Department of Surgery, Erasmus MC-University Medical Center, Postbus 2040, 3000 CA Rotterdam, the Netherlands., Nieuwenhuis EE; Division of Pediatric Gastroenterology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Lundlaan 6, 3584 EA Utrecht, the Netherlands., Hoekstra R; Surgical Laboratory, Tytgat Institute for Liver and Intestinal Research, Academic Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, the Netherlands., Strom S; Division of Pathology, Department of Laboratory Medicine, Karolinska Institute, Alfred Nobels Alle 8, F 56 141-86 Stockholm, Sweden., Vries RR; Hubrecht Institute-KNAW, University Medical Centre Utrecht, CancerGenomics.nl, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands; Hubrecht Organoid Technology (HUB), Uppsalalaan 8, 3584CT, Utrecht, the Netherlands., van der Laan LJ; Department of Surgery, Erasmus MC-University Medical Center, Postbus 2040, 3000 CA Rotterdam, the Netherlands., Cuppen E; Hubrecht Institute-KNAW, University Medical Centre Utrecht, CancerGenomics.nl, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands., Clevers H; Hubrecht Institute-KNAW, University Medical Centre Utrecht, CancerGenomics.nl, Uppsalalaan 8, 3584 CT Utrecht, the Netherlands. Electronic address: h.clevers@hubrecht.eu.

Publikováno v: Cell [Cell] 2015 Jan 15; Vol. 160 (1-2), pp. 299-312. Date of Electronic Publication: 2014 Dec 18.