Zobrazeno 1 - 10
of 101
pro vyhledávání: '"Volker Kasche"'
Publikováno v:
FEBS Letters. 579:5069-5073
Several factors at transcriptional, post-transcriptional or post-translational level determine the fate of a target protein and can severely restrict its yield. Here, we focus on the post-transcriptional regulation of the biosynthesis of the periplas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9225a5295784de49e890cc65dbc8bb9
https://doi.org/10.1016/j.febslet.2005.08.018
https://doi.org/10.1016/j.febslet.2005.08.018
Publikováno v:
European Journal of Biochemistry. 270:4721-4728
Penicillin amidase from Alcaligenes faecalis is a recently identified N-terminal nucleophile hydrolase, which possesses the highest specificity constant (kcat/Km) for the hydrolysis of benzylpenicillin compared with penicillin amidases from other sou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d8a0806c6a1bde5c8176670cd607163d
https://doi.org/10.1046/j.1432-1033.2003.03871.x
https://doi.org/10.1046/j.1432-1033.2003.03871.x
Autor:
Volker Kasche, Boris Galunsky
Publikováno v:
Advanced Synthesis & Catalysis. 344:1115-1119
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e3dd1c4707a7ec50123fe5d8d8f34db6
https://doi.org/10.1002/1615-4169(200212)344:10<1115::aid-adsc1115>3.0.co
https://doi.org/10.1002/1615-4169(200212)344:10<1115::aid-adsc1115>3.0.co
Autor:
Volker Kasche, Antje C. Spiess
Publikováno v:
Biotechnology Progress. 17:294-303
Confocal laser scanning microscopy was applied to measure the pH value in the carrier of immobilized enzymes during the enzyme-catalyzed synthesis. pH profiles with a high resolution are shown, with the pH increasing in the core of the particles. Sig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bd920496db205a880a2d52aa10227db4
https://doi.org/10.1021/bp000149e
https://doi.org/10.1021/bp000149e
Publikováno v:
Monatshefte fuer Chemie/Chemical Monthly. 131:623-632
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::657b1caa033e92dffa3a71055bf1ac02
https://doi.org/10.1007/s007060070090
https://doi.org/10.1007/s007060070090
Publikováno v:
Enzyme and Microbial Technology. 26:165-170
Intracellular proteolysis is an important mechanism for regulating the level of the periplasmic enzyme penicillin amidase in Escherichia coli. Evidence is presented that the active enzyme is localized in the periplasmic space and maturation of pro-en
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c32319f2460097291d3c0f718212faa1
https://doi.org/10.1016/s0141-0229(99)00130-1
https://doi.org/10.1016/s0141-0229(99)00130-1
Publikováno v:
Biotechnology Letters. 22:1815-1821
The activity of immobilised soybean lipoxygenase-1 (LOX-1) was studied in aqueous and supercritical carbon dioxide (SCCO2) media for the production of 13S-hydroperoxyoctadecadenoic acid (13S-HPODE). In SCCO2, it was optimal at 33 °C and 25 MPa. A hi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::76c8f0aa1ad2eddd7cc5d01df6fa1c83
https://doi.org/10.1023/a:1005626806321
https://doi.org/10.1023/a:1005626806321
Publikováno v:
Biotechnology Letters. 22:1727-1732
The yield of periplasmic enzyme, penicillin amidase (PA), from E. coli ATCC 11105 is regulated at the post-translational level by two competitive processes-intracellular proteolysis of newly synthesised pre-pro-PA (ppPA) in the cytoplasm and membrane
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4644bf35d34d9821c603745eaab06a85
https://doi.org/10.1023/a:1005600602162
https://doi.org/10.1023/a:1005600602162
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1433:327-334
The pH dependence of E (enantiomeric ratio or enantioselectivity, a quantitative measure for enzyme stereospecificity) was studied for penicillin amidase catalysed hydrolysis of charged enantiomeric substrates. Theoretical analysis shows that a pH de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ae9aa82f681abf4113ada3c67f9a014
https://doi.org/10.1016/s0167-4838(99)00130-2
https://doi.org/10.1016/s0167-4838(99)00130-2
Autor:
Volker Kasche, Wilhelm Tischer
Publikováno v:
Trends in Biotechnology. 17:326-335
The advantages of immobilized over soluble enzymes arise from their enhanced stability and ease of separation from the reaction media, leading to significant savings in enzyme consumption. Immobilization methods range from binding to prefabricated ca
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33448229d36da2e1d997eefdc780b34b
https://doi.org/10.1016/s0167-7799(99)01322-0
https://doi.org/10.1016/s0167-7799(99)01322-0