Utilizing epigenetic regulators to improve HSC-based lentiviral gene therapy.

Autor: Tajer P; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands., Karakaslar EO; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands.; Pattern Recognition & Bioinformatics, Delft University of Technology, Delft, The Netherlands., Canté-Barrett K; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands., Naber BAE; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands., Vloemans SA; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands., van Eggermond MCJA; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands., van der Hoorn ML; Department of Obstetrics, Leiden University Medical Center, Leiden, The Netherlands., van den Akker E; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands.; Pattern Recognition & Bioinformatics, Delft University of Technology, Delft, The Netherlands., Pike-Overzet K; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands., Staal FJT; Department of Immunology, Leiden University Medical Center, Leiden, The Netherlands.; Department of Pediatrics, Leiden University Medical Center, Leiden, The Netherlands.

Publikováno v: Blood advances [Blood Adv] 2024 Sep 24; Vol. 8 (18), pp. 4936-4947.

Single-cell immune profiling reveals thymus-seeding populations, T cell commitment, and multilineage development in the human thymus.

Autor: Cordes M; Department of Immunology, Leiden University Medical Center, Leiden, Netherlands.; Leiden Computational Biology Center, Leiden University Medical Center, Leiden, Netherlands., Canté-Barrett K; Department of Immunology, Leiden University Medical Center, Leiden, Netherlands.; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, Netherlands., van den Akker EB; Leiden Computational Biology Center, Leiden University Medical Center, Leiden, Netherlands.; Delft Bioinformatics Lab, Delft University of Technology, Delft, Netherlands.; Molecular Epidemiology, Leiden University Medical Center, Leiden, Netherlands., Moretti FA; Department of Immunology, Leiden University Medical Center, Leiden, Netherlands., Kiełbasa SM; Department of Biomedical Data Sciences, Leiden University Medical Center, Leiden, Netherlands., Vloemans SA; Department of Immunology, Leiden University Medical Center, Leiden, Netherlands., Garcia-Perez L; Department of Immunology, Leiden University Medical Center, Leiden, Netherlands., Teodosio C; Department of Immunology, Leiden University Medical Center, Leiden, Netherlands.; Centro de Investigación del Cáncer-Instituto de Biología Molecular y Celular del Cáncer (CIC-IBMCC, USAL-CSIC-FICUS), Department of Medicine, University of Salamanca, Salamanca, Spain., van Dongen JJM; Department of Immunology, Leiden University Medical Center, Leiden, Netherlands.; Centro de Investigación del Cáncer-Instituto de Biología Molecular y Celular del Cáncer (CIC-IBMCC, USAL-CSIC-FICUS), Department of Medicine, University of Salamanca, Salamanca, Spain., Pike-Overzet K; Department of Immunology, Leiden University Medical Center, Leiden, Netherlands., Reinders MJT; Leiden Computational Biology Center, Leiden University Medical Center, Leiden, Netherlands.; Delft Bioinformatics Lab, Delft University of Technology, Delft, Netherlands., Staal FJT; Department of Immunology, Leiden University Medical Center, Leiden, Netherlands.; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, Netherlands.; Department of Pediatrics, Leiden University Medical Center, Leiden, Netherlands.

Publikováno v: Science immunology [Sci Immunol] 2022 Nov 18; Vol. 7 (77), pp. eade0182. Date of Electronic Publication: 2022 Nov 11.

Axin2/Conductin Is Required for Normal Haematopoiesis and T Lymphopoiesis.

Autor: de Roo JJD; Department of Immunology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Chhatta A; Department of Immunology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Garcia-Perez L; Department of Immunology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Naber BAE; Department of Immunology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Vloemans SA; Department of Immunology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Salvatori DCF; Department of Clinical Sciences, Anatomy and Physiology Division, Faculty of Veterinary Medicine, Utrecht University, 3584 Utrecht, The Netherlands., Pike-Overzet K; Department of Immunology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Mikkers H; Department of Cell & Chemical Biology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands., Staal FJT; Department of Immunology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands.

Publikováno v: Cells [Cells] 2022 Aug 28; Vol. 11 (17). Date of Electronic Publication: 2022 Aug 28.

Successful Preclinical Development of Gene Therapy for Recombinase-Activating Gene-1-Deficient SCID.

Autor: Garcia-Perez L; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333ZA Leiden, the Netherlands., van Eggermond M; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333ZA Leiden, the Netherlands., van Roon L; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333ZA Leiden, the Netherlands., Vloemans SA; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333ZA Leiden, the Netherlands., Cordes M; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333ZA Leiden, the Netherlands., Schambach A; Institute of Experimental Hematology, Hannover Medical School, 30625 Hannover, Germany., Rothe M; Institute of Experimental Hematology, Hannover Medical School, 30625 Hannover, Germany., Berghuis D; Willem-Alexander Children's Hospital Department of Pediatrics, Leiden University Medical Center, 2333ZA Leiden, the Netherlands., Lagresle-Peyrou C; Biotherapy Clinical Investigation Center, Groupe Hospitalier Universitaire Ouest, Assistance Publique-Hôpitaux de Paris, INSERM CIC 1416, Paris, France.; Laboratory of Human Lymphohematopoiesis, INSERM UMR 1163, Imagine Institute and Paris Descartes University-Sorbonne Paris Cité, 75015 Paris, France.; Department of Biotherapy, Necker Children's Hospital, Assistance Publique-Hôpitaux de Paris, 75015 Paris, France., Cavazzana M; Biotherapy Clinical Investigation Center, Groupe Hospitalier Universitaire Ouest, Assistance Publique-Hôpitaux de Paris, INSERM CIC 1416, Paris, France.; Laboratory of Human Lymphohematopoiesis, INSERM UMR 1163, Imagine Institute and Paris Descartes University-Sorbonne Paris Cité, 75015 Paris, France.; Department of Biotherapy, Necker Children's Hospital, Assistance Publique-Hôpitaux de Paris, 75015 Paris, France., Zhang F; Molecular and Cellular Immunology, Great Ormond Street Institute of Child Health, and Great Ormond Street Hospital NHS Trust, London WC1N 1EH, UK., Thrasher AJ; Molecular and Cellular Immunology, Great Ormond Street Institute of Child Health, and Great Ormond Street Hospital NHS Trust, London WC1N 1EH, UK., Salvatori D; Central Laboratory Animal Facility, Pathology Unit, Leiden University Medical Center, 2333ZA Leiden, the Netherlands.; Department of Pharmacy, Leiden University Medical Center, 2333ZA Leiden, the Netherlands.; Pathogenesis and Treatment of Immune and Bone Diseases Unit, San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy.; Anatomy and Physiology Division, Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan1, 3584CL Utrecht, the Netherlands., Meij P; Department of Pharmacy, Leiden University Medical Center, 2333ZA Leiden, the Netherlands., Villa A; Pathogenesis and Treatment of Immune and Bone Diseases Unit, San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy., Van Dongen JJM; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333ZA Leiden, the Netherlands., Zwaginga JJ; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333ZA Leiden, the Netherlands., van der Burg M; Willem-Alexander Children's Hospital Department of Pediatrics, Leiden University Medical Center, 2333ZA Leiden, the Netherlands., Gaspar HB; Molecular and Cellular Immunology, Great Ormond Street Institute of Child Health, and Great Ormond Street Hospital NHS Trust, London WC1N 1EH, UK., Lankester A; Willem-Alexander Children's Hospital Department of Pediatrics, Leiden University Medical Center, 2333ZA Leiden, the Netherlands., Staal FJT; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333ZA Leiden, the Netherlands., Pike-Overzet K; Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333ZA Leiden, the Netherlands.

Publikováno v: Molecular therapy. Methods & clinical development [Mol Ther Methods Clin Dev] 2020 Mar 31; Vol. 17, pp. 666-682. Date of Electronic Publication: 2020 Mar 31 (Print Publication: 2020).

Development of an in vivo model to study clonal lineage relationships in hematopoietic cells using Brainbow2.1/Confetti mice.

Autor: de Roo JJ; Department of Immunohematology & Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Vloemans SA; Department of Immunohematology & Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Vrolijk H; Department of Cell & Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands., de Haas EF; Department of Immunohematology & Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands., Staal FJ; Department of Immunohematology & Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.

Publikováno v: Future science OA [Future Sci OA] 2019 Nov 18; Vol. 5 (10), pp. FSO427. Date of Electronic Publication: 2019 Nov 18.