Zobrazeno 1 - 10
of 215
pro vyhledávání: '"Vladislav V. Verkhusha"'
Autor:
Natalia V. Barykina, Erin M. Carey, Olena S. Oliinyk, Axel Nimmerjahn, Vladislav V. Verkhusha
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-16 (2024)
Abstract Near-infrared (NIR) probes are highly sought after as fluorescent tags for multicolor cellular and in vivo imaging. Here we develop small NIR fluorescent nanobodies, termed NIR-FbLAG16 and NIR-FbLAG30, enabling background-free visualization
Externí odkaz:
https://doaj.org/article/e98155c0e6a34182a01932e1e57b2510
Autor:
Kai Lu, Tetsuichi Wazawa, Tomoki Matsuda, Daria M. Shcherbakova, Vladislav V. Verkhusha, Takeharu Nagai
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-8 (2024)
Abstract Bacterial phytochromes are attractive molecular templates for engineering fluorescent proteins (FPs) because their near-infrared (NIR) emission significantly extends the spectral coverage of GFP-like FPs. Existing phytochrome-based FPs coval
Externí odkaz:
https://doaj.org/article/6e0be37afb3a45bc93e706a26513c174
Autor:
Francesca Pennacchietti, Jonatan Alvelid, Rodrigo A. Morales, Martina Damenti, Dirk Ollech, Olena S. Oliinyk, Daria M. Shcherbakova, Eduardo J. Villablanca, Vladislav V. Verkhusha, Ilaria Testa
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)
Abstract Photolabeling of intracellular molecules is an invaluable approach to studying various dynamic processes in living cells with high spatiotemporal precision. Among fluorescent proteins, photoconvertible mechanisms and their products are in th
Externí odkaz:
https://doaj.org/article/2d8a4d76185d4cd5bccf6a9421f0d8a7
Autor:
Ludmila A. Kasatkina, Chenshuo Ma, Mikhail E. Matlashov, Tri Vu, Mucong Li, Andrii A. Kaberniuk, Junjie Yao, Vladislav V. Verkhusha
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022)
Optogenetic tools can be used as in vivo imaging probes. Here the authors generate a loxP-BphP1 transgenic mouse to enable Cre-dependent temporal and spatial targeting of BphP1 expression in vivo; they show photoacoustic tomography of BphP1 expressio
Externí odkaz:
https://doaj.org/article/19a3d9b1e76a4652a521331cc3207f17
Autor:
Andrii A. Kaberniuk, Mikhail Baloban, Mikhail V. Monakhov, Daria M. Shcherbakova, Vladislav V. Verkhusha
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Current near-IR optogenetic systems to regulate transcription consist of a number of large protein components. Here the authors report a smaller single-component near-IR system, iLight, developed from a bacterial phytochrome that they use to control
Externí odkaz:
https://doaj.org/article/8acc6bf2a02e46e0b01259c899bbc2c0
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 10 (2022)
Nuclear transport in neurons differs from that in non-neuronal cells. Here we developed a non-opsin optogenetic tool (OT) for the nuclear export of a protein of interest induced by near-infrared (NIR) light. In darkness, nuclear import reverses the O
Externí odkaz:
https://doaj.org/article/c6a02215424e4cfaa49c03fb9d0d0360
Autor:
Mikhail E. Matlashov, Jorge Vera, Ludmila A. Kasatkina, Kamran Khodakhah, Vladislav V. Verkhusha
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 10 (2022)
Near-infrared (NIR) genetically encoded calcium indicators (GECIs) are becoming powerful tools for neuroscience. Because of their spectral characteristics, the use of NIR GECIs helps to avoid signal loss from the absorption by body pigments, light-sc
Externí odkaz:
https://doaj.org/article/02a6ad389ba54e1c93b687ac6ca7b67f
Autor:
Yusaku Hontani, Mikhail Baloban, Francisco Velazquez Escobar, Swetta A. Jansen, Daria M. Shcherbakova, Jörn Weißenborn, Miroslav Kloz, Maria Andrea Mroginski, Vladislav V. Verkhusha, John T. M. Kennis
Publikováno v:
Communications Chemistry, Vol 4, Iss 1, Pp 1-11 (2021)
Near-infrared fluorescent proteins engineered from bacterial phytochromes are important for deep-tissue imaging in vivo, but the mechanism through which they bind to chromophores is not fully understood. Here the authors structurally analyze biliverd
Externí odkaz:
https://doaj.org/article/27b7026a2cc7487aa17fc5c6ab44f7c5
Autor:
Mikhail E. Matlashov, Daria M. Shcherbakova, Jonatan Alvelid, Mikhail Baloban, Francesca Pennacchietti, Anton A. Shemetov, Ilaria Testa, Vladislav V. Verkhusha
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Monomeric near-infrared (NIR) fluorescent proteins (FPs) from bacterial phytochromes bring potential advantages, but their brightness in cells is lower than dimeric NIR FPs. Here the authors develop enhanced monomeric NIR FPs enabling imaging across
Externí odkaz:
https://doaj.org/article/06f6d420b5f14b9d96bca6c15df9a3a7
Autor:
Taras A. Redchuk, Maksim M. Karasev, Polina V. Verkhusha, Sara K. Donnelly, Maren Hülsemann, Jori Virtanen, Henna M. Moore, Maria K. Vartiainen, Louis Hodgson, Vladislav V. Verkhusha
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Optogenetic approaches to control protein-protein interactions usually require overexpression of the target proteins. Here the authors integrate intrabodies into near-infrared- and blue-light activatable optogenetic tools to control endogenous protei
Externí odkaz:
https://doaj.org/article/6f20a13284cc4e2a9ddb3638c37c57ca