Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Vladimir Y. Butnev"'
Autor:
Viktor Y. Butnev, Jeffrey V. May, Alan R. Brown, Tarak Sharma, Vladimir Y. Butnev, William K. White, David J. Harvey, George R. Bousfield
Publikováno v:
Frontiers in Endocrinology, Vol 13 (2022)
Follicle-stimulating hormone (FSH), an α/β heterodimeric glycoprotein hormone, consists of functionally significant variants resulting from the presence or absence of either one of two FSHβ subunit N-glycans. The two most abundant variants are ful
Externí odkaz:
https://doaj.org/article/65e5a5b4f54b4fa0b40688d3cc45d7e8
Autor:
Vladimir Y. Butnev, George R. Bousfield, David Harvey, Yasuaki Hiromasa, Jeffrey V. May, Viktor Y. Butnev
Publikováno v:
Molecular and Cellular Endocrinology. 382:989-997
Hypo-glycosylated hFSH(21/18) (possesses FSHβ(21) and FSHβ(18)bands) was isolated from hLH preparations by immunoaffinity chromatography followed by gel filtration. Fully-glycosylated hFSH(24) was prepared by combining the fully-glycosylated FSHβ(
Publikováno v:
Molecular and cellular endocrinology. 426
Follicle-stimulating hormone (FSH) is a gonadotrope-derived heterodimeric glycoprotein. Both the common α- and hormone-specific β subunits contain Asn-linked N-glycan chains. Recently, macroheterogeneous FSH glycoforms consisting of β-subunits tha
Autor:
Jennifer Huneidi, Naomi E. Rance, Wendy J. Walton, Van T. Nguyen, David Harvey, Vladimir Y. Butnev, V. S. Kumar Kolli, Vinod Singh, George R. Bousfield
Publikováno v:
Molecular and Cellular Endocrinology. :40-48
Human FSH exists as two major glycoforms designated, tetra-glycosylated and di-glycosylated hFSH. The former possesses both alpha- and beta-subunit carbohydrates while the latter possesses only alpha-subunit carbohydrate. Western blotting differentia
Publikováno v:
The FASEB Journal. 29
Autor:
Aaron Smalter Hall, Monica A. Rueda-Santos, David Harvey, George R. Bousfield, Vladimir Y. Butnev, Alan Brown
Publikováno v:
Journal of glycomicslipidomics. 4
FSH glycosylation macroheterogeneity in pituitary and urinary hFSH samples was evaluated by Western blotting. Microheterogeneity in two highly purified urinary and pituitary hFSH preparations was evaluated by nano-electrospray mass spectrometry of pe
Production, purification, and characterization of recombinant hFSH glycoforms for functional studies
Autor:
William K. White, Jeffrey V. May, Patrick Tran, Vladimir Y. Butnev, David Harvey, George R. Bousfield, Viktor Y. Butnev, Bin Shuai, Alan Brown, Aaron Smalter Hall
Previously, our laboratory demonstrated the existence of a β-subunit glycosylation-deficient human FSH glycoform, hFSH(21). A third variant, hFSH(18), has recently been detected in FSH glycoforms isolated from purified pituitary hLH preparations. Hu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2180087788bcde654c52241d39b7552a
https://europepmc.org/articles/PMC4378652/
https://europepmc.org/articles/PMC4378652/
Publikováno v:
Journal of Glycomics & Lipidomics.
Glycans from six highly purified hFSH preparations were released by peptide-N-glycanase digestion and analyzed by negative mode nano-ESI mass spectrometry before and after neuraminidase digestion. Pituitary glycan structures were mainly high-mannose,
Autor:
Vinod Singh, George R. Bousfield, Vladimir Y. Butnev, Ciann M. Gray, Suzanne Westfall, John S. Davis, Van T. Nguyen, James A. Dias
Publikováno v:
Molecular and Cellular Endocrinology. 199:73-86
Lentil lectin-bound, fucose-enriched hTSH was reported to stimulate both cAMP and inositol phosphate (IP) intracellular signalling pathways, whereas fucose-depleted hTSH stimulated only the cAMP pathway. Gonadotropins activate the cAMP pathway and in
Autor:
Vinod Singh, William T. Moore, Wendy J. Walton, Vladimir Y. Butnev, Van T. Nguyen, George R. Bousfield
Publikováno v:
The Journal of Clinical Endocrinology & Metabolism. 86:3675-3685
Human FSH consists of a mixture of isoforms that can be separated on the basis of differences in negative charge conferred by variations in the numbers of sialic acid residues that terminate oligosaccharide branches. Western analysis of human FSH iso