Výsledky vyhledávání - "Vladimir Riazanski"

Akademický článek

Chloride/proton antiporters ClC3 and ClC5 support bone formation in mice

Autor: Irina L. Tourkova, Quitterie C. Larrouture, Silvia Liu, Jianhua Luo, Katherine E. Shipman, Kelechi M. Onwuka, Ora A. Weisz, Vladimir Riazanski, Deborah J. Nelson, Matthew L. MacDonald, Paul H. Schlesinger, Harry C. Blair

Publikováno v: Bone Reports, Vol 21, Iss , Pp 101763- (2024)

Acid transport is required for bone synthesis by osteoblasts. The osteoblast basolateral surface extrudes acid by Na+/H+ exchange, but apical proton uptake is undefined. We found high expression of the Cl−/H+ exchanger ClC3 at the bone apical surfa

Externí odkaz: https://doaj.org/article/bf7bf14d1982441993e7c2bea477f974

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Akademický článek

Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform

Autor: Vladimir Riazanski, Gerardo Mauleon, Kilean Lucas, Samuel Walker, Adriana M. Zimnicka, James L. McGrath, Deborah J. Nelson

Publikováno v: Communications Biology, Vol 5, Iss 1, Pp 1-13 (2022)

Riazanski et al describe a platform to capture extracellular vesicles (EVs) using a nanoporous silicon nitride membrane, investigate the expression of NHE1 protein on the surface of EVs and monitor the transport of Na+ and H+ at the single EV level.

Externí odkaz: https://doaj.org/article/98864c1740fb4e14ba8f46b059eb5d8e

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Akademický článek

Phagosomal chloride dynamics in the alveolar macrophage

Autor: Vladimir Riazanski, Gerardo Mauleon, Adriana M. Zimnicka, Si Chen, Deborah J. Nelson

Publikováno v: iScience, Vol 25, Iss 1, Pp 103636- (2022)

Summary: Acidification in intracellular organelles is tightly linked to the influx of Cl− counteracting proton translocation by the electrogenic V-ATPase. We quantified the dynamics of Cl− transfer accompanying cargo incorporation into single pha

Externí odkaz: https://doaj.org/article/5852ba0c304f43609f8a5f9e6960e248

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Akademický článek

Kinetic Separation of Oxidative and Non-oxidative Metabolism in Single Phagosomes from Alveolar Macrophages: Impact on Bacterial Killing

Autor: Vladimir Riazanski, Zihao Sui, Deborah J. Nelson

Publikováno v: iScience, Vol 23, Iss 11, Pp 101759- (2020)

Summary: The relative contribution of the two phagosomal catabolic processes, oxidative and metabolic, was assessed in the killing of Pseudomonas aeruginosa in phagosomes of alveolar macrophages (AMs) from wild-type (p47-phox+/+) or NOX-defective (p4

Externí odkaz: https://doaj.org/article/c8da26372ff2425f91c772e7fdea8f53

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Growth and mineralization of osteoblasts from mesenchymal stem cells on microporous membranes: Epithelial-like growth with transmembrane resistance and pH gradient

Autor: Irina L. Tourkova, Vladimir Riazanski, Kelechi M. Onwuka, Jonathan Franks, Steven F. Dobrowolski, Harry C. Blair, Quitterie C. Larrouture, Donna B. Stolz, Deborah J. Nelson, Paul H. Schlesinger

Publikováno v: Biochem Biophys Res Commun

Osteoblasts in vivo form an epithelial-like layer with tight junctions between cells. Bone formation involves mineral transport into the matrix and acid transport to balance pH levels. To study the importance of the pH gradient in vitro, we used Tran

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6501762965767ba5379d3d4a27f7cc5
https://doi.org/10.1016/j.bbrc.2021.09.075

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Cellular and extracellular matrix of bone, with principles of synthesis and dependency of mineral deposition on cell membrane transport

Autor: Harry C. Blair, Paul H. Schlesinger, Irina L. Tourkova, Donna B. Stolz, Evan C. Ray, Vladimir Riazanski, Deborah J. Nelson

Publikováno v: Am J Physiol Cell Physiol

Bone differs from other connective tissues; it is isolated by a layer of osteoblasts that are connected by tight and gap junctions. This allows bone to create dense lamellar type I collagen, control pH, mineral deposition, and regulate water content

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a32ca7225f648559098218f08a2afd17
https://doi.org/10.1152/ajpcell.00120.2019

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Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform

Autor: Vladimir Riazanski, Gerardo Mauleon, Kilean Lucas, Samuel Walker, Adriana M. Zimnicka, James L. McGrath, Deborah J. Nelson

Publikováno v: Communications Biology
Communications Biology, Vol 5, Iss 1, Pp 1-13 (2022)

Extracellular vesicles (EVs) are cell-derived membranous structures carrying transmembrane proteins and luminal cargo. Their complex cargo requires pH stability in EVs while traversing diverse body fluids. We used a filtration-based platform to captu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef12546a8ce6e2b9ee156b95a0ed1f56
https://pubmed.ncbi.nlm.nih.gov/35013561

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Kinetic Separation of Oxidative and Non-oxidative Metabolism in Single Phagosomes from Alveolar Macrophages: Impact on Bacterial Killing

Autor: Deborah J. Nelson, Vladimir Riazanski, Zihao Sui

Publikováno v: iScience
iScience, Vol 23, Iss 11, Pp 101759-(2020)

Summary The relative contribution of the two phagosomal catabolic processes, oxidative and metabolic, was assessed in the killing of Pseudomonas aeruginosa in phagosomes of alveolar macrophages (AMs) from wild-type (p47-phox+/+) or NOX-defective (p47

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::557b17b235904c27828e93c60f586e18
http://europepmc.org/articles/PMC7677711

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