Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Vladimir N. Ksenzenko"'
Autor:
Michael G. Shlyapnikov, A. I. Krutilina, Anatoly S. Glukhov, A. V. Kaliman, Vladimir N. Ksenzenko
Publikováno v:
Молекулярная биология. :3-9
A new series of heat-stable (st) mutants of bacteriophage T5, which contains deletions in the tRNA gene region, has been isolated. An accurate mapping of the deletion boundaries for more than 30 mutants of phage T5 has been carried out. As a result o
Autor:
Vladimir N. Ksenzenko, Tatiana V. Ivashina, Maxim A. Arzhanov, Leonid M. Vinokurov, Lyubov Shaloiko, Natalia V. Rudenko, Lilia L. Sinegina, Oleg S. Morenkov
Publikováno v:
Journal of Biochemical and Biophysical Methods. 70:605-611
The effective new variant of "sandwich" bioluminescent enzyme immunoassay (BEIA) for the sensitive detection of glycoprotein B (gB) of pseudorabies virus (PrV) was presently developed. The high affinity interaction of barnase-barstar protein pair and
Autor:
Tanya V. Ivashina, Gennady V. Semisotnov, Andrey Yu. Gorokhovatsky, Nils Burkhardt, Yuli B. Alakhov, Leonid M. Vinokurov, Victor V. Marchenkov, Vladimir N. Ksenzenko, Natalia V. Rudenko
Publikováno v:
Biochemical and Biophysical Research Communications. 320:703-711
The bioluminescence emitted by Aequorea victoria jellyfish is greenish while its single bioluminescent photoprotein aequorin emits blue light. This phenomenon may be explained by a bioluminescence resonance energy transfer (BRET) from aequorin chromo
Publikováno v:
Molecular Biology. 38:530-537
Site-specific endonucleases F-TflI, F-TflII, and F-TflIV have been revealed, which belong to the H-N-H family and are encoded by ORFs located in the tRNA gene region of bacteriophage T5. It has been shown that endonuclease F-TflIV introduces a double
Autor:
Vladimir N. Ksenzenko, Olesya Vakhrusheva, M. G. Pyatibratov, Alexander Serganov, I. N. Meshcheryakova, Inna Serganova, Antonina L. Metlina, O. V. Fedorov
Publikováno v:
Journal of Bacteriology. 184:318-322
We have determined the nucleotide sequence of a flagellin gene locus from the haloalkaliphilic archaeon Natrialba magadii , identified the gene products among proteins forming flagella, and demonstrated cotranscription of the genes. Based on the sequ
Autor:
Vitaly S. Skosyrev, Yu.B. Alakhov, Vladimir N. Ksenzenko, Tanya V. Ivashina, Natalia V. Rudenko, A. Yu. Gorokhovatsky, Leonid M. Vinokurov
Publikováno v:
Russian Journal of Bioorganic Chemistry. 27:323-329
Recombinant plasmids containing genes for the green fluorescent protein (GFP) from Aequorea victoriaand the photoprotein obelin from Obelia longissimalinked in-frame by inserts differing in nucleotides sequences were constructed. The expression of th
Autor:
Michael A. Djordjevic, Nelson Guerreiro, Tanya V. Ivashina, Vladimir N. Ksenzenko, Barry G. Rolfe
Publikováno v:
Journal of Bacteriology. 182:4521-4532
The protein expression profiles of Rhizobium leguminosarum strains in response to specific genetic perturbations in exopolysaccharide (EPS) biosynthesis genes were examined using two-dimensional gel electrophoresis. Lesions in either pssA , pssD , or
Autor:
Robert F. Bonsall, Alexander M. Boronin, R. James Cook, Dmitri V. Mavrodi, Vladimir N. Ksenzenko, Linda S. Thomashow
Publikováno v:
Europe PubMed Central
Pseudomonas fluorescens 2-79 produces the broad-spectrum antibiotic phenazine-1-carboxylic acid (PCA), which is active against a variety of fungal root pathogens. In this study, seven genes designated phzABCDEFG that are sufficient for synthesis of P
Publikováno v:
FEBS Letters. 426:135-139
Kinetic parameters of aminoacylation by E. coli phenylalanyl-tRNA synthetase vary for phage T5 tRNA(Phe) gene transcript from 0.950 to 2.545 microM for Km and from 550 to 400 min(-1) for kcat. To reveal the source of this variability for various RNA
Autor:
Andrey V. Kajava, Vladimir N. Ksenzenko, Z. N. Karamysheva, A. L. Karamyshev, M. A. Nesmeyanova
Publikováno v:
Journal of Molecular Biology. 277:859-870
A wide range (69) of mutant Escherichia coli alkaline phosphatases with single amino acid substitutions at positions from -5 to +1 of the signal peptide were obtained for studying protein processing as a function of the primary structure of the cleav