Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Vladimir N. Barai"'
Publikováno v:
Biotechnology Letters. 26:1847-1850
An improved method to transform nucleosides into 5'-monophosphates using nucleoside phosphotransferase from Erwinia herbicola is reported. The method is based on the shift in the equilibrium state of the reaction to the formation of desired product d
Autor:
A. I. Zinchenko, Olga I. Titovich, Elena N. Kalinichenko, Elena B. Rubinova, Sergei V. Kvach, Vladimir N. Barai, Igor A. Mikhailopulo
Publikováno v:
Helvetica Chimica Acta. 86:2827-2832
Treatment of adenosine with PSCl3 in trimethyl phosphate gave, after ion-exchange chromatography, adenosine-5′-O-monophosphate (AMP; 28%) and adenosine-5′-O-monothiophosphate (AMPS; 48%). AMPS was studied as a thiophosphate residue donor in an en
Publikováno v:
Biotechnology Techniques. 10:875-878
Ribonucleosides can be produced by calcium-catalyzed cleavage of RNA followed by dephosphorylation of calcium salts of mononucleotides by intact mycelium of Spicaria violacea as a biocatalyst. Under optimal conditions the efficiency of degradation of
Autor:
Vladimir N. Barai, A. I. Zinchenko, L. M. Zalashko, Igor A. Mikhailopulo, Ludmilla A. Eroshevskaya
Publikováno v:
Biotechnology Letters. 17:599-602
The title compound was prepared by a three-stage enzymatic procedure consisting of (i) RNA hydrolysis to a mixture of ribonucleosides using intact mycelium of Spicaria violacea, (ii) transribosylation of exogenous adenine employing whole cells of Esc
Autor:
Vladimir N. Barai, Zygmunt Kazimierczuk, Igor A. Mikhailopulo, Ludmilla A. Eroshevskaya, A. I. Zinchenko, Valeria V. Romanova
Publikováno v:
Nucleosides, Nucleotides and Nucleic Acids. 14:477-480
Substrate activity of a broad spectrum of derivatives of benzimidazole in the reaction of enzymatic ribo- and 2-deoxyribosylation catalyzed by purine nucleoside phosphorylase of whole cells of E. coli BMT-1D/1A has been studied. Guanosine or 2′-deo
Autor:
Erik De Clercq, Igor A. Mikhailopulo, A. I. Zinchenko, Vladimir N. Barai, Ludmilla A. Eroshevskaya, Elena V. Zhernosek, Jan Balzarini
Publikováno v:
Nucleosides, Nucleotides and Nucleic Acids. 22:751-753
9-(3-Deoxy-beta-D-erythro-pentofuranosyl)-2,6-diaminopurine (2) was synthesized by an enzymatic transglycosylation of 2,6-diaminopurine using 3'-deoxycytidine (1) as a donor of the sugar moiety. Nucleoside 2 was transformed to 3'-deoxy guanosine (3),
Autor:
Vladimir N. Barai, A. I. Zinchenko, E. V. Vaaks, E. I. Kvasyuk, S. B. Bokut, G. V. Zaitseva, Igor A. Mikhailopulo
Publikováno v:
Nucleosides and Nucleotides. 13:819-834
Treatment of O2, 3′-anhydro-5′-O-trityl derivatives of thymidine (1) and 2′-deoxyuridine (2) with lithium azide in dimethylformamide at 150 °C resulted in the formation of the corresponding isomeric 3′-azido-2′, 3′-dideoxy-5′-O-trityl-
Autor:
A. I. Zinchenko, Elena N. Kalinichenko, Igor A. Mikhailopulo, S. B. Bokut, Vladimir N. Barai, Zygmunt Kazimierczuk
Publikováno v:
Nucleosides and Nucleotides. 12:417-422
The title compound have been synthesized by an enzymatic trans-2′-deoxyribosylation of 2-chloroadenine using the whole cells of E. coli BMT-1D/1A as a biocatalyst and 2′-deoxyguanosine as a donor of glycosyl moiety.
Autor:
S. B. Bokut, A. I. Zinchenko, E. I. Kvasyuk, Igor A. Mikhailopulo, N. V. Dudchik, Vladimir N. Barai
Publikováno v:
Biotechnology Letters. 13:87-90
The title compound was prepared by a two step enzymatic procedure consisting of DNA hydrolysis to the mixture of 2′-deoxynucleosides followed by a transdeoxyribosilation of exogenous adenine.
Autor:
Vladimir N. Barai, Yu. V. Belyaeva, S. B. Bokut, Igor A. Mikhailopulo, N. V. Dudochik, A. J. Zinchenko
Publikováno v:
Biotechnology Letters. 12:341-346
2′-Deoxythymidine was synthesized by an enzymatic transdeoxyribosylation of thymine using either (i) dGuo, dCyd or dAdo, or (ii) the mixture of the same 2′-deoxynucleosides resulting from enzymatic hydrolysis of DNA as donors of 2-deoxyribofurano