Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Vladimir Mekler"'
Publikováno v:
Viruses, Vol 14, Iss 3, p 555 (2022)
Salmonella enterica serovar Newport bacteriophage 7-11 shares 41 homologous ORFs with Escherichia coli phage phiEco32, and both phages encode a protein similar to bacterial RNA polymerase promoter specificity σ subunit. Here, we investigated the tem
Externí odkaz:
https://doaj.org/article/5cb36e99b795471fad83a779f7d8eb35
Autor:
Evgeny Klimuk, Vladimir Mekler, Darya Lavysh, Marina Serebryakova, Natalia Akulenko, Konstantin Severinov
Publikováno v:
Viruses, Vol 12, Iss 8, p 807 (2020)
The Escherichia coli bacteriophage T5 has three temporal classes of genes (pre-early, early, and late). All three classes are transcribed by host RNA polymerase (RNAP) containing the σ70 promoter specificity subunit. Molecular mechanisms responsible
Externí odkaz:
https://doaj.org/article/900e1fd1db384068b2ccf695f35ded07
Autor:
Yu Zhang, David Degen, Mary X Ho, Elena Sineva, Katherine Y Ebright, Yon W Ebright, Vladimir Mekler, Hanif Vahedian-Movahed, Yu Feng, Ruiheng Yin, Steve Tuske, Herbert Irschik, Rolf Jansen, Sonia Maffioli, Stefano Donadio, Eddy Arnold, Richard H Ebright
Publikováno v:
eLife, Vol 3 (2014)
Using a combination of genetic, biochemical, and structural approaches, we show that the cyclic-peptide antibiotic GE23077 (GE) binds directly to the bacterial RNA polymerase (RNAP) active-center ‘i’ and ‘i+1’ nucleotide binding sites, preven
Externí odkaz:
https://doaj.org/article/f4f3d502427d47b09fde0b71d754abf6
Autor:
Regina Shebanova, Natalia Nikitchina, Nikita Shebanov, Vladimir Mekler, Konstantin Kuznedelov, Egor Ulashchik, Ruslan Vasilev, Olga Sharko, Vadim Shmanai, Ivan Tarassov, Konstantin Severinov, Nina Entelis, Ilya Mazunin
Publikováno v:
Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 2021, ⟨10.1093/nar/gkab1227⟩
Nucleic Acids Research, Oxford University Press, 2021, ⟨10.1093/nar/gkab1227⟩
CRISPR RNAs (crRNAs) that direct target DNA cleavage by Type V Cas12a nucleases consist of constant repeat-derived 5′-scaffold moiety and variable 3′-spacer moieties. Here, we demonstrate that removal of most of the 20-nucleotide scaffold has onl
Autor:
Nina Entelis, Konstantin Severinov, Vadim V. Shmanai, Natalia Nikitchina, Ilya O. Mazunin, Nikita Shebanov, Regina Tkach, Vladimir Mekler, Egor A. Ulashchik, Ivan Tarassov, O. L. Sharko
CRISPR RNAs (crRNAs) directing target DNA cleavage by type V-A Cas12a nucleases consist of repeat-derived 5’-scaffold moiety and 3’-spacer moiety. We demonstrate that removal of most of the 20-nucleotide scaffold has only a slight effect on in vi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dc73c67a15281bf4fe8b2dc61d75caf
https://doi.org/10.1101/2020.12.21.423781
https://doi.org/10.1101/2020.12.21.423781
Autor:
Vladimir Mekler, Evgeny Klimuk, Marina V. Serebryakova, Natalia Akulenko, Darya Lavysh, Konstantin Severinov
Publikováno v:
Viruses, Vol 12, Iss 807, p 807 (2020)
Viruses
Volume 12
Issue 8
Viruses
Volume 12
Issue 8
The Escherichia coli bacteriophage T5 has three temporal classes of genes (pre-early, early, and late). All three classes are transcribed by host RNA polymerase (RNAP) containing the &sigma
70 promoter specificity subunit. Molecular mechanisms r
70 promoter specificity subunit. Molecular mechanisms r
Autor:
Evgeny, Klimuk, Vladimir, Mekler, Darya, Lavysh, Marina, Serebryakova, Natalia, Akulenko, Konstantin, Severinov
Publikováno v:
Viruses
The Escherichia coli bacteriophage T5 has three temporal classes of genes (pre-early, early, and late). All three classes are transcribed by host RNA polymerase (RNAP) containing the σ70 promoter specificity subunit. Molecular mechanisms responsible
Autor:
Leonid Minakhin, Ishita Jain, Vladimir Mekler, Ekaterina Semenova, Natalia Rubanova, Konstantin Severinov, Vasily Sitnik
Publikováno v:
RNA Biology
Target binding by CRISPR-Cas ribonucleoprotein effectors is initiated by the recognition of double-stranded PAM motifs by the Cas protein moiety followed by destabilization, localized melting, and interrogation of the target by the guide part of CRIS
Autor:
Konstantin Severinov, Daria Esyunina, Vladimir Mekler, Andrey Kulbachinskiy, Ivan Petushkov, Danil Pupov
Publikováno v:
Biochemical Journal. 474:4053-4064
In bacterial RNA polymerase (RNAP), conserved region 3.2 of the σ subunit was proposed to contribute to promoter escape by interacting with the 5′-end of nascent RNA, thus facilitating σ dissociation. RNAP activity during transcription initiation
Autor:
Shigeyuki Yokoyama, Wei Yang Ooi, Yuko Murayama, Shun-ichi Sekine, Konstantin Severinov, Vladimir Mekler, Leonid Minakhin
Publikováno v:
Nucleic Acids Research
RNA polymerase (RNAP) is a major target of gene regulation. Thermus thermophilus bacteriophage P23–45 encodes two RNAP binding proteins, gp39 and gp76, which shut off host gene transcription while allowing orderly transcription of phage genes. We p