Efficient CRISPR/Cas9-mediated gene disruption in the tetraploid protist Giardia intestinalis

Autor: Vendula Horáčková, Luboš Voleman, Kari D. Hagen, Markéta Petrů, Martina Vinopalová, Filip Weisz, Natalia Janowicz, Lenka Marková, Alžběta Motyčková, Vladimíra Najdrová, Pavla Tůmová, Scott C. Dawson, Pavel Doležal

Publikováno v: Open Biology, Vol 12, Iss 4 (2022)

CRISPR/Cas9-mediated genome editing has become an extremely powerful technique used to modify gene expression in many organisms, including parasitic protists. Giardia intestinalis, a protist parasite that infects approximately 280 million people arou

Externí odkaz: https://doaj.org/article/d96837c111014c348aaa6b61ad7d506b

Giardia intestinalis mitosomes undergo synchronized fission but not fusion and are constitutively associated with the endoplasmic reticulum

Autor: Luboš Voleman, Vladimíra Najdrová, Ásgeir Ástvaldsson, Pavla Tůmová, Elin Einarsson, Zdeněk Švindrych, Guy M. Hagen, Jan Tachezy, Staffan G. Svärd, Pavel Doležal

Publikováno v: BMC Biology, Vol 15, Iss 1, Pp 1-16 (2017)

Abstract Background Mitochondria of opisthokonts undergo permanent fission and fusion throughout the cell cycle. Here, we investigated the dynamics of the mitosomes, the simplest forms of mitochondria, in the anaerobic protist parasite Giardia intest

Externí odkaz: https://doaj.org/article/e2b6b9225b27496b8d9cb560e606c488

The late ISC pathway interactome reveals mitosomal-cytoplasmic crosstalk in Giardia intestinalis

Autor: Alžběta Motyčková, Luboš Voleman, Vladimíra Najdrová, Lenka Marková, Martin Benda, Vít Dohnálek, Natalia Janowicz, Ronald Malych, Róbert Šuťák, Thijs J. G. Ettema, Staffan Svärd, Courtney W. Stairs, Pavel Doležal

Mitochondrial metabolism is entirely dependent on the biosynthesis of the [4Fe-4S] clusters, which are part of the subunits of the respiratory chain. The mitochondrial late ISC pathway mediates the formation of these clusters from simpler [2Fe-2S] mo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::68677ab564ee0137d3d55ad210e70bf0
https://doi.org/10.1101/2022.08.01.502261

Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3

Autor: Michelle Y, Fry, Vladimíra, Najdrová, Ailiena O, Maggiolo, Shyam M, Saladi, Pavel, Doležal, William M, Clemons

Publikováno v: Nature structuralmolecular biology. 29(8)

Tail-anchored (TA) membrane proteins, accounting for roughly 2% of proteomes, are primarily targeted posttranslationally to the endoplasmic reticulum membrane by the guided entry of TA proteins (GET) pathway. For this complicated process, it remains

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::52f8d58e5a23372136e10d95e08ed560
https://pubmed.ncbi.nlm.nih.gov/35851188

Live imaging of mitosomes and hydrogenosomes by HaloTag technology

Autor: Jan Tachezy, Maximiliano G. De Napoli, Luboš Voleman, Daria Van Tyne, Christine S. Hopp, Michaela Marcinčiková, Vladimíra Najdrová, Eva Martincová, Pavel Doležal, David E. Sanin, Dawn M. Walker, Shiri Eshar, Melisa Gualdron, Dumizulu L. Tembo

Publikováno v: PLoS ONE, Vol 7, Iss 4, p e36314 (2012)
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
PLoS ONE
PLoS One, Vol. 7, no. 4 (2012)

Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fecb00c39c3a37621d576079ff181b09
http://europepmc.org/articles/PMC3338651?pdf=render