iCLOTS: open-source, artificial intelligence-enabled software for analyses of blood cells in microfluidic and microscopy-based assays

Autor: Meredith E. Fay, Oluwamayokun Oshinowo, Elizabeth Iffrig, Kirby S. Fibben, Christina Caruso, Scott Hansen, Jamie O. Musick, José M. Valdez, Sally S. Azer, Robert G. Mannino, Hyoann Choi, Dan Y. Zhang, Evelyn K. Williams, Erica N. Evans, Celeste K. Kanne, Melissa L. Kemp, Vivien A. Sheehan, Marcus A. Carden, Carolyn M. Bennett, David K. Wood, Wilbur A. Lam

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-16 (2023)

Abstract While microscopy-based cellular assays, including microfluidics, have significantly advanced over the last several decades, there has not been concurrent development of widely-accessible techniques to analyze time-dependent microscopy data i

Externí odkaz: https://doaj.org/article/3240155307a64ee7b82312b788ad694a

Loss-of-function genomic variants highlight potential therapeutic targets for cardiovascular disease

Autor: Jonas B. Nielsen, Oren Rom, Ida Surakka, Sarah E. Graham, Wei Zhou, Tanmoy Roychowdhury, Lars G. Fritsche, Sarah A. Gagliano Taliun, Carlo Sidore, Yuhao Liu, Maiken E. Gabrielsen, Anne Heidi Skogholt, Brooke Wolford, William Overton, Ying Zhao, Jin Chen, He Zhang, Whitney E. Hornsby, Akua Acheampong, Austen Grooms, Amanda Schaefer, Gregory J. M. Zajac, Luis Villacorta, Jifeng Zhang, Ben Brumpton, Mari Løset, Vivek Rai, Pia R. Lundegaard, Morten S. Olesen, Kent D. Taylor, Nicholette D. Palmer, Yii-Der Chen, Seung H. Choi, Steven A. Lubitz, Patrick T. Ellinor, Kathleen C. Barnes, Michelle Daya, Nicholas Rafaels, Scott T. Weiss, Jessica Lasky-Su, Russell P. Tracy, Ramachandran S. Vasan, L. Adrienne Cupples, Rasika A. Mathias, Lisa R. Yanek, Lewis C. Becker, Patricia A. Peyser, Lawrence F. Bielak, Jennifer A. Smith, Stella Aslibekyan, Bertha A. Hidalgo, Donna K. Arnett, Marguerite R. Irvin, James G. Wilson, Solomon K. Musani, Adolfo Correa, Stephen S. Rich, Xiuqing Guo, Jerome I. Rotter, Barbara A. Konkle, Jill M. Johnsen, Allison E. Ashley-Koch, Marilyn J. Telen, Vivien A. Sheehan, John Blangero, Joanne E. Curran, Juan M. Peralta, Courtney Montgomery, Wayne H-H Sheu, Ren-Hua Chung, Karen Schwander, Seyed M. Nouraie, Victor R. Gordeuk, Yingze Zhang, Charles Kooperberg, Alexander P. Reiner, Rebecca D. Jackson, Eugene R. Bleecker, Deborah A. Meyers, Xingnan Li, Sayantan Das, Ketian Yu, Jonathon LeFaive, Albert Smith, Tom Blackwell, Daniel Taliun, Sebastian Zollner, Lukas Forer, Sebastian Schoenherr, Christian Fuchsberger, Anita Pandit, Matthew Zawistowski, Sachin Kheterpal, Chad M. Brummett, Pradeep Natarajan, David Schlessinger, Seunggeun Lee, Hyun Min Kang, Francesco Cucca, Oddgeir L. Holmen, Bjørn O. Åsvold, Michael Boehnke, Sekar Kathiresan, Goncalo R. Abecasis, Y. Eugene Chen, Cristen J. Willer, Kristian Hveem

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)

Drugs targeting cardiovascular disease (CVD) can have negative consequences for liver function. Here, the authors combine genome wide analyses on 69,479 individuals to identify loss-of-function variants with beneficial effects on CVD-related traits w

Externí odkaz: https://doaj.org/article/2eecf9230b454b46b95b45b0f20ddd2e

End points for sickle cell disease clinical trials: patient-reported outcomes, pain, and the brain

Autor: Ann T. Farrell, Julie Panepinto, C. Patrick Carroll, Deepika S. Darbari, Ankit A. Desai, Allison A. King, Robert J. Adams, Tabitha D. Barber, Amanda M. Brandow, Michael R. DeBaun, Manus J. Donahue, Kalpna Gupta, Jane S. Hankins, Michelle Kameka, Fenella J. Kirkham, Harvey Luksenburg, Shirley Miller, Patricia Ann Oneal, David C. Rees, Rosanna Setse, Vivien A. Sheehan, John Strouse, Cheryl L. Stucky, Ellen M. Werner, John C. Wood, William T. Zempsky

Publikováno v: Blood Advances, Vol 3, Iss 23, Pp 3982-4001 (2019)

Abstract: To address the global burden of sickle cell disease (SCD) and the need for novel therapies, the American Society of Hematology partnered with the US Food and Drug Administration to engage the work of 7 panels of clinicians, investigators, a

Externí odkaz: https://doaj.org/article/94dcc43affc1405aa07a25c3738bac96

Concurrent Assessment of Deformability and Adhesiveness of Sickle Red Blood Cells by Measuring Perfusion of an Adhesive Artificial Microvascular Network

Autor: Madeleine Lu, Celeste K. Kanne, Riley C. Reddington, Dalia L. Lezzar, Vivien A. Sheehan, Sergey S. Shevkoplyas

Publikováno v: Frontiers in Physiology, Vol 12 (2021)

Biomarker development is a key clinical research need in sickle cell disease (SCD). Hemorheological parameters are excellent candidates as abnormal red blood cell (RBC) rheology plays a critical role in SCD pathophysiology. Here we describe a microfl

Externí odkaz: https://doaj.org/article/b24aa7c0e348458b83b42863c18d319d

Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editing

Autor: So Hyun Park, Mingming Cao, Yidan Pan, Timothy H. Davis, Lavanya Saxena, Harshavardhan Deshmukh, Yilei Fu, Todd Treangen, Vivien A. Sheehan, Gang Bao

Publikováno v: Science advances. 8(42)

Most genome editing analyses to date are based on quantifying small insertions and deletions. Here, we show that CRISPR-Cas9 genome editing can induce large gene modifications, such as deletions, insertions, and complex local rearrangements in differ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::185888a3783d87264d35c1958c53b894
https://pubmed.ncbi.nlm.nih.gov/36269834