Výsledky vyhledávání - "Vivian Granger"

Evaluation of leukocyte stabilisation in TransFix®-treated blood samples by flow cytometry and transmission electron microscopy

Autor: Vivian Granger, Sabrina Burattini, Loris Zamai, Barbara Canonico, Elisabetta Falcieri, Ferdinando Mannello, Stefano Papa, John T. Reilly, C. Felici, David Barnett, Pietro Gobbi

Publikováno v: Journal of immunological methods
295 (2004): 67–78. doi:10.1016/j.jim.2004.09.013
info:cnr-pdr/source/autori:B. CANONICO, L. ZAMAI, S. BURATTINI, V. GRANGER, F. MANNELLO, P. GOBBI, C. FELICI, E. FALCIERI, J.T. REILLY, D. BARNETT, S. PAPA,/titolo:Evaluation of leukocyte stabilisation in TransFix(R)-treated blood samples by flow cytometry and transmission electron microscopy/doi:10.1016%2Fj.jim.2004.09.013/rivista:Journal of immunological methods (Print)/anno:2004/pagina_da:67/pagina_a:78/intervallo_pagine:67–78/volume:295

In this report, we have evaluated the effects of a TransFixR-based stabilisation technique on leukocyte scatter characteristics, immunophenotyping, membrane permeability, absolute cell counting and morphology to extend previously reported flow cytome

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3ddea06692e91875e003fad503de410
https://doi.org/10.1016/j.jim.2004.09.013

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Flow rate calibration II: A clinical evaluation study using PanLeucoGating as a single-platform protocol

Autor: Vivian Granger, David Barnett, Karen Goodfellow, John T. Reilly, Ian Storie, Liam Whitby, Alex Sawle

Publikováno v: Scopus-Elsevier

Background CD4+ T-lymphocyte enumeration is vital for monitoring disease progression in individuals positive for the human immunodeficiency virus (HIV), and as a result, there is a need to develop cost-effective protocols that provide accuracy, preci

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59cab9102dcd59cb00390cfd6c15473d
https://doi.org/10.1002/cyto.b.10050

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Perfect count: A novel approach for the single platform enumeration of absolute CD4+ T-lymphocytes

Autor: Janet Peel, Vivian Granger, Rosalie Ward, David Barnett, Liam Whitby, Theresa Smart, Karen Goodfellow, John T. Reilly, Alex Sawle, Ian Storie

Publikováno v: Cytometry. :47-52

Background: The derivation of reliable CD4 T lymphocyte counts is vital for the monitoring of disease progression and therapeutic effectiveness in HIV individuals. Flow cytometry has emerged as the method of choice for CD4 T lymphocyte enumeration, w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93a9fa2aed7b0bd6d6fb6df9c5129493
https://doi.org/10.1002/cyto.b.10065

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Reduction of variation in T-cell subset enumeration among 55 laboratories using single-platform, three or four-color flow cytometry based on CD45 and SSC-based gating of lymphocytes

Autor: Jaco Kraan, Michael Keeney, David Barnett, Jan-Willem Gratama, Vivian Granger

Publikováno v: Cytometry. 50:92-101

BACKGROUND Enumeration of CD4+ and CD8+ T-cell subsets provides relevant information for diagnosis and monitoring of patients with cellular immunodeficiencies. As a result, an external quality assurance scheme was implemented in Belgium, The Netherla

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ef0280d151d2a06838b5ae3a8e56601
https://doi.org/10.1002/cyto.10084

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Affordable CD4+ T cell counts by flow cytometry

Autor: David Barnett, llesh V Jani, Fred Mhalu, Debbie K. Glencross, George Janossy, Arabjan Iqbal, Gunnel Biberfeld, Vivian Granger, Lesley Scott, John T. Reilly, Eligius Lyamuya

Publikováno v: Journal of Immunological Methods. 257:145-154

We tested the feasibility and precision of affordable CD4+ T cell counting in resource-poor settings using a recently standardised fixative, TransFix in whole blood (WB) by flow cytometry (FCM). The precision of the assays was established under optim

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8396b1dec4ae59772140a09f38b2ed6c
https://doi.org/10.1016/s0022-1759(01)00458-6

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Low level leucocyte counting: a critical variable in the validation of leucodepleted blood transfusion components as highlighted by an external quality assessment study

Autor: David Barnett, Vivian Granger, J. Ginnever, Karen Goodfellow, John T. Reilly, Liam Whitby

Publikováno v: Clinical & Laboratory Haematology. 23:43-51

Summary Leucocyte counts of < 5 · 10 6 per blood transfusion product are currently recommended in the UK in order to reduce transfusion-related infections and febrile reactions. Routine leucocyte depletion, however, requires the development of relia

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f241c219e9897ffa4a21c3f694f0b7a6
https://doi.org/10.1046/j.1365-2257.2001.00356.x

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Standardization of lymphocyte antibody binding capacity - a multi-centre study

Autor: B.K. Shenton, Ian Storie, Stephen J. Richards, John T. Reilly, David Barnett, Vivian Granger, J. Lawry, Liam Whitby, S. Garner, S. Brough, A.E. Bell

Publikováno v: Clinical & Laboratory Haematology. 22:89-96

As quantitative flow cytometry is being increasingly used to characterize non-malignant and malignant disorders, interlaboratory standardization becomes an important issue. However, the lack of standardized methods and process controls with predefine

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1aeb7e9b52c571150c4706bd32951d1c
https://doi.org/10.1046/j.1365-2257.2000.00286.x

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Reduction of intra- and interlaboratory variation in CD34+ stem cell enumeration using stable test material, standard protocols and targeted training

Autor: Stefano Papa, Liam Whitby, Jaco Kraan, Vivian Granger, David Barnett, John T. Reilly, Jan-Willem Gratama

Publikováno v: British Journal of Haematology. 108:784-792

The European Working Group on Clinical Cell Analysis (EWGCCA) has, in preparation for a multicentre peripheral blood stem cell clinical trial, developed a single-platform flow cytometric protocol for the enumeration of CD34+ stem cells. Using this pr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ca090be1aec0f925df2e7af4b97a1270
https://doi.org/10.1046/j.1365-2141.2000.01932.x

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Absolute CD4+ T-lymphocyte and CD34+ stem cell counts by single-platform flow cytometry: the way forward

Autor: John T. Reilly, David Barnett, Vivian Granger, Ian Storie, Liam Whitby

Publikováno v: British Journal of Haematology. 106:1059-1062

To determine the potential advantage of single-platform technology in the enumeration of CD4+ T lymphocyte and CD34+ stem cells, data has been analysed from the UK NEQAS for Leucocyte Immunophenotyping schemes. The inter-laboratory CVs for CD4+ T lym

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fef29043d7551e4584499eb180e496d0
https://doi.org/10.1046/j.1365-2141.1999.01632.x

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