Chemical stability of a cold-active cellulase with high tolerance toward surfactants and chaotropic agent

Autor: Thaís V. Souza, Juscemácia N. Araujo, Viviam M. da Silva, Marcelo V. Liberato, Agnes C. Pimentel, Thabata M. Alvarez, Fabio M. Squina, Wanius Garcia

Publikováno v: Biotechnology Reports, Vol 9, Iss C, Pp 1-8 (2016)

CelE1 is a cold-active endo-acting glucanase with high activity at a broad temperature range and under alkaline conditions. Here, we examined the effects of pH on the secondary and tertiary structures, net charge, and activity of CelE1. Although vari

Externí odkaz: https://doaj.org/article/381d2b7a6c1f4af88b0501e055fd15a4

Conformational changes in a hyperthermostable glycoside hydrolase: enzymatic activity is a consequence of the loop dynamics and protonation balance.

Autor: Leandro C de Oliveira, Viviam M da Silva, Francieli Colussi, Aline D Cabral, Mario de Oliveira Neto, Fabio M Squina, Wanius Garcia

Publikováno v: PLoS ONE, Vol 10, Iss 2, p e0118225 (2015)

Endo-β-1, 4-mannanase from Thermotoga petrophila (TpMan) is a modular hyperthermostable enzyme involved in the degradation of mannan-containing polysaccharides. The degradation of these polysaccharides represents a key step for several industrial ap

Externí odkaz: https://doaj.org/article/b03ae7ccf5c64045a94462b2c186e011

High-resolution structure of a modular hyperthermostable endo-β-1,4-mannanase from Thermotoga petrophila: the ancillary immunoglobulin-like module is a thermostabilizing domain

Autor: Yvain Nicolet, Márcia Aparecida Sperança, Aline Diniz Cabral, João R. C. Muniz, Fabio M. Squina, Wanius Garcia, Lydie Martin, Viviam M. da Silva

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Biochimica et Biophysica Acta Proteins and Proteomics
Biochimica et Biophysica Acta Proteins and Proteomics, Elsevier, 2020, 1868 (8), pp.140437. ⟨10.1016/j.bbapap.2020.140437⟩
Biochimica et Biophysica Acta Proteins and Proteomics, 2020, 1868 (8), pp.140437. ⟨10.1016/j.bbapap.2020.140437⟩

International audience; The endo-β-1,4-mannanase from the hyperthermostable bacterium Thermotoga petrophila (TpMan) is an enzyme that catalyzes the hydrolysis of mannan and heteromannan polysaccharides. Of the three domains that comprise TpMan, the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3565a2d31995a09a466c6799cbfe3fe3

Nucleoprotein from the unique human infecting orthobunyavirus of simbu serogroup (Oropouche virus) forms higher order oligomers in complex with nucleic acids in vitro

Autor: Leandro F. Estrozi, Daphna Fenel, Juliana Londoño Murillo, Juliete Vitorino dos Santos, Márcia Aparecida Sperança, João R. C. Muniz, Aline Diniz Cabral, Mabel Uehara, Viviam M. da Silva, Wanius Garcia

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Amino Acids
Amino Acids, Springer Verlag, 2018, 50 (6), pp.711-721. ⟨10.1007/s00726-018-2560-4⟩
Amino Acids, 2018, 50 (6), pp.711-721. ⟨10.1007/s00726-018-2560-4⟩

International audience; Oropouche virus (OROV) is the unique known human pathogen belonging to serogroup Simbu of Orthobunyavirus genus and Bunyaviridae family. OROV is transmitted by wild mosquitoes species to sloths, rodents, monkeys and birds in s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8cebe9afe886616eb03204effe266faf

Photobiosynthesis of stable and functional silver/silver chloride nanoparticles with hydrolytic activity using hyperthermophilic β-glucosidases with industrial potential

Autor: Aryane Tofanello, Fabio M. Squina, Juliana A. P. Sato, Viviam M. da Silva, Juscemácia N. Araújo, Wanius Garcia, Iseli L. Nantes

Publikováno v: International journal of biological macromolecules. 102

The β-glucosidases are important enzymes employed in a large number of processes and industrial applications, including biofuel production from biomass. Therefore, in this study, we reported for the first time the photobiosynthesis of stable and fun

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4197478d9e3d3d16eb9dedba39cf6d7f
https://pubmed.ncbi.nlm.nih.gov/28400186