Zobrazeno 1 - 10
of 42
pro vyhledávání: '"Vitor B Pinheiro"'
Publikováno v:
Frontiers in Molecular Biosciences, Vol 11 (2024)
Directed evolution is a powerful tool that can bypass gaps in our understanding of the sequence-function relationship of proteins and still isolate variants with desired activities, properties, and substrate specificities. The rise of directed evolut
Externí odkaz:
https://doaj.org/article/c1bc1e805d0a446b9d89e7f53b9ac9c8
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
Cancer is a leading cause of mortality globally, often diagnosed at advanced stages with metastases already present, complicating treatment efficacy. Traditional treatments like chemotherapy and radiotherapy face challenges such as lack of specificit
Externí odkaz:
https://doaj.org/article/cc13b65a926a4caa85e972e1454ebcb1
Publikováno v:
PLoS ONE, Vol 8, Iss 8, p e70892 (2013)
Thermophilic DNA polymerases of the polB family are of great importance in biotechnological applications including high-fidelity PCR. Of particular interest is the relative promiscuity of engineered versions of the exo- form of polymerases from the T
Externí odkaz:
https://doaj.org/article/daad31c361ba4c1d978eee1c78aa59e2
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Abstract Insertions and deletions (indels) are known to affect function, biophysical properties and substrate specificity of enzymes, and they play a central role in evolution. Despite such clear significance, this class of mutation remains an undere
Externí odkaz:
https://doaj.org/article/c189ef69ca0b404eb475c4a61034aca1
Autor:
Alex J.H. Fedorec, Tanel Ozdemir, Anjali Doshi, Yan-Kay Ho, Luca Rosa, Jack Rutter, Oscar Velazquez, Vitor B. Pinheiro, Tal Danino, Chris P. Barnes
Publikováno v:
iScience, Vol 14, Iss , Pp 323-334 (2019)
Summary: Plasmids are the workhorse of both industrial biotechnology and synthetic biology, but ensuring they remain in bacterial cells is a challenge. Antibiotic selection cannot be used to stabilize plasmids in most real-world applications, and ins
Externí odkaz:
https://doaj.org/article/7fc97d305d384cc1b919e95418a68bcc
Autor:
Camille Samson, Pierre Legrand, Mustafa Tekpinar, Jef Rozenski, Mikhail Abramov, Philipp Holliger, Vitor B. Pinheiro, Piet Herdewijn, Marc Delarue
Publikováno v:
Biomolecules, Vol 10, Iss 12, p 1647 (2020)
Archaeal DNA polymerases from the B-family (polB) have found essential applications in biotechnology. In addition, some of their variants can accept a wide range of modified nucleotides or xenobiotic nucleotides, such as 1,5-anhydrohexitol nucleic ac
Externí odkaz:
https://doaj.org/article/b095ac31ee974895994ea5dcc61f0c3a
Detailed biochemical characterization of natural and mutant enzymes provides essential clues to understand their mechanisms. There are, however, limits to the throughput of such approaches and they are not without errors. DNA polymerases have benefit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::60f924c7fd6f92beb2050602c6f135d2
https://doi.org/10.1101/2022.11.08.515583
https://doi.org/10.1101/2022.11.08.515583
Autor:
Charlotte C. van de Velde, Clémence Joseph, Anaïs Biclot, Geert R. B. Huys, Vitor B. Pinheiro, Kristel Bernaerts, Jeroen Raes, Karoline Faust
Publikováno v:
ISME Communications. 2
A bottleneck for microbial community experiments with many samples and/or replicates is the fast quantification of individual taxon abundances, which is commonly achieved through sequencing marker genes such as the 16S rRNA gene. Here, we propose a n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0772d057ae29601188100c717b5a1293
https://doi.org/10.1038/s43705-022-00123-6
https://doi.org/10.1038/s43705-022-00123-6
Autor:
Fernando R. Caires, Samuel R. Silva, Marcos Veríssimo-Alves, Vitor B. Pinheiro, Rinaldo W. Montalvão
MotivationModern AI-based tools are increasing the number of protein structures available, creating an opportunity and a challenge for automated high-throughput drug discovery pipelines. The amount of data is overwhelming for the current methods, thu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7b221106ba31dfb57afda96f15bec969
https://doi.org/10.1101/2022.01.19.476747
https://doi.org/10.1101/2022.01.19.476747
Autor:
Vitor B. Pinheiro, Warren Hazelton, Eleftheria-Pinelopi Stamou, Santiago Chaillou, Leticia L. Torres, Ana B. Riesco
Plasmids of the ColE1 family are among the most frequently used plasmids in molecular biology. They were adopted early in the field for many biotechnology applications, and as model systems to study plasmid biology. The mechanism of replication of Co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f62443f36a30e3768ae33c574cc81f4e
https://doi.org/10.1101/2021.11.25.470029
https://doi.org/10.1101/2021.11.25.470029