Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Vitali Borisenko"'
Autor:
Danlin Zhen, Vitali Borisenko, Katherine E. Brechun, Wouter D. Hoff, Andrew Woolley, Masato Kumauchi, Katja M. Arndt, Anna S. I. Jaikaran
Publikováno v:
Biochemistry. 58:2682-2694
We report the design and characterization of photoactive yellow protein (PYP)-blue fluorescent protein (mTagBFP) fusion constructs that permit the direct assay of reconstitution and function of the PYP domain. These constructs allow for in vivo testi
Autor:
M. Cynthia Goh, Vitali Borisenko
Publikováno v:
Analytical Methods. 9:2392-2396
We present a simple and inexpensive method for enabling bacterial cell capture in a 2D array of traps, such that their growth can be monitored under controlled dynamic environments through diffractive optics integrated into a microfluidic chip. As an
Autor:
Vitali Borisenko, G. Andrew Woolley
Publikováno v:
Journal of Photochemistry and Photobiology A: Chemistry. 173:21-28
Reversible photo-control of protein structure and function is used by Nature to direct complex biological response to light. Reversibility is a feature that has been difficult to reliably achieve in analogous biomimetic systems. We report here measur
Autor:
G. A. Woolley, Tyler Lougheed, Jan C. Behrends, Niels Fertig, E. Füreder-Kitzmüller, Jan Hesse, Vitali Borisenko, Gerhard J. Schütz
Publikováno v:
Scopus-Elsevier
We report here an approach for simultaneous fluorescence imaging and electrical recording of single ion channels in planar bilayer membranes. As a test case, fluorescently labeled (Cy3 and Cy5) gramicidin derivatives were imaged at the single-molecul
Autor:
G. Andrew Woolley, Nikolay S. Melik-Nubarov, Elena A. Kotova, Yuri N. Antonenko, Vitali Borisenko
Publikováno v:
Biophysical Journal. 82:1308-1318
The effects of different anionic polymers on the kinetic properties of ionic channels formed by neutral gramicidin A (gA) and its positively charged analogs gramicidin-tris(2-aminoethyl)amine (gram-TAEA) and gramicidin-ethylenediamine (gram-EDA) in a
Publikováno v:
Bioconjugate Chemistry. 12:594-602
Single-molecule spectroscopies in combination with single-channel patch-clamp measurements have the potential for providing new information on ion channel gating processes. Fluorescent gramicidin derivatives could provide a means for calibrating such
Publikováno v:
Journal of the American Chemical Society. 122:6364-6370
Optical control of ion channel gating could permit the functional manipulation of excitable cells. We wished to examine the feasibility of using optical switching of ion−dipole interactions as a me...
Publikováno v:
Scopus-Elsevier
A dimeric alamethicin analog with lysine at position 18 in the sequence (alm-K18) was previously shown to form stable anion-selective channels in membranes at pH 7.0 [Starostin, A. V., R. Butan, V. Borisenko, D. A. James, H. Wenschuh, M. S. Sansom, a
Publikováno v:
Clinical Chemistry. 52:2168-2170
There is an increasing need for high-sensitivity immunoassays that can be used in point-of-care patient testing of complex media. For example, analytes such as the natriuretic peptides and recently discovered sepsis markers are found in blood in very
Publikováno v:
Organicbiomolecular chemistry. 2(19)
Incorporation of photo-switchable amino acids into peptides and proteins offers prospects for the control of complex biochemical processes using light. Currently, only a few photo-switchable amino acids are known. We report the design and synthesis o