Výsledky vyhledávání - "Vishal Nashine"

A Fluorescence-Based High-Throughput Coupled Enzymatic Assay for Quantitation of Isoaspartate in Proteins and Peptides

Autor: Vishal Nashine, Monica L. Adams, Ajit S. Narang, Rajesh B. Gandhi, Yong Quan, Aastha Puri

Publikováno v: AAPS PharmSciTech. 18:803-808

Formation of isoaspartate (IsoAsp) from spontaneous asparagine (Asn) deamidation or aspartate (Asp) isomerization is one of the most common non-enzymatic pathways of chemical degradation of protein and peptide pharmaceuticals. Rapid quantitation of I

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::988a482d9303bde13f56472e46051c91
https://doi.org/10.1208/s12249-016-0570-7

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Evaluation of excipients to optimize cell therapy cryopreservation using a definitive screening design

Autor: Devon Zimmerman, Sagar Shelake, Bharathikumar Vellalore Maruthachalam, Maria Ozerova, Amos Tsai, Vishal Nashine

Publikováno v: Cryobiology. 97:277

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::42b06a1868ad4ae46c7bd628c609ad23
https://doi.org/10.1016/j.cryobiol.2020.10.110

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Utilization of Zwitterion-Based Solutions to Dissect the Relative Effects of Solution pH and Ionic Strength on the Aggregation Behavior and Conformational Stability of a Fusion Protein

Autor: Rajesh B. Gandhi, Monica L. Adams, Vishal Nashine, Thomas Palm, Rong Zhou

Publikováno v: Journal of Pharmaceutical Sciences. 103:3065-3074

Solution pH and ionic strength (I) have complex effects on protein stability. We developed an experimental approach based on exploitation of the zwitterionic characteristic of amino acid molecules to probe the relative contribution from each. A varie

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b848b136f0ee550da7b72b6959240ed3
https://doi.org/10.1002/jps.24118

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Significance of Unfolding Thermodynamics for Predicting Aggregation Kinetics: A Case Study on High Concentration Solutions of a Multi-Domain Protein

Autor: Venkatramana M. Rao, Dahlheim Charles E, Andrew M. Kroetsch, Vikram Sadineni, Atul Saluja, Vishal Nashine, Amol Mungikar

Publikováno v: Pharmaceutical Research. 31:1575-1587

To enable aggregation rate prediction over a broad temperature range for complex multi-domain proteins at high concentrations. Thermal unfolding, non-isothermal kinetics and storage stability studies were conducted on a model multi-domain protein (MD

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14fa734e801842e3c3fc78256f9c18bb
https://doi.org/10.1007/s11095-013-1263-5

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Orthogonal High-Throughput Thermal Scanning Method for Rank Ordering Protein Formulations

Autor: Rong Zhou, Vishal Nashine, Monica L. Adams, Erinc Sahin, Andrew M. Kroetsch

Publikováno v: AAPS PharmSciTech. 14:1360-1366

A high-throughput thermal-scanning method to rank-order formulation conditions for therapeutic proteins is described. Apparent transition temperatures for unfolding and aggregation of four different proteins are determined using the dyes SYPRO Orange

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4eed70f5aff9f73b1d97cdd1bf66819e
https://doi.org/10.1208/s12249-013-0026-2

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A Small-Scale, Material-Saving Approach to Rank-Order Lyophilized Formulations Based on Reconstitution Time

Autor: Vishal Nashine, Mary Morcos, Chandran Sachin, Atul Saluja

Publikováno v: Journal of Pharmaceutical Innovation. 8:187-194

The goal of this manuscript is to describe a system amenable to automation that could estimate reconstitution times, and thus rank-order lyophilized systems using a small-scale, miniaturized, plate-based approach. Multiple formulations of proprietary

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a8cd6bf30550fc7771fdff088ac65bea
https://doi.org/10.1007/s12247-013-9158-x

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A High-Throughput Bioluminescent Assay to Monitor the Deamidation of Asparagine and Isomerization of Aspartate Residues in Therapeutic Proteins and Antibodies

Autor: Vishal Nashine, Kevin Hsiao, Monica L. Adams, Rushikesh K. Patel, Juliano Alves, Said A. Goueli

Publikováno v: Journal of pharmaceutical sciences. 106(6)

Since the introduction of Herceptin and Rituximab in 1986, therapeutic antibodies have gained tremendous momentum in the treatment of broad range of several diseases such as cancer and inflammation. Selection of the clinical candidate mAb usually sta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4fbc35fc796ab6ecd6464a934afe664
https://pubmed.ncbi.nlm.nih.gov/28263844

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Abstract 5434: A high through-put bioluminescent assay to monitor the deamidation of asparagine and isomerization of aspartate residues in therapeutic proteins and antibodies

Autor: Vishal Nashine, Said A. Goueli, Rushikesh K. Patel, Kevin Hsiao

Publikováno v: Cancer Research. 75:5434-5434

Spontaneous degradation reactions such as oxidation, glycation, deamidation, Isomerization, and racemization are non-enzymatic modifications and produce functionally damaged species reflecting aging effect at the molecular level. L-asparagine (Asn) a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::889abd301756c9033e9652ecfeef56f6
https://doi.org/10.1158/1538-7445.am2015-5434

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