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A Bioluminescence Resonance Energy Transfer–Based Reporter System: Characterization and Applications
Autor:
Vincent Moeschl, Knut Stieger, Christian Thiel, Philip Kleekamp, Dileep Bonthu, Matthew Ellinwood, Tobias Wimmer, Lyubomyr Lytvynchuk
Publikováno v:
The CRISPR Journal. 4:884-895
Genome editing strategies and DNA repair research need powerful analytical tools. We generated a bioluminescence resonance energy transfer (BRET)-based reporter for the quantification of indel frequencies induced by DNA repair. The BRET reporter, exp