Measuring S-Phase Duration from Asynchronous Cells Using Dual EdU-BrdU Pulse-Chase Labeling Flow Cytometry

Autor: Marta Bialic, Baraah Al Ahmad Nachar, Maria Koźlak, Vincent Coulon, Etienne Schwob

Publikováno v: Genes; Volume 13; Issue 3; Pages: 408

Eukaryotes duplicate their chromosomes during the cell cycle S phase using thousands of initiation sites, tunable fork speed and megabase-long spatio-temporal replication programs. The duration of S phase is fairly constant within a given cell type,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22a0542a4a6e59216de2233fdaacedf2
https://doi.org/10.3390/genes13030408

A mitosis block links active cell cycle with human epidermal differentiation and results in endoreplication.

Autor: Jennifer Zanet, Ana Freije, María Ruiz, Vincent Coulon, J Ramón Sanz, Jean Chiesa, Alberto Gandarillas

Publikováno v: PLoS ONE, Vol 5, Iss 12, p e15701 (2010)

How human self-renewal tissues co-ordinate proliferation with differentiation is unclear. Human epidermis undergoes continuous cell growth and differentiation and is permanently exposed to mutagenic hazard. Keratinocytes are thought to arrest cell gr

Externí odkaz: https://doaj.org/article/b2e16f1831934bf18ccd4a6e4f286b5b

Inefficient differentiation response to cell cycle stress leads to genomic instability and malignant progression of squamous carcinoma cells

Autor: Laura Sanz Ceballos, Carmen Segrelles, Ana García-Valtuille, Rut Molinuevo, Ramon M. Pujol, Vincent Coulon, Agustí Toll, José M Bernal, Isabel de Pedro, Daniel López-Aventín, Ana Freije, Corina Lorz, Pilar Alonso-Lecue, Jesús M. Paramio, J Ramón Sanz, Alberto Gandarillas, Francisco Mazorra

Publikováno v: Cell Death & Disease
Cell Death and Disease
Cell Death and Disease, Nature Publishing Group, 2017, 8 (6), pp.e2901. ⟨10.1038/cddis.2017.259⟩

Squamous cell carcinoma (SCC) or epidermoid cancer is a frequent and aggressive malignancy. However in apparent paradox it retains the squamous differentiation phenotype except for very dysplastic lesions. We have shown that cell cycle stress in norm

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82fde530f9345512d3da778cc9ac35ca
https://pubmed.ncbi.nlm.nih.gov/28661481

Analyzing the dynamics of DNA replication in Mammalian cells using DNA combing

Autor: Marta, Bialic, Vincent, Coulon, Marjorie, Drac, Thierry, Gostan, Etienne, Schwob

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1300

How cells duplicate their chromosomes is a key determinant of cell identity and genome stability. DNA replication can initiate from more than 100,000 sites distributed along mammalian chromosomes, yet a given cell uses only a subset of these origins

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1eebaf166f9425653142321d7b194a91
https://pubmed.ncbi.nlm.nih.gov/25916705

Decreased MCM2-6 in Drosophila S2 cells does not generate significant DNA damage or cause a marked increase in sensitivity to replication interference

Autor: Christelle de Renty, Gilles Crevel, Vincent Coulon, Isabelle Crevel, Sue Cotterill, Thierry Gostan

Publikováno v: PLoS ONE, Vol 6, Iss 11, p e27101 (2011)
PLoS ONE
PLoS ONE, Public Library of Science, 2011, 6 (11), pp.e27101. ⟨10.1371/journal.pone.0027101⟩

International audience; A reduction in the level of some MCM proteins in human cancer cells (MCM5 in U20S cells or MCM3 in Hela cells) causes a rapid increase in the level of DNA damage under normal conditions of cell proliferation and a loss of viab

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9724c4805ce6e489cddfb967cb55629e
http://europepmc.org/articles/PMC3216938?pdf=render