Výsledky vyhledávání - "Vinay K Nagarajan"

RNA degradome analysis reveals DNE1 endoribonuclease is required for the turnover of diverse mRNA substrates in Arabidopsis

Autor: Vinay K Nagarajan, Catherine J Stuart, Anna T DiBattista, Monica Accerbi, Jeffrey L Caplan, Pamela J Green

Publikováno v: The Plant Cell. 35:1936-1955

In plants, cytoplasmic mRNA decay is critical for posttranscriptionally controlling gene expression and for maintaining cellular RNA homeostasis. Arabidopsis DCP1-ASSOCIATED NYN ENDORIBONUCLEASE 1 (DNE1) is a cytoplasmic mRNA decay factor that intera

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5db85455cb662eb0c2bceb3c9a38b694
https://doi.org/10.1093/plcell/koad085

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Comparative parallel analysis of RNA ends identifies mRNA substrates of a tRNA splicing endonuclease-initiated mRNA decay pathway

Autor: Tao Li, Jennifer E. Hurtig, Ambro van Hoof, Ti Chun Chao, Kuang-Lei Tsai, Michelle A. Steiger, Vinay K. Nagarajan

Publikováno v: Proc Natl Acad Sci U S A

Eukaryotes share a conserved messenger RNA (mRNA) decay pathway in which bulk mRNA is degraded by exoribonucleases. In addition, it has become clear that more specialized mRNA decay pathways are initiated by endonucleolytic cleavage at particular sit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba8799f782d2f04f9e964cc35b20ce67
https://pubmed.ncbi.nlm.nih.gov/33649230

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RNA degradomes reveal substrates and importance for dark and nitrogen stress responses of Arabidopsis XRN4

Autor: Pamela J. Green, Patrick M Kukulich, Bryan von Hagel, Vinay K. Nagarajan

Publikováno v: Nucleic Acids Research

XRN4, the plant cytoplasmic homolog of yeast and metazoan XRN1, catalyzes exoribonucleolytic degradation of uncapped mRNAs from the 5′ end. Most studies of cytoplasmic XRN substrates have focused on polyadenylated transcripts, although many substra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::349ba9ce30591892a22f3d1f8fac43d1
https://doi.org/10.1093/nar/gkz712

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Efforts to transform Heterosigma akashiwo using an Agrobacterium-mediatedapproach v4

Autor: Vinay K Nagarajan, Monica Accerbi, Pamela J Green

This protocol was developed for transformation of Heterosigma akashiwo byAgrobacterium tumefaciens.H. akashiwodoes not grow on solid medium, so transformants are grown "in bulk" and should not be considered clonal. We have had limited success with th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5aa5975192fee8eb4bb1559e30dcd98c
https://doi.org/10.17504/protocols.io.4ytgxwn

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Efforts to transform Heterosigma akashiwo using an Agrobacterium-mediatedapproach v2

Autor: Vinay K Nagarajan, Monica Accerbi, Pamela J Green

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c29088a4748e0b7234645b1246f8a650
https://doi.org/10.17504/protocols.io.xjxfkpn

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Arabidopsis MYB-Related HHO2 Exerts a Regulatory Influence on a Subset of Root Traits and Genes Governing Phosphate Homeostasis

Autor: Vinay K. Nagarajan, Kashchandra G. Raghothama, Michael D. Poling, Ajay Jain, Viswanathan Satheesh

Publikováno v: Plant and Cell Physiology. 57:1142-1152

Phosphate (Pi), an essential macronutrient required for growth and development of plants, is often limiting in soils. Pi deficiency modulates the expression of Pi starvation-responsive (PSR) genes including transcription factors (TFs). Here, we eluci

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5265a7766e0856a7d351c054ade74fae
https://doi.org/10.1093/pcp/pcw063

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Efforts to transform Heterosigma akashiwo using an Agrobacterium-mediatedapproach v1

Autor: Vinay K Nagarajan, Monica Accerbi, Pamela J Green

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::547916ab349f790442f1fdde3854674a
https://doi.org/10.17504/protocols.io.jsxcnfn

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Modified genomic DNA extraction method for Heterosigma akashiwo v1

Autor: Monica Accerbi, Vinay K Nagarajan, Kathryn Coyne, Pamela J Green

This protocol was developed for isolating high quality genomic DNA from Heterosigma akashiwo for the purpose of Next generation sequencing technologies. We successfully repeated this protocol to obtain genomic DNA.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5614681cc17f1869655e9152d9a33ec8
https://doi.org/10.17504/protocols.io.himb4c6

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Modified total RNA extraction for Heterosigma akashiwo using the Qiagen RNeasy kit v1

Autor: Monica Accerbi, Vinay K Nagarajan, Kathryn Coyne, Pamela J Green

This protocol isa modifiedversion of the Qiagen RNeasy assay for isolating total RNA from Heterosigma akashiwo.We used this protocol to obtain high quality RNA for downstream analysis such as quantitative PCR.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3f342db8e895c95698438089e897ee32
https://doi.org/10.17504/protocols.io.hipb4dn

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