Zobrazeno 1 - 10
of 35
pro vyhledávání: '"Viktor Menart"'
Publikováno v:
Food Technology and Biotechnology, Vol 41, Iss 4, Pp 345-351 (2003)
The most common expression strategy for secreting heterologous proteins from filamentous fungus Aspergillus niger is based on fusion with glucoamylase gene which contains cleavage site for kexin protease (KEX2). However, secretion of recombinant prot
Externí odkaz:
https://doaj.org/article/a418de7300d04869a8dd208072c90916
Publikováno v:
The Scientific World Journal, Vol 2, Pp 1312-1325 (2002)
When studying two different histidine tags attached to the N-termini of the trimeric cytokine tumor necrosis factor alpha (TNF), the biological activity — measured as cytotoxicity on the L-929 cell line — of both tagged proteins was drastically r
Externí odkaz:
https://doaj.org/article/9bccfef6285b40ea88ac8fa7fca4708d
Autor:
Simona Jevševar, Vladka Gaberc-Porekar, Anton Stalc, Mojca Pernus, Antony Meager, Viktor Menart
Publikováno v:
Pflugers Archiv : European journal of physiology. 439(Suppl 1)
The first essential step in TNF signal transduction is believed to be clustering of the membrane bound receptors around the trimeric TNF molecule. To check if one receptor binding site would be enough to trigger the signal, we tried to prepare severa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e466bb9c2660a130a6e5147bcb8c106c
https://pubmed.ncbi.nlm.nih.gov/28176092
https://pubmed.ncbi.nlm.nih.gov/28176092
Autor:
Simona Jevsevar, Jože Grdadolnik, Barbara Podobnik, Vladka Gaberc-Porekar, Irena Fonda, Viktor Menart
Publikováno v:
Biotechnology Progress. 21:632-639
Human granulocyte-colony stimulating factor (hG-CSF), an important biopharmaceutical drug used in oncology, is currently produced mainly in Escherichia coli. Expression of human hG-CSF gene in E. coli is very low, and therefore a semisynthetic, codon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c60e44de60915ff193117ac346c9e2c
https://doi.org/10.1021/bp0497839
https://doi.org/10.1021/bp0497839
Publikováno v:
Journal of Chromatography B. 867:119-125
The N-terminus of the trimeric TNF-alpha molecule comprises two basic arginines within the short amino-acid sequence VRSSSR, which is here shown to be essential for binding of TNF-alpha to heparin-Sepharose. Mixed trimers containing full-length and D
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4a236ab9a28213cf6af595992a12ca9
https://doi.org/10.1016/j.jchromb.2008.03.023
https://doi.org/10.1016/j.jchromb.2008.03.023
Autor:
Viktor Menart, Vladka Gaberc-Porekar
Publikováno v:
Chemical Engineering & Technology. 28:1306-1314
Attachment of oligo-histidine tag (His-tag) to the protein N- or C-terminus is a good example of early and successful protein engineering to design a unique and generalized purification scheme for virtually any protein. Thus relatively strong and spe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e21b2e47377e1cb3177226d9faf8b2ad
https://doi.org/10.1002/ceat.200500167
https://doi.org/10.1002/ceat.200500167
Publikováno v:
Journal of Biotechnology. 109:115-122
Wild type gene for green fluorescent protein (GFP) was stably integrated into the Pichia pastoris genome and yielded an expression level of over 40% of total cellular protein. The high cytoplasmic concentration of fluorescent (properly folded and pro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da98a426fcb9103dd0f388d5e3331d97
https://doi.org/10.1016/j.jbiotec.2003.11.013
https://doi.org/10.1016/j.jbiotec.2003.11.013
Publikováno v:
Biotechnology and Bioengineering. 83:181-190
Constitutive and thermoinducible expression plasmids based on strong PR,PL promoters from phage lambda were compared for production of TNF-alpha and its analogs under various conditions. Much higher accumulation of TNF was obtained in a constitutive
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c6f1d64cc08f97199eadfe47a1e41d8
https://doi.org/10.1002/bit.10660
https://doi.org/10.1002/bit.10660
Publikováno v:
Food Technology and Biotechnology, Vol 41, Iss 4, Pp 345-351 (2003)
Food Technology and Biotechnology
Volume 41
Issue 4
Food Technology and Biotechnology
Volume 41
Issue 4
The most common expression strategy for secreting heterologous proteins from filamentous fungus Aspergillus niger is based on fusion with glucoamylase gene which contains cleavage site for kexin protease (KEX2). However, secretion of recombinant prot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::3d784860905c2dad24941d9e719769e7
http://hrcak.srce.hr/file/185064
http://hrcak.srce.hr/file/185064
Publikováno v:
Annals of the New York Academy of Sciences. 973:194-206
Our tumor necrosis factor-alpha (TNF-alpha) analog LK-805 (E107K) exhibited twofold higher specific cytotoxicity on the mouse fibroblast L-929 cell line than its native counterpart. In addition, significantly lowered systemic toxicity was observed in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2569605cf4b8c5b46f4172e708371eb1
https://doi.org/10.1111/j.1749-6632.2002.tb04632.x
https://doi.org/10.1111/j.1749-6632.2002.tb04632.x