Increased mAb production in amplified CHO cell lines is associated with increased interaction of CREB1 with transgene promoter

Autor: Hussain Dahodwala, Prashant Kaushik, Vijay Tejwani, Chih-Chung Kuo, Patrice Menard, Michael Henry, Bjorn G. Voldborg, Nathan E. Lewis, Paula Meleady, Susan T. Sharfstein

Publikováno v: Current Research in Biotechnology, Vol 1, Iss , Pp 49-57 (2019)

Most therapeutic monoclonal antibodies in biopharmaceutical processes are produced in Chinese hamster ovary (CHO) cells. Technological advances have rendered the selection procedure for higher producers a robust protocol. However, information on mole

Externí odkaz: https://doaj.org/article/effda2b4c08944dba65439c8d3ad4494

High‐throughput and automation advances for accelerating single‐cell cloning, monoclonality and early phase clone screening steps in mammalian cell line development for biologics production

Autor: Vijay Tejwani, Susan T. Sharfstein, Minal Chaudhari, Toyaj Rai

Publikováno v: Biotechnology Progress. 37

Mammalian cell line development is a multistep process wherein timelines for developing clonal cells to be used as manufacturing cell lines for biologics production can commonly extend to 9 months when no automation or modern molecular technologies a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27a347717953bdca5c523322c8274783
https://doi.org/10.1002/btpr.3208

Proteogenomic Annotation of Chinese Hamsters Reveals Extensive Novel Translation Events and Endogenous Retroviral Elements

Autor: Nathan E. Lewis, Michael A. Bowen, Paula Meleady, Seong Won Cha, Shangzhong Li, Deniz Baycin Hizal, Susan T. Sharfstein, Prashant Kaushik, Vijay Tejwani, Robert N. Cole, Michael J. Betenbaugh, Kelley M. Heffner, Michael Henry, Raghothama Chaerkady, Vineet Bafna

Publikováno v: J Proteome Res

A high quality genome annotation greatly facilitates successful cell line engineering. Standard draft genome annotation pipelines are based largely onde novogene prediction, homology, and RNA-Seq data. However, draft annotations can suffer from incor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::530ec4e0c70812cf31344fba75aa1e61
https://doi.org/10.1021/acs.jproteome.8b00935

Investigation of the NADH/NAD + ratio in Ralstonia eutropha using the fluorescence reporter protein Peredox

Autor: Vijay Tejwani, Ingo Zebger, Thomas Friedrich, Marius Horch, Svea Wilkening, Franz-Josef Schmitt, Oliver Lenz

Publikováno v: Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1858:86-94

Ralstonia eutropha is a hydrogen-oxidizing ("Knallgas") bacterium that can easily switch between heterotrophic and autotrophic metabolism to thrive in aerobic and anaerobic environments. Its versatile metabolism makes R. eutropha an attractive host f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ffc7498deba072882467dd5e0e2b218e
https://doi.org/10.1016/j.bbabio.2016.11.001

Glycoengineering in CHO Cells: Advances in Systems Biology

Autor: Vijay Tejwani, Jong Hyun Nam, Mikael Rørdam Andersen, Susan T. Sharfstein

Publikováno v: Tejwani, V, Andersen, M R, Nam, J H & Sharfstein, S T 2018, ' Glycoengineering in CHO cells: Advances in systems biology ', Biotechnology Journal, vol. 13, no. 3, 1700234 . https://doi.org/10.1002/biot.201700234

For several decades, glycoprotein biologics have been successfully produced from Chinese hamster ovary (CHO) cells. The therapeutic efficacy and potency of glycoprotein biologics are often dictated by their post-translational modifications, particula

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6adbccd3a54f7c5ed9002e20cc1d8de3
https://doi.org/10.1002/biot.201700234