Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Victoria L. Singer"'
Autor:
W. Gregory Cox, Victoria L. Singer
Publikováno v:
BioTechniques, Vol 36, Iss 1, Pp 114-122 (2004)
Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye. Alternatively, 5-(3-aminoallyl)-2′-deox
Externí odkaz:
https://doaj.org/article/d77e665a74d64608978ca39be44a45ce
Publikováno v:
BioTechniques, Vol 34, Iss 4, Pp 850-861 (2003)
We developed a sensitive fluorescence assay for the quantitation of proteins in solution using the NanoOrange® reagent, a merocyanine dye that produces a large increase in fluorescence quantum yield upon interaction with detergent-coated proteins. T
Externí odkaz:
https://doaj.org/article/fc90fdd151f54a96b5bb64ca7780f047
Autor:
Victoria L. Singer, Laurie J. Jones
Publikováno v:
Analytical Biochemistry. 293:8-15
We have developed a simple, sensitive, fluorescence microplate-based assay for tumor necrosis factor (TNF) biological activity. The assay employs SYTOX Green nucleic acid stain to detect TNF-induced cell necrosis in actinomycin D sensitized cultured
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::673d7c2d215240038570e1e029e3b006
https://doi.org/10.1006/abio.2001.5116
https://doi.org/10.1006/abio.2001.5116
Publikováno v:
Cytometry. 37:314-319
Background: The alkaline phosphatase (AP) substrate 2-(58-chloro-28-phosphoryloxyphenyl)-6-chloro-4-(3H)-quinazolinone (ELF t -97 for enzyme-labeled fluorescence) has been found useful for the histochemical detection of endogenous AP activity and AP-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ffdeb24e9a6bc5bfd1f6e114d0baf28b
https://doi.org/10.1002/(sici)1097-0320(19991201)37:4<314::aid-cyto9>3.0.co
https://doi.org/10.1002/(sici)1097-0320(19991201)37:4<314::aid-cyto9>3.0.co
Autor:
Victoria L. Singer, Ching-Ying Cheung, Matthew P. Beaudet, Stephen T. Yue, Laurie J. Jones, Xiaokui Jin, Rabiya S. Tuma
Publikováno v:
Analytical Biochemistry. 268:278-288
The highest sensitivity nucleic acid gel stains developed to date are optimally excited using short-wavelength ultraviolet or visible light. This is a disadvantage for laboratories equipped only with 306- or 312-nm UV transilluminators. We have devel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a1e7845b8b66dd0d1cf0a49ecfd2ff0
https://doi.org/10.1006/abio.1998.3067
https://doi.org/10.1006/abio.1998.3067
Publikováno v:
Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 439:37-47
SYBR Green I nucleic acid gel stain is an unsymmetrical cyanine dye developed for sensitive detection of nucleic acids in electrophoretic gels. Its mechanism of nucleic acid binding is not known, whereas the most commonly used nucleic acid gel stain,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5062dd821954bc8f5e4df017d0c47566
https://doi.org/10.1016/s1383-5718(98)00172-7
https://doi.org/10.1016/s1383-5718(98)00172-7
Publikováno v:
Analytical Biochemistry. 249:228-238
A sensitive assay for detecting double-stranded (ds) DNA in solution is described. This assay employs a new dye, PicoGreen dsDNA quantitation reagent, which becomes intensely fluorescent upon binding nucleic acids. The brightness of this reagent is d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::357824e1f81dfc0079b6d2e1d2144f1c
https://doi.org/10.1006/abio.1997.2177
https://doi.org/10.1006/abio.1997.2177
Publikováno v:
Analytical Biochemistry. 248:168-172
Photography or electronic image acquisition is required to document results obtained from staining protein gels with the fluorescent SYPRO dyes. We found that, when using Polaroid type 667 or 57 instant films, the choice of optical filter combination
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::831a641795e857889102930151057312
https://doi.org/10.1006/abio.1997.2117
https://doi.org/10.1006/abio.1997.2117
Publikováno v:
Cytometry. 27:358-364
We characterized the ability of six SYTO nucleic acid stains and a mitochondrial stain to resolve by flow cytometry camptothecin-induced apoptotic and non-apoptotic cells. Staining live human lymphoid B-cells showed such resolution with SYTO 11, 12,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8c7e5392e7d1a370e30902c6ce7c63b8
https://doi.org/10.1002/(sici)1097-0320(19970401)27:4<358::aid-cyto7>3.0.co
https://doi.org/10.1002/(sici)1097-0320(19970401)27:4<358::aid-cyto7>3.0.co
Autor:
L J Jones, Martin Poot, A G Lugade, J. A. Kramer, Yu-zhong Zhang, K S Wells, D K Hanzel, Rosaria P. Haugland, Victoria L. Singer
Publikováno v:
Journal of Histochemistry & Cytochemistry. 44:1363-1372
Investigation of mitochondrial morphology and function has been hampered because photostable, mitochondrion-specific stains that are retained in fixed, permeabilized cells have not been available. We found that in live cell preparations, the CMXRos a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2697ebf6929d5e2c08ad80fc79e126c9
https://doi.org/10.1177/44.12.8985128
https://doi.org/10.1177/44.12.8985128