A robust system for RNA interference in the chicken using a modified microRNA operon

Autor: Helen Sang, Marysia Placzek, Michael J. McGrew, Gareth R. Howell, Elizabeth M. Manning, Cheryll Tickle, Elizabeth R. Farrell, Melanie A. Sacco, Victoria C. Porteous, Raman M. Das, Nick Van Hateren, Pam A. Halley, Stuart A. Wilson, Venugopal Nair, Fiona Bangs, Kyoji Ohyama, Kate G. Storey

Publikováno v: Das, R M, Van Hateren, N J, Howell, G R, Farrell, E R, Bangs, F K, Porteous, V C, Manning, E M, McGrew, M, Ohyama, K, Sacco, M A, Halley, P A, Sang, H M, Storey, K G, Placzek, M, Tickle, C, Nair, V K & Wilson, S A 2006, ' A robust system for RNA interference in the chicken using a modified microRNA operon ', Developmental Biology, vol. 294, no. 2, pp. 554-63 . https://doi.org/10.1016/j.ydbio.2006.02.020

RNA interference (RNAi) provides an effective method to silence gene expression and investigate gene function. However, RNAi tools for the chicken embryo have largely been adapted from vectors designed for mammalian cells. Here we present plasmid and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d2a9c47579bed48e720321f71619702
https://doi.org/10.1016/j.ydbio.2006.02.020