CD1a promotes systemic manifestations of skin inflammation

Autor: Clare S. Hardman, Yi-Ling Chen, Marcin Wegrecki, Soo Weei Ng, Robert Murren, Davinderpreet Mangat, John-Paul Silva, Rebecca Munro, Win Yan Chan, Victoria O’Dowd, Carl Doyle, Prashant Mori, Andy Popplewell, Jamie Rossjohn, Daniel Lightwood, Graham S. Ogg

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-18 (2022)

Skin inflammation is often accompanied by systemic disease, yet the pathways that regulate this escalation are little known. Here authors show that transgenic expression of human CD1a in mice leads to the escalation of experimental skin inflammation

Externí odkaz: https://doaj.org/article/8f36ea19ce9a4285881dcc232935ca78

Therapeutic Monoclonal Antibodies for Ebola Virus Infection Derived from Vaccinated Humans

Autor: Terry Baker, Francesca R. Donnellan, Geneviève M. Labbé, Andy Popplewell, Alain Townsend, Daniel John Lightwood, Gillian Burgess, Victoria O’Dowd, David McMillan, Jing Jin, Jia Guo, Pramila Rijal, Julie Xiao, Miles W. Carroll, Samara Rosendo Machado, Xiao-Ning Xu, Sean C. Elias, Simon J. Draper, Tommy Rampling, Catherine J. Cherry, Emma Rayner, Ayato Takada, Stuart D. Dowall, Lisa Schimanski, Stephen-Findlay Wilson, Kuan-Ying A. Huang, Emily Barry, Simon C Mendelsohn

Publikováno v: Cell Reports, Vol 27, Iss 1, Pp 172-186.e7 (2019)
186.e7

Summary: We describe therapeutic monoclonal antibodies isolated from human volunteers vaccinated with recombinant adenovirus expressing Ebola virus glycoprotein (EBOV GP) and boosted with modified vaccinia virus Ankara. Among 82 antibodies isolated f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76781ab6af6ee48ad93c397c8c49617f
http://www.sciencedirect.com/science/article/pii/S2211124719303274

Distinctive binding properties of human monoclonal LGI1 autoantibodies determine pathogenic mechanisms

Autor: Victoria O’Dowd, Sofija Paneva, Robert J. Griffin, Jeanne M M Tan, Jorge Ramírez-Franco, Daniel C. Anthony, Sophia Michael, Melanie Ramberger, Diane Marshall, Stéphanie Baulac, Tianrong Yeo, David McMillan, Bo Sun, Daniel John Lightwood, Sarfaraj Topia, Sarosh R. Irani, Oussama El Far, Patrick Waters, Alexander Jeans, Arjune Sen, Jakob Theorell, Neesha Dedi, Rachael Bashford-Rogers, Antonio Berretta, Maria Isabel Leite

Publikováno v: Brain-A Journal of Neurology
Brain-A Journal of Neurology, Oxford University Press (OUP), 2020, 143 (6), pp.1731-1745. ⟨10.1093/brain/awaa104⟩
Brain

See Zekeridou and Pittock (doi:10.1093/brain/awaa153) for a scientific commentary on this article. Ramberger et al. generate patient-derived monoclonal antibodies against LGI1 and describe their epitopes and sequences, plus their pathogenic potential

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14ad8246e839ce359f21afd61ba8bb05
https://pubmed.ncbi.nlm.nih.gov/32543694

The rapid generation of recombinant functional monoclonal antibodies from individual, antigen-specific bone marrow-derived plasma cells isolated using a novel fluorescence-based method

Autor: Daniel John Lightwood, Rebecca J Wootton, Brendon MacKenzie, Welcome Ndlovu, Dominic P White, Alison Clargo, Sophie P. Shaw, Alastair D. G. Lawson, James Snowden, Meryn Griffiths, Michael E. Wright, Joanne E. Compson, Carla R Nowosad, Ashley R Hudson, Victoria O’Dowd, Louise A Cremin, Dale Starkie, Paul Edward Stephens, Laura Ellen Newnham

Publikováno v: mAbs. 6:143-159

Single B cell technologies, which avoid traditional hybridoma fusion and combinatorial display, provide a means to interrogate the naturally-selected antibody repertoire of immunized animals. Many methods enable the sampling of memory B cell subsets,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56308b11fd5cc660cfd935fa796c86df
https://doi.org/10.4161/mabs.27044

The Discovery, Engineering and Characterisation of a Highly Potent Anti-Human IL-13 Fab Fragment Designed for Administration by Inhalation

Autor: Daniel, Lightwood, Victoria, O'Dowd, Bruce, Carrington, Vaclav, Veverka, Mark D, Carr, Markus, Tservistas, Alistair J, Henry, Bryan, Smith, Kerry, Tyson, Sabrina, Lamour, Marguerite, Bracher, Kaushik, Sarkar, Alison, Turner, Alastair D, Lawson, Tim, Bourne, Neil, Gozzard, Roger, Palframan

Publikováno v: Journal of Molecular Biology. 425:577-593

We describe the discovery, engineering and characterisation of a highly potent anti-human interleukin (IL)-13 Fab fragment designed for administration by inhalation. The lead candidate molecule was generated via a novel antibody discovery process, an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e220d3deb7766a555a5341b5e3028cc5
https://doi.org/10.1016/j.jmb.2012.11.036