A Flexible and Original Architecture of Two Unrelated Zinc Fingers Underlies the Role of the Multitask P1 in Rymv Spread

Autor: Vianney Poignavent, François Hoh, Guillaume Terral, Yinshan Yang, François-Xavier Gillet, Jeong-Hyeon Kim, Frédéric Allemand, Eric Lacombe, Christophe Brugidou, Sarah Cianferani, Hélène Déméné, Florence Vignols

Publikováno v: Journal of Molecular Biology
Journal of Molecular Biology, 2022, 434 (16), pp.167715. ⟨10.1016/j.jmb.2022.167715⟩

International audience; Viruses of the sobemovirus genus are plant viruses, most of which generate very important agricultural and financial losses. Among them, the rice yellow mottle virus (RYMV) is one of the most damaging pathogens devastating ric

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::302dcc989a1834d46f8f326adda7b33b
https://doi.org/10.2139/ssrn.4103096

Structural basis of nanobody recognition of grapevine fanleaf virus and of virus resistance loss

Autor: Olivier Lemaire, Christophe Ritzenthaler, Aurélie Marmonier, Sophie Gersch, Bruno P. Klaholz, Léa Ackerer, Corinne Schmitt-Keichinger, Vianney Poignavent, Kamal Hleibieh, Lorène Belval, Serge Muyldermans, Véronique Komar, Caroline Hemmer, Patrick Bron, Ahmed Ghannam, Claude Sauter, Igor Orlov, Pascale Schellenberger, Jean-Michel Hily, Gérard Demangeat, Bernard Lorber, Emmanuelle Vigne

Publikováno v: Proc Natl Acad Sci U S A
Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2020, 117 (20), pp.10848-10855. ⟨10.1073/pnas.1913681117⟩
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2020, 117 (20), pp.10848-10855. ⟨10.1073/pnas.1913681117⟩

International audience; Grapevine fanleaf virus (GFLV) is a picorna-like plant virus transmitted by nematodes that affects vineyards worldwide. Nanobody (Nb)-mediated resistance against GFLV has been created recently, and shown to be highly effective

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65444a63f4cfb5dd46b16e65ea51f14b
https://pubmed.ncbi.nlm.nih.gov/32371486

NMR chemical shift backbone assignment of the viral protein P1 encoded by the African Rice Yellow Mottle Virus

Autor: Vianney Poignavent, Hélène Déméné, Florence Vignols, François-Xavier Gillet, Yinshan Yang

Publikováno v: Biomolecular NMR Assignments
Biomolecular NMR Assignments, Springer, 2019, 13 (2), pp.345-348. ⟨10.1007/s12104-019-09903-z⟩
Biomolecular NMR Assignments, Springer, 2019, 13, pp.345-348. ⟨10.1007/s12104-019-09903-z⟩

International audience; RNA silencing describes a pan-eukaryotic pathway of gene regulation where doubled stranded RNA are processed by the RNAse III enzyme Dicer or homologs. In particular, plants use it as a way to defend themselves against pathoge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01bebe3e1455faf4606abd74a2c0d15d
https://hal.archives-ouvertes.fr/hal-02322880

Construction of High-Quality Camel Immune Antibody Libraries

Autor: Cécile Vincke, Vianney Poignavent, Ema Romão, Christophe Ritzenthaler, Baptiste Monsion, Serge Muyldermans

Publikováno v: Methods in Molecular Biology
Methods in Molecular Biology, 1701, Humana Press, 562 p., 2018, Methods in Molecular Biology, 978-1-4939-7446-7 978-1-4939-7447-4. ⟨10.1007/978-1-4939-7447-4_9⟩
Methods in Molecular Biology ISBN: 9781493974467

Single-domain antibodies libraries of heavy-chain only immunoglobulins from camelids or shark are enriched for high-affinity antigen-specific binders by a short in vivo immunization. Thus, potent binders are readily retrieved from relatively small-si

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e329d86086d663d083959dc4e9a02c2f
https://doi.org/10.1007/978-1-4939-7447-4_9

The RYMV-encoded viral suppressor of RNA silencing P1 is a zinc-binding protein with redox-dependent flexibility

Autor: Christophe Brugidou, Yannick Bessin, Florence Vignols, Alain Van Dorsselaer, Sarah Sanglier-Cianférani, Nathalie Declerck, Diego I. Cattoni, Vianney Poignavent, François Delalande, Jean-Paul Brizard, Stéphanie Petiot-Bécard, François-Xavier Gillet

Publikováno v: Journal of molecular biology. 425(14)

Viral suppressors of RNA interference (VSRs) target host gene silencing pathways, thereby operating important roles in the viral cycle and in host cells, in which they counteract host innate immune responses. However, the molecular mechanisms of VSRs

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5f6cc988f825928839e785eceab7b4a
https://pubmed.ncbi.nlm.nih.gov/23542010