Production monitoring and purification of EBV encoded latent membrane protein 1 expressed and secreted by recombinant baculovirus infected insect cells

Autor: Meij, P, Vervoort, M B, Meijer, C J, Bloemena, E, Middeldorp, J M

Publikováno v: Meij, P, Vervoort, M B, Meijer, C J, Bloemena, E & Middeldorp, J M 2000, ' Production monitoring and purification of EBV encoded latent membrane protein 1 expressed and secreted by recombinant baculovirus infected insect cells ', Journal of Virological Methods, vol. 90, no. 2, pp. 193-204 .
Journal of Virological Methods, 90(2), 193-204. Elsevier

Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) is expressed in malignancies with latency type II and III and is an important transforming protein. To further study this protein LMP1 was expressed by and purified from recombinant ba

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::8477e4a8d68b2cf603fa7fb5319c3752
https://research.vumc.nl/en/publications/c8e62336-37f2-419f-98f4-e614b7f39828

Monitoring of epstein-barr virus DNA load in peripheral blood by quantitative competitive PCR

Autor: Stevens, S J, Vervoort, M B, van den Brule, A J, Meenhorst, P L, Meijer, C J, Middeldorp, J M

Publikováno v: Stevens, S J, Vervoort, M B, van den Brule, A J, Meenhorst, P L, Meijer, C J & Middeldorp, J M 1999, ' Monitoring of epstein-barr virus DNA load in peripheral blood by quantitative competitive PCR ', Journal of Clinical Microbiology, vol. 37, no. 9, pp. 2852-7 .
Journal of Clinical Microbiology, 37(9), 2852-7. American Society for Microbiology

A competitive quantitative PCR (Q-PCR) assay combined with simple silica-based DNA extraction was developed for monitoring of Epstein-Barr virus (EBV) DNA load in unfractionated peripheral blood. The Q-PCR is based on competitive coamplification of a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::00748e222510c7e81e702028c82afab0
https://research.vumc.nl/en/publications/6136c914-6fcf-4a7c-8d44-5f3b1d0316a6

Nucleic acid sequence-based amplification, a new method for analysis of spliced and unspliced Epstein-Barr virus latent transcripts, and its comparison with reverse transcriptase PCR

Autor: Brink, A A, Vervoort, M B, Middeldorp, J M, Meijer, C J, van den Brule, A J

Publikováno v: Brink, A A, Vervoort, M B, Middeldorp, J M, Meijer, C J & van den Brule, A J 1998, ' Nucleic acid sequence-based amplification, a new method for analysis of spliced and unspliced Epstein-Barr virus latent transcripts, and its comparison with reverse transcriptase PCR ', Journal of Clinical Microbiology, vol. 36, no. 11, pp. 3164-9 .
Journal of Clinical Microbiology, 36(11), 3164-9. American Society for Microbiology

Nucleic acid sequence-based amplification (NASBA) assays were developed for direct detection of Epstein-Barr virus (EBV) transcripts encoding EBV nuclear antigen 1 (EBNA1), latent membrane proteins (LMP) 1 and 2, and BamHIA rightward frame 1 (BARF1)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::5b687f4839eba379daa46e2c714fda94
https://research.vumc.nl/en/publications/0482aa69-4404-42ef-8804-ea2128af38d0

Functional CD4(+) and CD8(+) T-cell responses induced by autologous mitomycin C treated Epstein-Barr virus transformed lymphoblastoid cell lines.

Autor: Meij P; Department of Pathology, University Hospital Vrije Universiteit, 1007 MB Amsterdam, The Netherlands., Bloemena E, Palmen N, Brink A, Vervoort MB, Meijer CJ, Middeldorp JM

Publikováno v: Cellular immunology [Cell Immunol] 2001 Feb 25; Vol. 208 (1), pp. 25-33.

Bioreactor-scale production and one-step purification of Epstein-Barr nuclear antigen 1 expressed in baculovirus-infected insect cells.

Autor: Meij P; Department of Pathology, Academic Hospital Vrije Universiteit, Amsterdam, The Netherlands. p,meij@azvu.nl, Vervoort MB, de Gooijer K, Bloemena E, Meijer CJ, Middeldorp JM

Publikováno v: Protein expression and purification [Protein Expr Purif] 2000 Nov; Vol. 20 (2), pp. 324-33.