Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Veronika E. Anisimova"'
Autor:
Alex S. Shcheglov, Veronika E. Anisimova, Ekaterina A. Bogdanova, Alexey N. Nekrasov, Dmitry A. Shagin, Denis V. Rebrikov, Ekaterina V. Barsova, Sergey Lukyanov
Publikováno v:
Gene. 418:41-48
Kamchatka crab duplex-specific nuclease (Par_DSN) has been classified as a member of the family of DNA/RNA non-specific beta-beta-alpha metal finger (bba-Me-finger) nucleases, the archetype of which is the nuclease from Serratia marcescens. Although
Autor:
A. S. Shcheglov, Sergey Lukyanov, Pavel A. Zhulidov, Denis V. Rebrikov, Veronika E. Anisimova, Dmitry B. Staroverov
Publikováno v:
Biochemistry (Moscow). 71:513-519
We overexpressed duplex-specific nuclease (DSN) from Kamchatka crab in Escherichia coli cells and developed procedures for purification, renaturation, and activation of this protein. We demonstrated identity of the properties of the native and recomb
Autor:
Veronika E. Anisimova, Alex S. Shcheglov, Ekaterina A. Bogdanova, Sergey Lukyanov, Ekaterina V. Barsova
Publikováno v:
Biotechnology letters. 31(2)
Using random mutagenesis of the gene encoding duplex-specific nuclease from the king crab we found a new mutant that retained all properties of the wild-type protein, but exhibited a much lower thermal stability. This enzyme, denoted thermolabile dup
Autor:
Sergey Lukyanov, Dmitry A. Shagin, Dmitry B. Staroverov, Valery A. Rasskazov, Valery B. Kozhemyako, Vagner Ll, Veronika E. Anisimova, Rustam Ziganshin, Natalia I Menzorova, Alex S. Shcheglov, Denis V. Rebrikov
Publikováno v:
BMC Biochemistry, Vol 9, Iss 1, p 14 (2008)
BMC Biochemistry
BMC Biochemistry
Background Nucleases, which are key components of biologically diverse processes such as DNA replication, repair and recombination, antiviral defense, apoptosis and digestion, have revolutionized the field of molecular biology. Indeed many standard m