A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations.

Autor: Anderson MAE; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.; The Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK., Gonzalez E; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.; Animal and Plant Health Agency, Woodham Lane, Addlestone, Surrey, KT15 3NB, UK., Edgington MP; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.; The Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK., Ang JXD; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.; The Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK., Purusothaman DK; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.; MRC-University of Glasgow Centre for Virus Research, Henry Wellcome Building, 464 Bearsden Road, Glasgow, G61 1QH, UK., Shackleford L; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.; The Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK., Nevard K; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK., Verkuijl SAN; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.; Department of Biology, University of Oxford, 11a Mansfield Road, Oxford, OX1 3SZ, UK., Harvey-Samuel T; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK., Leftwich PT; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, Norfolk, NR4 7TJ, UK., Esvelt K; Media Laboratory, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA., Alphey L; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK. luke.alphey@york.ac.uk.; The Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK. luke.alphey@york.ac.uk.

Publikováno v: Nature communications [Nat Commun] 2024 Jan 25; Vol. 15 (1), pp. 729. Date of Electronic Publication: 2024 Jan 25.

A CRISPR endonuclease gene drive reveals distinct mechanisms of inheritance bias.

Autor: Verkuijl SAN; Mathematical Ecology Research Group, Department of Biology, University of Oxford, 11a Mansfield Road, Oxford, OX1 3SZ, UK.; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK., Gonzalez E; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK.; The Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK., Li M; School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA, 92093, USA., Ang JXD; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK.; The Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK., Kandul NP; School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA, 92093, USA., Anderson MAE; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK.; The Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK., Akbari OS; School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA, 92093, USA., Bonsall MB; Mathematical Ecology Research Group, Department of Biology, University of Oxford, 11a Mansfield Road, Oxford, OX1 3SZ, UK., Alphey L; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK. luke.alphey@york.ac.uk.; The Department of Biology, University of York, Wentworth Way, York, YO10 5DD, UK. luke.alphey@york.ac.uk.

Publikováno v: Nature communications [Nat Commun] 2022 Nov 21; Vol. 13 (1), pp. 7145. Date of Electronic Publication: 2022 Nov 21.

The Challenges in Developing Efficient and Robust Synthetic Homing Endonuclease Gene Drives.

Autor: Verkuijl SAN; Arthropod Genetics, The Pirbright Institute, Pirbright, United Kingdom.; Department of Zoology, University of Oxford, Oxford, United Kingdom., Ang JXD; Arthropod Genetics, The Pirbright Institute, Pirbright, United Kingdom., Alphey L; Arthropod Genetics, The Pirbright Institute, Pirbright, United Kingdom., Bonsall MB; Department of Zoology, University of Oxford, Oxford, United Kingdom., Anderson MAE; Arthropod Genetics, The Pirbright Institute, Pirbright, United Kingdom.

Publikováno v: Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2022 Mar 28; Vol. 10, pp. 856981. Date of Electronic Publication: 2022 Mar 28 (Print Publication: 2022).

Cas13b-dependent and Cas13b-independent RNA knockdown of viral sequences in mosquito cells following guide RNA expression.

Autor: Tng PYL; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK.; Pathobiology and Population Sciences, The Royal Veterinary College, Hawkshead Lane, Hertfordshire, AL9 7TA, UK., Carabajal Paladino L; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK., Verkuijl SAN; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK.; Department of Zoology, University of Oxford, 11a Mansfield Road, Oxford, OX1 3SZ, UK., Purcell J; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK.; Vector Biology Department, Liverpool School of Tropical Medicine, Liverpool, L3 5QA, UK., Merits A; Institute of Technology, University of Tartu, Nooruse 1, Tartu, 50411, Estonia., Leftwich PT; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK.; School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK., Fragkoudis R; Arbovirus Pathogenesis, The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK.; The University of Nottingham, School of Veterinary Medicine and Science, Sutton Bonington, Loughborough, LE12 5RD, UK., Noad R; Pathobiology and Population Sciences, The Royal Veterinary College, Hawkshead Lane, Hertfordshire, AL9 7TA, UK., Alphey L; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0NF, UK. luke.alphey@pirbright.ac.uk.

Publikováno v: Communications biology [Commun Biol] 2020 Jul 31; Vol. 3 (1), pp. 413. Date of Electronic Publication: 2020 Jul 31.