Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Vera Pingoud"'
Autor:
Alfred Pingoud, Vera Pingoud, Wolfgang Wende, Jürgen Alves, Monika Reuter, Letif Mones, Monika Fuxreiter, Albert Jeltsch, Peter Friedhoff
Publikováno v:
Journal of Molecular Biology. 393:140-160
Restriction endonucleases of the PD...D/EXK family need Mg(2+) for DNA cleavage. Whereas Mg(2+) (or Mn(2+)) promotes catalysis, Ca(2+) (without Mg(2+)) only supports DNA binding. The role of Mg(2+) in DNA cleavage by restriction endonucleases has eli
Publikováno v:
Nucleic Acids Research
Hydrolytic deamination of cytosines in DNA creates uracil and, if unrepaired, these lesions result in C to T mutations. We have suggested previously that a possible way in which cells may prevent or reduce this chemical reaction is through the bindin
Autor:
Kristin Eisenschmidt, Vera Pingoud, Thomas Lanio, Albert Jeltsch, András Simoncsits, Alfred Pingoud, Wolfgang Wende
Publikováno v:
Nucleic Acids Research
Specific cleavage of large DNA molecules at few sites, necessary for the analysis of genomic DNA or for targeting individual genes in complex genomes, requires endonucleases of extremely high specificity. Restriction endonucleases (REase) that recogn
Autor:
Elena A. Kubareva, Tatiana S. Oretskaya, Anna Sudina, A. Pingoud, Timofei S. Zatsepin, Vera Pingoud
Publikováno v:
Biochemistry (Moscow). 70:941-947
Properties of 2'-aldehyde-containing double stranded DNAs (dsDNAs) have been studied for the first time as substrate analogs of the restriction endonuclease SsoII. These reactive oligonucleotides were successfully cross-linked to the restriction endo
Autor:
Hildegard Geyer, Anna Sudina, Elena A. Kubareva, Gerhild Lüder, Vera Pingoud, Rudi Lurz, Alfred Pingoud, Janusz M. Bujnicki, Richard D. Morgan
Publikováno v:
Journal of Biological Chemistry. 280:4289-4298
How restriction enzymes with their different specificities and mode of cleavage evolved has been a long standing question in evolutionary biology. We have recently shown that several Type II restriction endonucleases, namely SsoII (downward arrow CCN
Autor:
Vera Pingoud, Alfred Pingoud, Elena A. Kubareva, Hildegard Geyer, Janusz M. Bujnicki, Rudolf Geyer
Publikováno v:
Mol. BioSyst.. 1:135-141
Specific protein-nucleic acid interactions are of paramount importance for the propagation, maintenance and expression of genetic information. Restriction endonucleases serve as model systems to study the mechanisms of DNA recognition by proteins. Ss
Publikováno v:
ChemBioChem. 5:206-213
Homing endonucleases are extremely specific endodeoxyribonucleases. In vivo, these enzymes confer mobility on their genes by inducing a very specific double-strand cut in cognate alleles that lack the cooling sequence for the homing endonuclease; the
Autor:
Gerhild Lüder, Alfred Pingoud, Elena A. Kubareva, Charlotte Conzelmann, Steffen Kinzebach, Anna Sudina, Shuang-Yong Xu, Vera Pingoud, Rudi Lurz, Janusz M. Bujnicki, Valerie Metelev
Publikováno v:
Journal of Molecular Biology. 329:913-929
We present here the first detailed biochemical analysis of an archaeal restriction enzyme. PspGI shows sequence similarity to SsoII, EcoRII, NgoMIV and Cfr10I, which recognize related DNA sequences. We demonstrate here that PspGI, like SsoII and unli
Publikováno v:
Journal of Biological Chemistry. 277:14288-14293
The EcoRII homodimer engages two of its recognition sequences (5'-CCWGG) simultaneously and is therefore a type IIE restriction endonuclease. To identify the amino acids of EcoRII that interact specifically with the recognition sequence, we photocros
Autor:
Tatiana S. Oretskaya, Anna S. Karyagina, Alfred Pingoud, Hubert Thole, Elena A. Kubareva, Vera Pingoud
Publikováno v:
Nucleic Acids Research. 28:1085-1091
A target sequence-specific DNA binding region of the restriction endonuclease Sso II was identified by photocross-linking with an oligodeoxynucleotide duplex which was substituted with 5-iododeoxy-uridine (5-IdU) at the central position of the Sso II