Live-cell micromanipulation of a genomic locus reveals interphase chromatin mechanics.

Autor: Keizer, Veer I. P., Grosse-Holz, Simon, Woringer, Maxime, Zambon, Laura, Aizel, Koceila, Bongaerts, Maud, Delille, Fanny, Kolar-Znika, Lorena, Scolari, Vittore F., Hoffmann, Sebastian, Banigan, Edward J., Mirny, Leonid A., Dahan, Maxime, Fachinetti, Daniele daniele.fachinetti@curie.fr, Coulon, Antoine antoine.coulon@curie.fr

Publikováno v: Science. 7/29/2022, Vol. 377 Issue 6605, p489-495. 7p. 3 Color Photographs.

Live-cell micromanipulation of a genomic locus reveals interphase chromatin mechanics

Autor: Veer I. P. Keizer, Simon Grosse-Holz, Maxime Woringer, Laura Zambon, Koceila Aizel, Maud Bongaerts, Fanny Delille, Lorena Kolar-Znika, Vittore F. Scolari, Sebastian Hoffmann, Edward J. Banigan, Leonid A. Mirny, Maxime Dahan, Daniele Fachinetti, Antoine Coulon

Publikováno v: Science
Science, American Association for the Advancement of Science (AAAS), 2022, 377 (6605), pp.489-495. ⟨10.1126/science.abi9810⟩
Science, 2022, 377 (6605), pp.489-495. ⟨10.1126/science.abi9810⟩

International audience; Our understanding of the physical principles organizing the genome in the nucleus is limited by the lack of tools to directly exert and measure forces on interphase chromosomes in vivo and probe their material nature. Here, we

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5a8c5c01c224dc742c41e42815ac60c
https://pubmed.ncbi.nlm.nih.gov/35901166

Quantitation of glucocorticoid receptor DNA-binding dynamics by single-molecule microscopy and FRAP.

Autor: Femke L Groeneweg, Martin E van Royen, Susanne Fenz, Veer I P Keizer, Bart Geverts, Jurrien Prins, E Ron de Kloet, Adriaan B Houtsmuller, Thomas S Schmidt, Marcel J M Schaaf

Publikováno v: PLoS ONE, Vol 9, Iss 3, p e90532 (2014)

Recent advances in live cell imaging have provided a wealth of data on the dynamics of transcription factors. However, a consistent quantitative description of these dynamics, explaining how transcription factors find their target sequences in the va

Externí odkaz: https://doaj.org/article/d47c3fc061a64d269c690076030ab728

Repetitive switching between DNA-binding modes enables target finding by the glucocorticoid receptor

Autor: Bart Geverts, Stefano Coppola, Adriaan B. Houtsmuller, Thomas Schmidt, Veer I. P. Keizer, Martin E. van Royen, Marcel J. M. Schaaf

Publikováno v: Journal of Cell Science, 132(5):jcs217455. Company of Biologists Ltd
Journal of Cell Science, 132(5), jcs217445. COMPANY BIOLOGISTS LTD

Transcription factor mobility is a determining factor in the regulation of gene expression. Here, we have studied the intranuclear dynamics of the glucocorticoid receptor (GR) by using fluorescence recovery after photobleaching and single-molecule mi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50e3cca998faabe84cac66b0d3b6200e
https://pure.eur.nl/en/publications/7312854e-1057-4523-95b0-5be64cbd1df0

Depth-of-Focus Correction in Single-Molecule Data Allows Analysis of 3D Diffusion of the Glucocorticoid Receptor in the Nucleus.

Autor: Harkes, Rolf¹, Keizer, Veer I. P.², Schaaf, Marcel J. M.², Schmidt, Thomas¹ schmidt@physics.leidenuniv.nl

Publikováno v: PLoS ONE. 10/11/2015, Vol. 10 Issue 10, p1-13. 13p.