Landscape analysis of escape variants identifies SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization

Autor: Michael S. Diamond, Errico Jm, Ali H. Ellebedy, Haiyan Zhao, Louis Marie Bloyet, Buchser Wj, Whelan Spj, Alford B, Elitza S. Theel, VanBlargan La, Liebeskind Mj, Paul W. Rothlauf, Rita E. Chen, Fremont Dh, Zhuoming Liu, Stumpf S

Publikováno v: bioRxiv

Although neutralizing antibodies against the SARS-CoV-2 spike (S) protein are a goal of COVID-19 vaccines and have received emergency use authorization as therapeutics, viral escape mutants could compromise their efficacy. To define the immune-select

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36c0e300c55c879937f19b168897d2f1
https://doi.org/10.1101/2020.11.06.372037

Genetic loci regulate Sarbecovirus pathogenesis: A comparison across mice and humans.

Autor: Schäfer A; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Electronic address: aschaefe@email.unc.edu., Gralinski LE; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Electronic address: lgralins@email.unc.edu., Leist SR; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Hampton BK; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Mooney MA; Knight Cancer Institute, Oregon Health & Science University, Portland, OR, USA; Division of Bioinformatics and Computational Biology, Oregon Health & Science University, Portland, OR, USA; Department of Medical Informatics and Clinical Epidemiology, Oregon Health & Science University, Portland, OR, USA., Jensen KL; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Graham RL; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Agnihothram S; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Jeng S; Knight Cancer Institute, Oregon Health & Science University, Portland, OR, USA; Oregon Clinical and Translational Research Institute, Oregon Health & Science University, Portland, OR, USA., Chamberlin S; Division of Bioinformatics and Computational Biology, Oregon Health & Science University, Portland, OR, USA; Department of Medical Informatics and Clinical Epidemiology, Oregon Health & Science University, Portland, OR, USA., Bell TA; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Scobey DT; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Linnertz CL; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., VanBlargan LA; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA., Thackray LB; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA., Hock P; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Miller DR; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Shaw GD; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Diamond MS; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA; Department of Pathology & Immunology2, Washington University School of Medicine, St. Louis, MO, USA; Department of Molecular Microbiology3, Washington University School of Medicine, St. Louis, MO, USA., de Villena FPM; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., McWeeney SK; Knight Cancer Institute, Oregon Health & Science University, Portland, OR, USA; Division of Bioinformatics and Computational Biology, Oregon Health & Science University, Portland, OR, USA; Department of Medical Informatics and Clinical Epidemiology, Oregon Health & Science University, Portland, OR, USA; Oregon Clinical and Translational Research Institute, Oregon Health & Science University, Portland, OR, USA., Heise MT; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Rapidly Emerging Antiviral Drug Discovery Initiative, University of North Carolina, Chapel Hill NC, USA., Menachery VD; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA; Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston TX, USA; Department of Pathology and Center for Biodefense & Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX, USA., Ferris MT; Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Electronic address: mtferris@email.unc.edu., Baric RS; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Rapidly Emerging Antiviral Drug Discovery Initiative, University of North Carolina, Chapel Hill NC, USA. Electronic address: rsbaric@email.unc.edu.

Publikováno v: Virus research [Virus Res] 2024 Jun; Vol. 344, pp. 199357. Date of Electronic Publication: 2024 Mar 23.

A broadly reactive antibody targeting the N-terminal domain of SARS-CoV-2 spike confers Fc-mediated protection.

Autor: Adams LJ; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA., VanBlargan LA; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA., Liu Z; Department of Molecular Microbiology, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA., Gilchuk P; Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA., Zhao H; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA., Chen RE; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA., Raju S; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA., Chong Z; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA., Whitener BM; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA., Shrihari S; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA., Jethva PN; Department of Chemistry, Washington University, St. Louis, MO 63130, USA., Gross ML; Department of Chemistry, Washington University, St. Louis, MO 63130, USA., Crowe JE Jr; Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA., Whelan SPJ; Department of Molecular Microbiology, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA., Diamond MS; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA; Department of Molecular Microbiology, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA; Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO, USA. Electronic address: mdiamond@wustl.edu., Fremont DH; Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Molecular Microbiology, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA; Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO, USA. Electronic address: fremont@wustl.edu.

Publikováno v: Cell reports. Medicine [Cell Rep Med] 2023 Dec 19; Vol. 4 (12), pp. 101305. Date of Electronic Publication: 2023 Nov 30.

prM-reactive antibodies reveal a role for partially mature virions in dengue virus pathogenesis.

Autor: Dowd KA; Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, Bethesda, MD 20892., Sirohi D; Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.; Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN 47907., Speer SD; Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, Bethesda, MD 20892., VanBlargan LA; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110., Chen RE; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110., Mukherjee S; Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, Bethesda, MD 20892., Whitener BM; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110., Govero J; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110., Aleshnick M; Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, Bethesda, MD 20892., Larman B; Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, Bethesda, MD 20892., Sukupolvi-Petty S; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110., Sevvana M; Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.; Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN 47907., Miller AS; Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.; Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN 47907., Klose T; Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.; Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN 47907., Zheng A; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461., Koenig S; MacroGenics, Inc., Rockville, MD 20850., Kielian M; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461., Kuhn RJ; Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.; Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN 47907., Diamond MS; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110.; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110., Pierson TC; Viral Pathogenesis Section, Laboratory of Viral Diseases, National Institutes of Health, Bethesda, MD 20892.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Jan 17; Vol. 120 (3), pp. e2218899120. Date of Electronic Publication: 2023 Jan 13.

Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants.

Autor: Zhang JZ; Department of Biochemistry, University of Washington, Seattle, WA, USA. jason.zhang0428@gmail.com.; Institute for Protein Design, University of Washington, Seattle, WA, USA. jason.zhang0428@gmail.com., Yeh HW; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Institute for Protein Design, University of Washington, Seattle, WA, USA., Walls AC; Department of Biochemistry, University of Washington, Seattle, WA, USA., Wicky BIM; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Institute for Protein Design, University of Washington, Seattle, WA, USA., Sprouse KR; Department of Biochemistry, University of Washington, Seattle, WA, USA., VanBlargan LA; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA., Treger R; Department of Laboratory Medicine & Pathology, University of Washington, Seattle, WA, USA., Quijano-Rubio A; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Bioengineering, University of Washington, Seattle, WA, USA., Pham MN; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Institute for Protein Design, University of Washington, Seattle, WA, USA., Kraft JC; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Institute for Protein Design, University of Washington, Seattle, WA, USA., Haydon IC; Institute for Protein Design, University of Washington, Seattle, WA, USA., Yang W; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Institute for Protein Design, University of Washington, Seattle, WA, USA., DeWitt M; Institute for Protein Design, University of Washington, Seattle, WA, USA., Bowen JE; Department of Biochemistry, University of Washington, Seattle, WA, USA., Chow CM; Institute for Protein Design, University of Washington, Seattle, WA, USA., Carter L; Institute for Protein Design, University of Washington, Seattle, WA, USA., Ravichandran R; Institute for Protein Design, University of Washington, Seattle, WA, USA., Wener MH; Department of Laboratory Medicine & Pathology, University of Washington, Seattle, WA, USA., Stewart L; Institute for Protein Design, University of Washington, Seattle, WA, USA., Veesler D; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA., Diamond MS; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, USA.; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA.; Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, Saint Louis, MO, USA., Greninger AL; Department of Laboratory Medicine & Pathology, University of Washington, Seattle, WA, USA.; Vaccine and Infectious Diseases Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA., Koelle DM; Department of Laboratory Medicine & Pathology, University of Washington, Seattle, WA, USA.; Vaccine and Infectious Diseases Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.; Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA, USA.; Translational Research Program, Benaroya Research Institute at Virginia Mason, Seattle, WA, USA.; Department of Global Health, University of Washington, Seattle, WA, USA., Baker D; Department of Biochemistry, University of Washington, Seattle, WA, USA. dabaker@uw.edu.; Institute for Protein Design, University of Washington, Seattle, WA, USA. dabaker@uw.edu.; Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA. dabaker@uw.edu.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2022 Sep; Vol. 40 (9), pp. 1336-1340. Date of Electronic Publication: 2022 Apr 28.