Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Valmik K. Vyas"'
Autor:
Valmik K. Vyas, Douglas A. Bernstein
Publikováno v:
Journal of Microbiology & Biology Education, Vol 20, Iss 3 (2019)
Central dogma, transformation, and genome editing are key biological concepts for which junior scientists must gain experience during training. Here we present an exercise that introduces these concepts in a single practical laboratory exercise. Our
Externí odkaz:
https://doaj.org/article/49608ca699394ec9a28a316c0e36aa64
Autor:
Valmik K. Vyas, G. Guy Bushkin, Douglas A. Bernstein, Matthew A. Getz, Magdalena Sewastianik, M. Inmaculada Barrasa, David P. Bartel, Gerald R. Fink
Publikováno v:
mSphere, Vol 3, Iss 2 (2018)
ABSTRACT We have created new vectors for clustered regularly interspaced short palindromic repeat (CRISPR) mutagenesis in Candida albicans, Saccharomyces cerevisiae, Candida glabrata, and Naumovozyma castellii. These new vectors permit a comparison o
Externí odkaz:
https://doaj.org/article/fc3e689ff07143b294c2db074a0e1a1e
Autor:
Karin Strijbis, Fikadu G Tafesse, Gregory D Fairn, Martin D Witte, Stephanie K Dougan, Nicki Watson, Eric Spooner, Alexandre Esteban, Valmik K Vyas, Gerald R Fink, Sergio Grinstein, Hidde L Ploegh
Publikováno v:
PLoS Pathogens, Vol 9, Iss 6, p e1003446 (2013)
Phagocytosis of the opportunistic fungal pathogen Candida albicans by cells of the innate immune system is vital to prevent infection. Dectin-1 is the major phagocytic receptor involved in anti-fungal immunity. We identify two new interacting protein
Externí odkaz:
https://doaj.org/article/2cd6c464b61041a582a289786a0820d6
Autor:
Marina, Zoppo, Mariagrazia Di, Luca, Santiago N, Villarreal, Noemi, Poma, M Inmaculada, Barrasa, Daria, Bottai, Valmik K, Vyas, Arianna, Tavanti
Publikováno v:
Future microbiology. 14
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8183c9a9140e6a29fb904f10b8d6100e
https://pubmed.ncbi.nlm.nih.gov/31659913
https://pubmed.ncbi.nlm.nih.gov/31659913
Autor:
Stijn Mertens, Brigida Gallone, Jan Steensels, Beatriz Herrera-Malaver, Jeroen Cortebeek, Robbe Nolmans, Veerle Saels, Valmik K. Vyas, Kevin J. Verstrepen
Publikováno v:
PLoS ONE, Vol 14, Iss 10, p e0224525 (2019)
[This corrects the article DOI: 10.1371/journal.pone.0209124.].
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96ae47ad73b251eb8b3e0d25a988ed20
https://doaj.org/article/0ff1c23e0c9f4f309186fcbec6d4156e
https://doaj.org/article/0ff1c23e0c9f4f309186fcbec6d4156e
Autor:
Douglas A. Bernstein, Valmik K. Vyas
Publikováno v:
Journal of Microbiology & Biology Education, Vol 20, Iss 3 (2019)
Journal of Microbiology & Biology Education
Journal of Microbiology & Biology Education
Central dogma, transformation, and genome editing are key biological concepts for which junior scientists must gain experience during training. Here we present an exercise that introduces these concepts in a single practical laboratory exercise. Our
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f02c6b9ccb1ade802e49f0307e36e4b4
https://journals.asm.org/doi/10.1128/jmbe.v20i3.1809
https://journals.asm.org/doi/10.1128/jmbe.v20i3.1809
Autor:
Arianna Tavanti, Daria Bottai, Valmik K. Vyas, Mariagrazia Di Luca, Santiago N Villarreal, M. Inmaculada Barrasa, Noemi Poma, Marina Zoppo
Aim: In this study, the CRISPR gene-editing approach was used to simultaneously inactivate all three members of the ALS gene family in the opportunistic pathogen Candida orthopsilosis. Materials & methods: Using a single gRNA and repair template, CRI
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56c7e9fa24f574ed8096bb983866a931
https://hdl.handle.net/11568/1019022
https://hdl.handle.net/11568/1019022
Autor:
Flavia De Bernardis, Cosmeri Rizzato, Antonella Lupetti, Arianna Tavanti, Valmik K. Vyas, Annarita Stringaro, Daria Bottai, Christoph Schaudinn, Mauro Franco, Mariagrazia Di Luca, M. Inmaculada Barrasa, Marina Zoppo
Publikováno v:
Microbiological Research. 231:126351
The ability of yeast to adhere to biotic and abiotic surfaces represents an essential trait during the early stages of infection. Agglutinin-like sequence (Als) cell-wall proteins play a key role in adhesion of Candida species. Candida parapsilosis g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f64009bf34b3ccc60c7ecfa9ad6b0512
https://doi.org/10.1016/j.micres.2019.126351
https://doi.org/10.1016/j.micres.2019.126351
Publikováno v:
Journal of Visualized Experiments.
This method describes the efficient CRISPR mediated genome editing of the diploid human fungal pathogen Candida albicans. CRISPR-mediated genome editing in C. albicans requires Cas9, guide RNA, and repair template. A plasmid expressing a yeast codon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5eaa690d64dd21303f4d07d81aa7e235
https://doi.org/10.3791/58764-v
https://doi.org/10.3791/58764-v
Autor:
Brigida Gallone, Jan Steensels, Kevin J. Verstrepen, Robbe Nolmans, Veerle Saels, Jeroen Cortebeek, Beatriz Herrera-Malaver, Stijn Mertens, Valmik K. Vyas
Publikováno v:
PLoS ONE
PLoS ONE, Vol 14, Iss 1, p e0209124 (2019)
PLoS ONE, Vol 14, Iss 1, p e0209124 (2019)
Today's beer market is challenged by a decreasing consumption of traditional beer styles and an increasing consumption of specialty beers. In particular, lager-type beers (pilsner), characterized by their refreshing and unique aroma and taste, yet ve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52befcd7fc8957da9fdf814dbd91ff2d
https://pubmed.ncbi.nlm.nih.gov/31648239
https://pubmed.ncbi.nlm.nih.gov/31648239