Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Valerie, Burland"'
Autor:
Valerie Burland, Noriko Kusukawa
Publikováno v:
BioTechniques, Vol 23, Iss 6, Pp 1070-1075 (1997)
We demonstrate a rapid cloning and sequencing strategy for kilobase-size DNA segments using DNase I and long PCR. In a single-tube protocol, deletions were formed in a plasmid insert by two enzymatic cuts, one at a fixed site and one at random. The d
Externí odkaz:
https://doaj.org/article/55065fa9f01f4b55a42025fcdf745f33
Publikováno v:
BioTechniques, Vol 21, Iss 1, Pp 142-144 (1996)
Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel streng
Externí odkaz:
https://doaj.org/article/1da290c884c54d5fa202c6f5102a9946
Publikováno v:
Journal of Biotechnology. 160:72-79
The last few years have witnessed rapid progress in bacterial genome engineering. The long-established, standard ways of DNA synthesis, modification, transfer into living cells, and incorporation into genomes have given way to more effective, large-s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2db268a46364d02e43026e7b914d5281
https://doi.org/10.1016/j.jbiotec.2012.02.012
https://doi.org/10.1016/j.jbiotec.2012.02.012
Autor:
Anna I. Rissman, Jon Whitmore, Panna Shetty, Bryan S. Biehl, Michael Rusch, Valerie Burland, Yu Qiu, Bob Mau, Jeremy D. Glasner, Eric Neeno-Eckwall, Sara L. Worzella, Lorie Shaull, Sam Zaremba, Silpa Thotakura, Guy Plunkett, Frederick R. Blattner, Nicole T. Perna, Aaron E. Darling, Paul Liss, Thomas Hampton, John M. Greene, Eric L. Cabot, David J. Baumler, David Pot, Bradley D. Anderson, Matthew Shaker, Joel Fedorko
Publikováno v:
Nucleic Acids Research
ERIC, the Enteropathogen Resource Integration Center (www.ericbrc.org), is a new web portal serving as a rich source of information about enterobacteria on the NIAID established list of Select Agents related to biodefense - diarrheagenic Escherichia
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46c207c1521a7be3124687479cc7967a
https://doi.org/10.1093/nar/gkm973
https://doi.org/10.1093/nar/gkm973
Nucleotide Sequence Analysis of the Enteropathogenic Escherichia coli Adherence Factor Plasmid pMAR7
Autor:
Adam T. Boutin, Rogéria Keller, Valerie Burland, Debra J. Rose, Carl Brinkley, Frederick R. Blattner, Sara A. Klink, James B. Kaper
Publikováno v:
Infection and Immunity. 74:5408-5413
The complete nucleotide sequence was determined for pMAR7, an enteropathogenic Escherichia coli (EPEC) adherence factor (EAF) plasmid that contains genes encoding a type IV attachment pilus (Bfp) and the global virulence regulator per . Prototypic EA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::546f23882fa3784acfaff7b466379631
https://doi.org/10.1128/iai.01840-05
https://doi.org/10.1128/iai.01840-05
Autor:
Gregory P. Fournier, L. J. Runyen-Janecky, Guy Plunkett, W. Deng, Shiguo Zhou, Valerie Burland, David C. Schwartz, Aaron E. Darling, George F. Mayhew, Nicole T. Perna, Frederick R. Blattner, Malabi M. Venkatesan, J. Wei, Debra J. Rose, Marcia B. Goldberg, Shelley M. Payne, Bob Mau
Publikováno v:
Infection and Immunity. 71:2775-2786
We determined the complete genome sequence ofShigella flexneriserotype 2a strain 2457T (4,599,354 bp).Shigellaspecies cause >1 million deaths per year from dysentery and diarrhea and have a lifestyle that is markedly different from those of closely r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0cb0291f45fc21702d1a65443a4b8d4d
https://doi.org/10.1128/iai.71.5.2775-2786.2003
https://doi.org/10.1128/iai.71.5.2775-2786.2003
Autor:
Nicole T. Perna, Frederick R. Blattner, Fabiola Avelino-Flores, Alfredo G. Torres, James B. Kaper, Jorge A. Girón, Valerie Burland
Publikováno v:
Infection and Immunity. 70:5416-5427
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is recognized as a significant enteric pathogen that has been implicated in numerous outbreaks worldwide (reviewed in reference 24). This organism colonizes the intestine and can cause bloody or nonbl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::722fc6256666aaa3c8e47360cd2accc7
https://doi.org/10.1128/iai.70.10.5416-5427.2002
https://doi.org/10.1128/iai.70.10.5416-5427.2002
Autor:
Malabi M. Venkatesan, Valerie Burland
This chapter discusses the genome-scale analysis of virulence plasmids: the contribution of plasmid-borne virulence genes to enterobacterial pathogenesis. The spectrum of diseases caused by Escherichia coli and other enteric bacteria is due to the ac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2e61cf3e635e029bf49d75d0496c0755
https://doi.org/10.1128/9781555817732.ch18
https://doi.org/10.1128/9781555817732.ch18
Autor:
David C. Schwartz, Arvind Ramanathan, Alex Lim, John Skiadas, Rong Qi, Frederick R. Blattner, Bhubaneswar Mishra, Jennifer Apodoca, Galex Yen, Jeremiah D. Hackett, Konstantinos Potamousis, Jieyi Lin, Chunhong Tao, Valerie Burland, Bob Mau, Guy Plunkett, Nicole T. Perna, Thomas Anantharaman, Eileen T. Dimalanta
Publikováno v:
Genome Research. 11:1584-1593
We have constructed NheI and XhoI optical maps ofEscherichia coli O157:H7 solely from genomic DNA molecules to provide a uniquely valuable scaffold for contig closure and sequence validation. E. coli O157:H7 is a common pathogen found in contaminated
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::181e318353feb4c0fb4449cb32ea4a31
https://doi.org/10.1101/gr.172101
https://doi.org/10.1101/gr.172101
Autor:
Jacquelyn M. O'Connor, Noriko Kusukawa, Valerie Burland, Wendy R. Baubonis, W. Edward Highsmith, Mark M. Garner, Foner P. Curtis, Arun J. Nataraj, Qian Jin
Publikováno v:
Electrophoresis. 20:1186-1194
A systematic characterization of the effects of important physical parameters on the sensitivity and specificity of methods in searching for unknown base changes (mutations or single nucleotide polymorphisms) over a relatively long DNA segment has no
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::547efdacc6e5d5960432aa8509b4e82b
https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.0.co
https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.0.co