Výsledky vyhledávání - "Valeria A. Risso"

Akademický článek

Hinge-shift mechanism as a protein design principle for the evolution of β-lactamases from substrate promiscuity to specificity

Autor: Tushar Modi, Valeria A. Risso, Sergio Martinez-Rodriguez, Jose A. Gavira, Mubark D. Mebrat, Wade D. Van Horn, Jose M. Sanchez-Ruiz, S. Banu Ozkan

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)

TEM-1 β-lactamase evolved from ancestral enzymes that degraded a variety of β-lactam antibiotics with moderate efficiency and degrades β-lactam antibiotics with a strong preference for penicillins. Here authors developed a computational approach t

Externí odkaz: https://doaj.org/article/9f68b130500b474887f6a03663bb2f12

Zobrazit plný text záznamu

Akademický článek

Heme-binding enables allosteric modulation in an ancient TIM-barrel glycosidase

Autor: Gloria Gamiz-Arco, Luis I. Gutierrez-Rus, Valeria A. Risso, Beatriz Ibarra-Molero, Yosuke Hoshino, Dušan Petrović, Jose Justicia, Juan Manuel Cuerva, Adrian Romero-Rivera, Burckhard Seelig, Jose A. Gavira, Shina C. L. Kamerlin, Eric A. Gaucher, Jose M. Sanchez-Ruiz

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)

Family 1 glycosidases (GH1) are present in the three domains of life and share classical TIM-barrel fold. Structural and biochemical analyses of a resurrected ancestral GH1 enzyme reveal heme binding, not known in its modern descendants. Heme rigidif

Externí odkaz: https://doaj.org/article/16437a6b7b904e66bc65cf7320d77917

Zobrazit plný text záznamu

Akademický článek

A protocol to study bacteriophage adaptation to new hosts

Autor: Raquel Luzon-Hidalgo, Valeria A. Risso, Asuncion Delgado, Beatriz Ibarra-Molero, Jose M. Sanchez-Ruiz

Publikováno v: STAR Protocols, Vol 2, Iss 3, Pp 100784- (2021)

Summary: A general protocol for the experimental assessment of bacteriophage adaptation to new hosts is described. We use as a model system the lytic phage T7 and an engineered E. coli strain modified to hamper the recruitment of a known proviral fac

Externí odkaz: https://doaj.org/article/8ac9745717c94e5a83f153721e6e9dea

Zobrazit plný text záznamu

Akademický článek

Efficient Base-Catalyzed Kemp Elimination in an Engineered Ancestral Enzyme

Autor: Luis I. Gutierrez-Rus, Miguel Alcalde, Valeria A. Risso, Jose M. Sanchez-Ruiz

Publikováno v: International Journal of Molecular Sciences, Vol 23, Iss 16, p 8934 (2022)

The routine generation of enzymes with completely new active sites is a major unsolved problem in protein engineering. Advances in this field have thus far been modest, perhaps due, at least in part, to the widespread use of modern natural proteins a

Externí odkaz: https://doaj.org/article/637a3480d49d46e786369640ab828747

Zobrazit plný text záznamu

Plný text ve formátu HTML

Akademický článek

Evidence for a role of phenotypic mutations in virus adaptation

Autor: Raquel Luzon-Hidalgo, Valeria A. Risso, Asuncion Delgado, Eduardo Andrés-León, Beatriz Ibarra-Molero, Jose M. Sanchez-Ruiz

Publikováno v: iScience, Vol 24, Iss 4, Pp 102257- (2021)

Summary: Viruses interact extensively with the host molecular machinery, but the underlying mechanisms are poorly understood. Bacteriophage T7 recruits the small protein thioredoxin of the Escherichia coli host as an essential processivity factor for

Externí odkaz: https://doaj.org/article/ebfa3d63ed2a44cc9ab8f8bd7fcc5edd

Zobrazit plný text záznamu

Akademický článek

Consensus Design of an Evolved High-Redox Potential Laccase

Autor: Bernardo J. Gomez-Fernandez, Valeria A. Risso, Jose M. Sanchez-Ruiz, Miguel Alcalde

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)

Among the broad repertory of protein engineering methods that set out to improve stability, consensus design has proved to be a powerful strategy to stabilize enzymes without compromising their catalytic activity. Here, we have applied an in-house co

Externí odkaz: https://doaj.org/article/69192d5580c04ee0be7655f55b6854ae

Zobrazit plný text záznamu

Akademický článek

De novo active sites for resurrected Precambrian enzymes

Autor: Valeria A. Risso, Sergio Martinez-Rodriguez, Adela M. Candel, Dennis M. Krüger, David Pantoja-Uceda, Mariano Ortega-Muñoz, Francisco Santoyo-Gonzalez, Eric A. Gaucher, Shina C. L. Kamerlin, Marta Bruix, Jose A. Gavira, Jose M. Sanchez-Ruiz

Publikováno v: Nature Communications, Vol 8, Iss 1, Pp 1-13 (2017)

The emergence of novel catalytic functions in ancient proteins likely played a role in the evolution of modern enzymes. Here, the authors use protein sequences from Precambrian beta-lactamases and demonstrate that a single hydrophobic-to-ionizable am

Externí odkaz: https://doaj.org/article/7c76aaa815614d3a948e230f4b0670e6

Zobrazit plný text záznamu

Immunoanalytical Approach for Detecting and Identifying Ancestral Peptide Biomarkers in Early Earth Analogue Environments

Autor: Rita Severino, Mercedes Moreno-Paz, Fernando Puente-Sánchez, Laura Sánchez-García, Valeria A. Risso, Jose M. Sanchez-Ruiz, Nathalie Cabrol, Victor Parro

The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.2c05386. Detailed description of experimental procedures. Illus- tration of antibodies characterization, other FSMI results, inhibition immunoassa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::373617d654c4538e7289f6df677249f1
https://hdl.handle.net/10481/81495

Zobrazit plný text záznamu

Protection of catalytic cofactors by polypeptides as a driver for the emergence of primordial enzymes

Autor: Luis I Gutierrez-Rus, Gloria Gamiz-Arco, J A Gavira, Eric A Gaucher, Valeria A Risso, Jose M Sanchez-Ruiz

Publikováno v: bioRxiv

Enzymes catalyze the chemical reactions of life. For nearly half of known enzymes, catalysis requires the binding of small molecules known as cofactors. Polypeptide-cofactor complexes likely formed at a primordial stage and became starting points for

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eacaab884cd59f647096e180879a420e
https://doi.org/10.1101/2023.03.14.532612

Zobrazit plný text záznamu

Hinge-shift mechanism as a protein design principle for the evolution of β-lactamases from substrate promiscuity to specificity

Autor: Sergio Martínez-Rodríguez, Valeria A. Risso, Tushar Modi, Jose M. Sanchez-Ruiz, Mubark D. Mebrat, Wade D. Van Horn, S. Banu Ozkan, Jose A. Gavira

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Digital.CSIC. Repositorio Institucional del CSIC
instname
Nature Communications
'Nature Communications ', vol: 12, pages: 1852-1-1852-14 (2021)
Digibug: Repositorio Institucional de la Universidad de Granada
Universidad de Granada (UGR)
Digibug. Repositorio Institucional de la Universidad de Granada

W.D.V.H. acknowledges support from National Institutes of Health (Grant: R01GM112077). S.B.O. acknowledges support from the Gordon and Betty Moore Foundations and National Science Foundation (Awards: 1715591 and 1901709). J.M.S.R. acknowledges suppor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d7bb312fe1d0a0603233bca0b3c4873
https://doaj.org/article/9f68b130500b474887f6a03663bb2f12

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání