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pro vyhledávání: '"Valentijn Vrouwe"'
Autor:
Ioannis Mougiakos, Prarthana Mohanraju, Elleke F. Bosma, Valentijn Vrouwe, Max Finger Bou, Mihris I. S. Naduthodi, Alex Gussak, Rudolf B. L. Brinkman, Richard van Kranenburg, John van der Oost
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
CRISPR-Cas9 genome engineering tools have found wide application in a range of species, however they are unsuitable for applications at elevated temperatures. Here the authors characterise ThermoCas9 from which is functional from 20°C to 70°C.
Externí odkaz:
https://doaj.org/article/1d2d2fee1e3d44708bd22d9330e108c2
Autor:
Elleke Fenna Bosma, Richard van Kranenburg, Ioannis Mougiakos, Mihris Ibnu Saleem Naduthodi, Prarthana Mohanraju, John van der Oost, Valentijn Vrouwe, Max Finger Bou, Alex Gussak, Rudolf B. L. Brinkman
Publikováno v:
Nature Communications 8 (2017) 1
Nature Communications
Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
Nature Communications, 8(1)
Mougiakos, I, Mohanraju, P, Bosma, E F, Vrouwe, V, Bou, M F, Naduthodi, M I S, Gussak, A, Brinkman, R B L, van Kranenburg, R & van der Oost, J 2017, ' Characterizing a thermostable Cas9 for bacterial genome editing and silencing ', Nature Communications, vol. 8, 1647 . https://doi.org/10.1038/s41467-017-01591-4
Nature Communications
Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
Nature Communications, 8(1)
Mougiakos, I, Mohanraju, P, Bosma, E F, Vrouwe, V, Bou, M F, Naduthodi, M I S, Gussak, A, Brinkman, R B L, van Kranenburg, R & van der Oost, J 2017, ' Characterizing a thermostable Cas9 for bacterial genome editing and silencing ', Nature Communications, vol. 8, 1647 . https://doi.org/10.1038/s41467-017-01591-4
CRISPR-Cas9-based genome engineering tools have revolutionized fundamental research and biotechnological exploitation of both eukaryotes and prokaryotes. However, the mesophilic nature of the established Cas9 systems does not allow for applications t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::911ad8e3669c59caf0dd2f5d18f8e605
https://research.wur.nl/en/publications/characterizing-a-thermostable-cas9-for-bacterial-genome-editing-a
https://research.wur.nl/en/publications/characterizing-a-thermostable-cas9-for-bacterial-genome-editing-a