Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Valarie L. Tlapak-Simmons"'
Autor:
Valarie L. Tlapak-Simmons, Christina A. Baron, William M. Canfield, Paul H. Weigel, Russell Gotschall, Dewan Haque
Publikováno v:
Journal of Biological Chemistry. 280:13012-13018
Previous studies reached different conclusions about whether class I hyaluronan synthases (HASs) elongate hyaluronic acid (HA) by addition to the reducing or the nonreducing end. Here we used two strategies to determine the direction of HA synthesis
Publikováno v:
Biochemistry. 43:9234-9242
Hyaluronan synthase (HAS) utilizes UDP-GlcUA and UDP-GlcNAc in the presence of Mg(2+) to form the GAG hyaluronan (HA). The purified HAS from Streptococcus equisimilis (seHAS) shows high fidelity in that it only polymerizes the native substrates, UDP-
Autor:
Bruce A. Baggenstoss, Masahiko Endo, Teruzo Miyoshi, Ikuko Kakizaki, Hitoshi Ikeya, Yasufumi Endo, Keiichi Takagaki, Daisuke Kudo, Valarie L. Tlapak-Simmons, Paul H. Weigel, Kshama Kumari, Akio Nakane
Publikováno v:
European Journal of Biochemistry. 269:5066-5075
As observed previously in cultured human skin fibroblasts, a decrease of hyaluronan production was also observed in group C Streptococcus equi FM100 cells treated with 4-methylumbelliferone (MU), although there was no effect on their growth. In this
Publikováno v:
Journal of Biological Chemistry. 273:26100-26109
The functional sizes of the two streptococcal hyaluronan synthases (HASs) were determined by radiation inactivation analysis of isolated membranes. The native enzymes in membranes from Group A Streptococcus pyogenes HAS and Group C Streptococcus equi
Publikováno v:
Biophysical Journal. 75(2):1010-1015
The pH and temperature dependence of the allosteric properties of phosphofructokinase (PFK) from Bacillus stearothermophilus have been studied from 5 to 9 and 6 to 40°C, respectively. Throughout this pH and temperature range the allosteric ligands M
Autor:
Valarie L. Tlapak-Simmons, Bruce A. Baggenstoss, Christina A. Baron, Long Nguyen, Paul H. Weigel, Andria P. Medina
Publikováno v:
Journal of Glycomics & Lipidomics.
Hyaluronan synthase (HAS) uses UDP-GlcUA and UDP-GlcNAc to make hyaluronan (HA). Streptococcus equisimilis HAS (SeHAS) contains four conserved cysteines clustered near the membrane, and requires phospholipids and Mg2+ for activity. Activity of membra
Autor:
Ikuko, Kakizaki, Keiichi, Takagaki, Yasufumi, Endo, Daisuke, Kudo, Hitoshi, Ikeya, Teruzo, Miyoshi, Bruce A, Baggenstoss, Valarie L, Tlapak-Simmons, Kshama, Kumari, Akio, Nakane, Paul H, Weigel, Masahiko, Endo
Publikováno v:
European journal of biochemistry. 269(20)
As observed previously in cultured human skin fibroblasts, a decrease of hyaluronan production was also observed in group C Streptococcus equi FM100 cells treated with 4-methylumbelliferone (MU), although there was no effect on their growth. In this
Publikováno v:
The Journal of biological chemistry. 277(16)
Hyaluronan (HA) synthase (HAS) is a membrane-bound enzyme that utilizes UDP-glucuronic acid (GlcUA) and UDP-GlcNAc to synthesize HA. The HAS from Streptococcus pyogenes (spHAS, 419 amino acids) contains six Cys residues, whereas the enzyme from Strep
Hyaluronan synthase (HAS), the enzyme responsible for the production of hyaluronic acid (HA), is a well-conserved membrane-bound protein in both prokaryotes and eukaryotes. This enzyme performs at least six discrete functions in producing a heterodis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4a744976bad91fcb2e36dcc2f7e6b65
https://europepmc.org/articles/PMC4042541/
https://europepmc.org/articles/PMC4042541/
Autor:
Coy D. Heldermon, Paul H. Weigel, Kshama Kumari, Valarie L. Tlapak-Simmons, Bruce A. Baggenstoss
Publikováno v:
The Journal of biological chemistry. 274(7)
The two hyaluronan synthases (HASs) from Streptococcus pyogenes (spHAS) and Streptococcus equisimilis (seHAS) were expressed in Escherichia coli as recombinant proteins containing His6 tails. The accompanying paper has described the purification and