Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Valérie Guieu"'
Publikováno v:
Analytical Chemistry. 90:4236-4248
Fluorescence polarization/anisotropy is a very popular technique that is widely used in homogeneous-phase immunoassays for the small molecule quantification. In the present Feature, we discuss how the potential of this signaling approach considerably
Autor:
Maxime Henry, Corinne Ravelet, Valérie Guieu, Eric Dausse, Luc Choisnard, Jean-Jacques Toulmé, Guillaume Durand, Eric Peyrin, Laurent Azéma, Emma Goux
Publikováno v:
Nanoscale. 9:4048-4052
Herein, we report a novel approach for the design of a colorimetric aptasensor based on functionalized gold nanoparticle probes. This approach relies on the conjugation of nanoparticles by two functional DNA and RNA hairpins that engage specific kiss
Autor:
Quentin Lespinasse, Valérie Guieu, Emmanuelle Fiore, Emma Goux, Corinne Ravelet, Eric Peyrin, Sandrine Perrier
Publikováno v:
Methods. 97:69-74
This study describes for the first time the feasibility of using peptide nucleic acids (PNAs) as an alternative to the DNA probes in structure-switching aptamer fluorescence polarisation assays. The effects of experimental parameters such as the leng
Autor:
Eric Peyrin, Mohammadali Tabatabaei, Betty C. Galarreta, François Lagugné-Labarthet, Valérie Guieu
Publikováno v:
Analytical and Bioanalytical Chemistry. 405:1613-1621
A selective aptameric sequence is adsorbed on a two-dimensional nanostructured metallic platform optimized for surface-enhanced Raman spectroscopy (SERS) measurements. Using nanofabrication methods, a metallic nanostructure was prepared by electron-b
Publikováno v:
Analytical Chemistry. 84:7203-7211
Here, we describe a new fluorescence polarization aptamer assay (FPAA) strategy which is based on the use of the single-stranded DNA binding (SSB) protein from Escherichia coli as a strong FP signal enhancer tool. This approach relied on the unique a
Publikováno v:
Analytica Chimica Acta. 706:349-353
A label-free, homogeneous aptamer-based sensor strategy was designed for the facile colorimetric detection of small target molecules. The format relied on the target-induced protection of DNA aptamer from the enzymatic digestion and its transduction
Autor:
Corinne Ravelet, Thomas Schmidt, Maroi Mahrous, Sandrine Perrier, Zhenyu Zhu, Eric Peyrin, Valérie Guieu
Publikováno v:
Analytica Chimica Acta. 707:191-196
In this paper, a structure-switching aptamer assay based on a fluorescence polarization (FP) signal transduction approach and dedicated to the L-tyrosinamide sensing was described and optimized. A fluorescently labelled complementary strand (CS) of t
Publikováno v:
Analytical and Bioanalytical Chemistry. 401:3229-3234
A novel fluorescence polarization (FP) aptasensing platform based on target-induced aptamer enzymatic cleavage protection is reported. The method relies on the FP analysis of the phosphodiesterase I mediated size variation of a dye-labeled aptamer. T
Autor:
Petr G. Kuzmin, Robert Carles, Valérie Guieu, G. A. Shafeev, Cosmin Farcau, Sergei V Garnov, Bénédicte Warot-Fontrose, Guillaume Viau, Vladimir V. Bukin
Publikováno v:
The Journal of Physical Chemistry C. 114:15266-15273
Spherical silicon nanoparticles were prepared by laser ablation of a single crystal Si wafer immersed in 95% ethanol with a pulse duration shorter than the time of electron−phonon relaxation (from 35 to 900 fs). The size distribution depends on the
Autor:
Valérie Guieu, Sandrine Perrier, Béatrice Roy, Eric Peyrin, Corinne Ravelet, Christian Périgaud, Jennifer Fize
Publikováno v:
Biosensors and Bioelectronics
Biosensors and Bioelectronics, Elsevier, 2010, 25, pp.1652-1657. ⟨10.1016/j.bios.2009.12.005⟩
Biosensors and Bioelectronics, Elsevier, 2010, 25, pp.1652-1657. ⟨10.1016/j.bios.2009.12.005⟩
A direct fluorescence polarization (FP) assay strategy, dedicated to the small molecule sensing and based on the unique induced-fit binding mechanism of end-labelled nucleic acid aptamers, has been recently developed by our group. Small target bindin