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Manipulating the autolytic pathway of a Bacillus protease

Autor: VandenBurg, B, Eijsink, VGH, Vriend, G, Veltman, OR, Venema, G, HopsuHavu, VK, Jarvinen, M, Kirschke, H

Publikováno v: PROTEOLYSIS IN CELL FUNCTIONS, 120-127
STARTPAGE=120;ENDPAGE=127;TITLE=PROTEOLYSIS IN CELL FUNCTIONS

Autolytic degradation of Bacillus subtilis thermolysin-like proteinase (TLP-sub) is responsible for the irreversible inactivation of the enzyme at elevated temperatures. Previously, we reported five autolysis sites in B. subtilis neutral protease (Va

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=narcis______::3742668aa42940f800ce44b28dc4be00
https://research.rug.nl/en/publications/b89ffe75-e47d-4541-a921-a91e0119d0d9

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INTRODUCTION OF A STABILIZING 10-RESIDUE BETA-HAIRPIN IN BACILLUS-SUBTILIS NEUTRAL PROTEASE

Autor: EIJSINK, VGH, VRIEND, G, VANDENBURG, B, VANDERZEE, [No Value], VELTMAN, OR, STULP, BK, VENEMA, G

Publikováno v: Protein Engineering, 5(2), 157-163

A 10 residue beta-hairpin, which is characteristic of thermostable Bacillus neutral proteases, was engineered into the thermolabile neutral protease of Bacillus subtilis. The recipient enzyme remained fully active after introduction of the loop. Howe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=narcis______::8c66c1f393444cb5c43044ec338b2878
https://research.rug.nl/en/publications/06078fd8-7cf1-4112-897c-47d7a02405df

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STABILIZATION OF THE NEUTRAL PROTEASE OF BACILLUS-STEAROTHERMOPHILUS BY REMOVAL OF A BURIED WATER MOLECULE

Autor: VRIEND, G, BERENDSEN, HJC, VANDERZEE, [No Value], VANDENBURG, B, VENEMA, G, EIJSINK, VGH

Publikováno v: Protein Engineering, 4(8), 941-945

Using site-directed mutagenesis, Ala166 in the neutral protease of Bacillus stearothermophilus was changed into Ser. Model building and molecular dynamics simulations of the mutant enzyme indicated that the Ser hydroxyl group fits well in a cavity wh

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=narcis______::d124d87a38a7daef0cae016248fa4a0e
https://research.rug.nl/en/publications/272cb7ad-7481-47d7-92ea-a70ce34edc4b

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A HIGHLY THERMOSTABLE NEUTRAL PROTEASE FROM BACILLUS-CALDOLYTICUS - CLONING AND EXPRESSION OF THE GENE IN BACILLUS-SUBTILIS AND CHARACTERIZATION OF THE GENE-PRODUCT

Autor: VANDENBURG, B, ENEQUIST, HG, VANDERHAAR, ME, EIJSINK, VGH, STULP, BK, VENEMA, G

Publikováno v: Journal of Bacteriology, 173(13), 4107-4115. AMER SOC MICROBIOLOGY

By using a gene library of Bacillus caldolyticus constructed in phage lambda EMBL12 and selecting for proteolytically active phages on plates supplemented with 0.8% skim milk, chromosomal B. caldolyticus DNA fragments that specified proteolytic activ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=narcis______::93cdd2776ed0783820cfb29bc272fc97
https://research.rug.nl/en/publications/31b43bf8-10f0-4896-bb20-29bc99197a2b

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