Zobrazeno 1 - 10
of 13
pro vyhledávání: '"V. V. Khomov"'
Publikováno v:
Applied Biochemistry and Microbiology. 42:84-88
A simple method of production of total RNA from baker's yeast was developed. Total RNA was isolated from yeast (Saccharomyces cerevisiae) biomass using lysis with sodium dodecyl sulfate at 100 degrees C for 40-60 min and subsequent precipitation of t
Publikováno v:
Doklady Biochemistry and Biophysics. 400:61-64
Publikováno v:
Prikladnaia biokhimiia i mikrobiologiia. 42(1)
A simple method of production of total RNA from baker's yeast was developed. Total RNA was isolated from yeast (Saccharomyces cerevisiae) biomass using lysis with sodium dodecyl sulfate at 100 degrees C for 40-60 min and subsequent precipitation of t
Publikováno v:
Doklady. Biochemistry and biophysics. 401
Autor:
V I, Masycheva, V V, Khomov, A A, Sizov, V A, Razvorotnev, V A, Fadina, E D, Danilenko, L S, Sandakhchiev
Publikováno v:
Doklady Akademii nauk. 344(1)
Publikováno v:
Molekuliarnaia biologiia. 25(2)
Affinity modification of E. coli DNA polymerase I and its Klenow fragment by imidazolides of dNMP (Im-dNMP) and dNTP was studied. DNA polymerase activity of DNA polymerase I was reduced by both Im-dNMP and Im-dNTP. However Im-dNTP does not inactivate
Publikováno v:
Molekuliarnaia biologiia. 24(5)
The Km and vmax values for oligothymidylates d(pT)2-16 in reaction of 3'-5'-exonuclease hydrolysis catalyzed by Klenow fragment were measured in the absence and presence of poly(dA) template without the poly(dA), the Km values for oligonucleotides ar
Autor:
V A, Petrenko, G A, Kuz'micheva, S I, Tat'kov, I I, Krasnoborov, P I, Pozdniakov, A A, Il'ichev, I A, Baturina, V V, Khomov
Publikováno v:
Molekuliarnaia biologiia. 24(2)
The mutation system has been suggested in an effort to test insertion and deletion mutants by changing the Lac-phenotype of bacterial colonies transformed by mutant DNA. This system also makes possible to determine heterozygotes and homozygotes among
Publikováno v:
Molekuliarnaia biologiia. 24(1)
The comparison of the Km and Vmax values for the primers was carried out. The primers were either completely complementary to the template or contained non-complementary bases at different positions with respect to the 3'-end. The addition of NaF, se
Publikováno v:
Molekuliarnaia biologiia. 24(1)
AMP and NaF each taken separately were shown to activate DNA polymerization catalyzed by Klenow fragment of DNA polymerase I by means of interaction of AMP or NaF with 3'----5'-exonuclease center of the enzyme. In the presence of NaF which is a selec