Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples

Autor: G. Papadakis, A. K. Pantazis, N. Fikas, S. Chatziioannidou, V. Tsiakalou, K. Michaelidou, V. Pogka, M. Megariti, M. Vardaki, K. Giarentis, J. Heaney, E. Nastouli, T. Karamitros, A. Mentis, A. Zafiropoulos, G. Sourvinos, S. Agelaki, E. Gizeli

Publikováno v: Scientific Reports, Vol 12, Iss 1, Pp 1-15 (2022)

Abstract Loop-mediated isothermal amplification is known for its high sensitivity, specificity and tolerance to inhibiting-substances. In this work, we developed a device for performing real-time colorimetric LAMP combining the accuracy of lab-based

Externí odkaz: https://doaj.org/article/27ccdcb6d5a34cb0a8674a3badbfe846

Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples

Autor: Georgios Z. Papadakis, M. Vardaki, A. K. Pantazis, K. Giarentis, Sophia Agelaki, Kleita Michaelidou, S. Chatziioannidou, Electra Gizeli, A. Zafiropoulos, Judith Heaney, M. Megariti, Vasiliki Pogka, Eleni Nastouli, George Sourvinos, Timokratis Karamitros, V. Tsiakalou, Nikolaos Fikas, Alexios-Fotios A. Mentis

Loop-mediated isothermal amplification is known for its high sensitivity, specificity and tolerance to inhibiting-substances. We developed a device for performing real-time colorimetric LAMP combining the accuracy of lab-based quantitative molecular

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::52873407fcd5b0e438a409e275de0ac4
https://doi.org/10.1101/2020.07.22.215251

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Evaluation of a near-patient SARS-CoV-2 novel rapid diagnostic platform.

Autor: Botha JC; Department of Infection, Immunity and Inflammation, Institute of Child Health, University College London, London, United Kingdom.; University College London Hospitals NHS Trust, Advanced Pathogen Diagnostics Unit, London, United Kingdom., Zafilaza K; Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique, AP-HP, Hôpitaux Universitaires Pitié Salpêtrière-Charles Foix, Laboratoire de Virologie, Paris, France., Soulie C; Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique, AP-HP, Hôpitaux Universitaires Pitié Salpêtrière-Charles Foix, Laboratoire de Virologie, Paris, France., Yin N; Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), Université Libre de Bruxelles, Brussels, Belgium., Spyer M; Department of Infection, Immunity and Inflammation, Institute of Child Health, University College London, London, United Kingdom., Balaska S; Department of Microbiology, AHEPA University Hospital, Medical School Aristotle University of Thessaloniki, Thessaloniki, Greece., Chatziioannidou S; BIOPIX DNA TECHNOLOGY PC, Science and Technology Park of Crete, Heraklion, Greece., Tsiakalou V; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece., Papadakis G; BIOPIX DNA TECHNOLOGY PC, Science and Technology Park of Crete, Heraklion, Greece., Skoura L; Department of Microbiology, AHEPA University Hospital, Medical School Aristotle University of Thessaloniki, Thessaloniki, Greece., Zafiropoulos A; Laboratory of Clinical Virology, School of Medicine, University of Crete, Heraklion, Greece., Sourvinos G; Laboratory of Clinical Virology, School of Medicine, University of Crete, Heraklion, Greece., Vandenberg O; Research and Technology Innovation Unit, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), Université Libre de Bruxelles, Brussels, Belgium., Marcelin A-G; Sorbonne Université, INSERM, Institut Pierre Louis d'Epidémiologie et de Santé Publique, AP-HP, Hôpitaux Universitaires Pitié Salpêtrière-Charles Foix, Laboratoire de Virologie, Paris, France., Gizeli E; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece.; Department of Biology, University of Crete, Heraklion, Greece., Nastouli E; Department of Infection, Immunity and Inflammation, Institute of Child Health, University College London, London, United Kingdom.; University College London Hospitals NHS Trust, Advanced Pathogen Diagnostics Unit, London, United Kingdom.

Publikováno v: Microbiology spectrum [Microbiol Spectr] 2024 Oct 18, pp. e0067224. Date of Electronic Publication: 2024 Oct 18.