Zobrazeno 1 - 10
of 71
pro vyhledávání: '"V. P. Shelepov"'
Autor:
I. V. Botezatu, I. O. Panchuk, A. A. Kolomeytseva, A. A. Fedenko, N. N. Mazurenko, I. V. Tsyganova, O. Yu. Susova, V. N. Kondratova, V. P. Shelepov, A. V. Lichtenstein
Publikováno v:
Успехи молекулярной онкологии, Vol 5, Iss 1, Pp 35-42 (2018)
Objective: to evaluate the possibility of using a highly sensitive method of “enriched” polymerase chain reaction followed by DNA melting analysis (PCR-DMA) for target liquid biopsy of cancer patients.Materials and methods. The “enriched” PCR
Externí odkaz:
https://doaj.org/article/c36a853db30e43719f641e0ebe646d81
Publikováno v:
Успехи молекулярной онкологии, Vol 4, Iss 1, Pp 46-52 (2017)
Polymerase chain reaction (PCR) followed by DNA melting analysis with TaqMan probes effectively reveals mutations in the human genome “hot” spots. The necessity to carry out PCR in the asymmetric variant causes, however, a number of restrictions
Externí odkaz:
https://doaj.org/article/9c127f68d9e04a8881d1b8d9bd37806b
Autor:
I. V. Botezatu, I. O. Panchuk, A. M. Stroganova, A. I. Senderovich, V. N. Kondratova, V. P. Shelepov, A. V. Liсhtenshtein
Publikováno v:
Успехи молекулярной онкологии, Vol 3, Iss 2, Pp 42-49 (2016)
DNA Melting Analysis is very effective in clinical DNA diagnostics: it is simple to perform, high throughput, labor-, time- and cost-effective and is implemented in the “closed tube” format minimizing the risk of samples cross-contamination. Alth
Externí odkaz:
https://doaj.org/article/9d0c955ca7ff40aeb36d3939c53a28fc
Publikováno v:
Uspehi Molekulârnoj Onkologii, Vol 4, Iss 1, Pp 46-52 (2017)
Polymerase chain reaction (PCR) followed by DNA melting analysis with TaqMan probes effectively reveals mutations in the human genome “hot” spots. The necessity to carry out PCR in the asymmetric variant causes, however, a number of restrictions
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f2e9aa469f6f1a360d6332e1dbab407
https://umo.abvpress.ru/jour/article/view/88
https://umo.abvpress.ru/jour/article/view/88
Autor:
Valentina N. Kondratova, Anna Stroganova, Anastasia I. Senderovich, Irina O Panchuk, V. P. Shelepov, I. V. Botezatu, Anatoly V. Lichtenstein
Publikováno v:
BioTechniques. 62:62-68
Asymmetric PCR and DNA melting analysis with TaqMan probes applied for mutation detection is effectively used in clinical diagnostics. The method is simple, cost-effective, and carried out in a closed-tube format, minimizing time, labor, and risk of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::176b44a780213b6ff538acf551af51d5
https://doi.org/10.2144/000114515
https://doi.org/10.2144/000114515
Autor:
I. O. Panchuk, V. P. Shelepov, A. M. Stroganova, I. V. Botezatu, A. V. Lichtenstein, Valentina N. Kondratova, A. I. Senderovich
Publikováno v:
Molecular Biology. 51:41-48
Scanning for mutations by DNA melting analysis (DMA) is based on asymmetric PCR followed by the melting of duplexes formed by single-stranded amplicons with TaqMan probes. The method is optimally suited for clinical genetic testing; it is easy to per
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ede8b42a7374c75362f93679c2cc4376
https://doi.org/10.1134/s002689331701006x
https://doi.org/10.1134/s002689331701006x
Autor:
V. P. Shelepov, Anatoly V. Lichtenstein, Natalia N. Mazurenko, Valentina N. Kondratova, Olga Y. Susova, I. V. Botezatu, Irina V. Tsyganova
Publikováno v:
Analytical biochemistry. 590
Identification of mutant genes in tumor tissues and blood plasma (solid and liquid biopsy samples, respectively) is a necessity for individualized treatment of cancer patients. Here we report the use of a novel mutant-enriched PCR - quantitative DNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d761e2b28a4e3a983ff3f0101b6a1788
https://pubmed.ncbi.nlm.nih.gov/31782995
https://pubmed.ncbi.nlm.nih.gov/31782995
Autor:
I. V. Botezatu, Irina O. Nechaeva, Аnna М. Stroganova, V. P. Shelepov, Valentina N. Kondratova, Anastasia I. Senderovich, Anatoly V. Lichtenstein
Publikováno v:
Data in Brief
Data in Brief, Vol 5, Iss C, Pp 913-917 (2015)
Data in Brief, Vol 5, Iss C, Pp 913-917 (2015)
The data in this article are related to the research article entitled “Optimization of melting analysis with TaqMan probes for detection of KRAS, NRAS, and BRAF mutations” Botezatu et al. [1]. Somatic mutations in the PIK3CA gene (“hot spots”
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9967136769985747b15546167dbc515
http://europepmc.org/articles/PMC4669479
http://europepmc.org/articles/PMC4669479
Publikováno v:
Molecular Biology. 48:878-885
Recent studies of human and animal tumor tissues revealed a high transcriptional activity of pericentromeric satellite DNA repeats; i.e., they were found to produce up to half of all transcripts in tumor cells, which is many times more than in normal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::52115ef2b6663f52660f23346a44e194
https://doi.org/10.1134/s0026893314060089
https://doi.org/10.1134/s0026893314060089
Autor:
I V, Botezatu, I O, Panchuk, A M, Stroganova, A I, Senderovich, V N, Kondratova, V P, Shelepov, A V, Lichtenstein
Publikováno v:
Molekuliarnaia biologiia. 51(1)
Scanning for mutations by DNA melting analysis (DMA) is based on asymmetric PCR followed by the melting of duplexes formed by single-stranded amplicons with TaqMan probes. The method is optimally suited for clinical genetic testing; it is easy to per
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::fe0ee66538330c3ae8938cd628ffb2b8
https://pubmed.ncbi.nlm.nih.gov/28251966
https://pubmed.ncbi.nlm.nih.gov/28251966