Zobrazeno 1 - 10
of 52
pro vyhledávání: '"V. N. Dobrynin"'
Autor:
V. N. Dobrynin, I. A. Filozova
Publikováno v:
Компьютерные исследования и моделирование, Vol 7, Iss 3, Pp 661-673 (2015)
The article discusses the approach to the improvement of information processing technology on the basis of logical-semantic network (LSN) Question-Answer-Reaction aimed at formation and support of the catalog service providing efficient search of ans
Externí odkaz:
https://doaj.org/article/0f21f1cdfd354d0581b6621afa63ed6e
Autor:
I. A. Filozova, V. N. Dobrynin
Publikováno v:
Компьютерные исследования и моделирование, Vol 7, Iss 3, Pp 661-673 (2015)
The article discusses the approach to the improvement of information processing technology on the basis of logical-semantic network (LSN) Question-Answer-Reaction aimed at formation and support of the catalog service providing efficient search of ans
Autor:
Olga N. Danilevskaya, Oleg N. Voloshin, A. G. Veselkov, V. N. Dobrynin, Maria N. Pavlova, Boris P. Belotserkovskii, Maxim D. Frank-Kamenetskii
Publikováno v:
Journal of Biomolecular Structure and Dynamics. 9:643-652
We have found, with the aid of 2-D gel electrophoresis, that double-stranded human telomeric repeat, (T2AG3)12·(C3TA2)12, being cloned within a plasmid, forms a protonated superhelically-induced structure. Experiments on chemical and enzymatic probi
Autor:
Maxim D. Frank-Kamenetskii, Sergei M. Mirkin, S. A. Filippov, Boris P. Belotserkovskii, A. G. Veselkov, V. N. Dobrynin
Publikováno v:
Nucleic Acids Research. 18:6621-6624
We have used two-dimensional gel electrophoresis to study the structural transition to the triplex H form of sequences 5'-AAGGGAGAAXGGGGTATAGGGGYAAGAGGGAA-3' where X and Y are any DNA bases. The transition was observed at acid pH under superhelical s
Publikováno v:
Bioorganicheskaia khimiia. 20(8-9)
A streptavidin-coated TSK-gel support, with the loading capacity of 50-70 nmol of biotinylated substance per gram of dry support, and biotinylated oligonucleotides, containing the 4,9-dithiadodecane-6,7-dihydroxy-1,12-diphosphate insert, were prepare
Autor:
D K, Pokholok, S O, Gudima, D S, Esipov, V N, Dobrynin, L V, Memelova, V O, Rechinskiĭ, S N, Kochetkov
Publikováno v:
Biokhimiia (Moscow, Russia). 59(5)
Prolonged therapy of AIDS patients with azidothymidine results in the development of resistance to the drug. This phenomenon is accompanied by the appearance of point mutations in the pol gene coding for reverse transcriptase (RT). Kinetic studies of
Autor:
Sergey N. Kochetkov, D K Pokholok, Vladimir O. Rechinsky, S. O. Gudima, Dmitry S. Yesipov, V. N. Dobrynin
Publikováno v:
FEBS letters. 325(3)
To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were com
Autor:
V G, Korobko, I V, Davydov, V N, Dobrynin, N M, Pustoshilova, L R, Lebedev, I P, Gileva, V A, Petrenko
Publikováno v:
Bioorganicheskaia khimiia. 19(4)
Using the oligonucleotide directed mutagenesis, a human lymphotoxin (TNF beta) mutant gene lacking 21 N-terminal codons has been obtained. Recombinant plasmid pLT21 for expression of the mutant gene has been constructed. The mutant gene in the plasmi
Publikováno v:
Bioorganicheskaia khimiia. 18(1)
Gene estA coding for thermostable enterotoxin of Escherichia coli has been cloned. It is shown that in the E. coli strain SA162 this gene is located on the chromosome. Using polymerase chain reaction a site-directed mutagenesis of the cloned gene has
Publikováno v:
Bioorganicheskaia khimiia. 17(10)
The cDNA sequence for human tumor necrosis factor (hTNF) was reconstructed in vitro from genomic sequence. Using the oligonucleotide directed mutagenesis, a site for restriction endonuclease ClaI was introduced into the end of the first exon. The nuc