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Akademický článek
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Publikováno v:
Heart, Lung and Circulation. 30:S14
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1bd85c4c8e5e57bf8e2b5d2bd7c21a15
https://doi.org/10.1016/j.hlc.2021.03.043
https://doi.org/10.1016/j.hlc.2021.03.043
Publikováno v:
Journal of Chromatography B. 808:111-115
The aim of this work was to test a chromatographic support, 4-mercaptoethyl pyridine (4-MEP) Hypercel, for penicillin acylase purification by using pure penicillin acylase and crude extract. Two equilibration buffers with various salt concentrations
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e2c0e7270236a5cface995fa763f058
https://doi.org/10.1016/j.jchromb.2004.03.025
https://doi.org/10.1016/j.jchromb.2004.03.025
Publikováno v:
Journal of Biochemical and Biophysical Methods. 56:69-78
The aim of this work was to test a new matrix for group size exclusion chromatography, Matrex cellufine GH 25, and compare it with Sephadex G25 Superfine and Sephadex G25 fine. Matrex cellufine GH 25 showed a better behaviour at high flow rate (792 c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9b6d527aa817df05486a3c555783fb6
https://doi.org/10.1016/s0165-022x(03)00073-3
https://doi.org/10.1016/s0165-022x(03)00073-3
Publikováno v:
Journal of biochemical and biophysical methods. 49(1-3)
The introduction of affinity chromatography has opened a new dimension in protein purification. This article reviews the current techniques used in the penicillin acylase purification process, especially pseudo-affinity chromatography. A profile for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::469aa36f63432257b02c51a34ddd94db
https://pubmed.ncbi.nlm.nih.gov/11694301
https://pubmed.ncbi.nlm.nih.gov/11694301
Autor:
Xavier Santarelli, V. Fitton
Publikováno v:
Journal of chromatography. B, Biomedical sciences and applications. 754(1)
The aim of this work was to test immobilized metal affinity chromatography (IMAC) for the purification of penicillin acylase. After evaluation of different metals, Cu2+ was selected. Different samples were tested: pure penicillin acylase, industrial
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d9646c33d8d9eb9d9fa2853f3df0ef4
https://pubmed.ncbi.nlm.nih.gov/11318408
https://pubmed.ncbi.nlm.nih.gov/11318408
Publikováno v:
Journal of chromatography. B, Biomedical sciences and applications. 753(1)
A two-step chromatographic purification procedure from clarified Escherichia coli ultrasonic homogenate was evaluated. The capture step included immobilized metal affinity chromatography with Cu2+ as metal ion. Two elution methods were performed: 1 M
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d2d0d6830bfe9807754185d087b6e62
https://pubmed.ncbi.nlm.nih.gov/11302447
https://pubmed.ncbi.nlm.nih.gov/11302447
Publikováno v:
Journal of chromatography. B, Biomedical sciences and applications. 739(1)
New pseudo-affinity chromatographic supports for penicillin acylase were prepared and evaluated with three different samples: pure penicillin acylase, industrial clarified feedstock and crude extract.The different gels were studied for their purifica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::369f6627b81eb6321b6e09b91dd60225
https://pubmed.ncbi.nlm.nih.gov/10744314
https://pubmed.ncbi.nlm.nih.gov/10744314
Publikováno v:
Molecular and cellular biochemistry. 184(1-2)
In this work, we first compared yeast mitochondrial oxidative metabolism at different levels of organization: whole cells (C), spheroplasts (S), permeabilized spheroplasts (PS) or isolated mitochondria (M). At present, S are more suitable for use tha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::002400922c41b3e77d5966a4bc254ede
https://pubmed.ncbi.nlm.nih.gov/9746313
https://pubmed.ncbi.nlm.nih.gov/9746313