Zobrazeno 1 - 10
of 14
pro vyhledávání: '"V. Degouys"'
Publikováno v:
Cytotechnology. 19:107-110
The lengthy and cumbersome protocol used to establish the growth kinetics characteristics of anchorage-dependent cells (ADC's)in situ (i.e. while the cells adhere on their microsupport) by Aperture Impedance Pulse Spectroscopy (AIPS) can be replaced
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b395688cec32405338e6e090656b487
https://doi.org/10.1007/bf00749765
https://doi.org/10.1007/bf00749765
Autor:
C. Montoisy, R. K. Togasaki, M.-P. Wanderpepen, A. O. A. Miller, V. Ségault, V. Degouys, S. J. Surzycki
Publikováno v:
Animal Cell Technology ISBN: 9789401062732
HeLa cell nuclei, the reference source for the isolation of factors involved in reactions such as initiation of transcription, splicing, polyadenylation, are obtained following Dounce homogenization of hypotonically fragilized cells{(1, 2, 3).
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::77be3b872fcd2bc5d3e68b1b6447cc5d
https://doi.org/10.1007/978-94-011-5404-8_42
https://doi.org/10.1007/978-94-011-5404-8_42
Publikováno v:
Animal Cell Technology ISBN: 9789401062732
Capacitance measurement performed at 1MHz have been used to on-line, non invasively monitor and possibly control the biomass of long term high density perfused HeLa cells suspension cultures. Perfusion is realized by the combined action of a spin fil
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8c73b03d2ae8d6ad74d2357ff6b9a2b8
https://doi.org/10.1007/978-94-011-5404-8_38
https://doi.org/10.1007/978-94-011-5404-8_38
Autor:
V. Degouys, D. A. Dubois
Publikováno v:
Animal Cell Technology: Developments Towards the 21st Century ISBN: 9789401041959
Continuous production of biologicals from high-density cultures requires that cell physiology be maintained in high-performance conditions for long periods. In the present paper, cell culture experiments are presented, where the bioreactor is kept in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5d84d40edf3f20dffb291157c9df036a
https://doi.org/10.1007/978-94-011-0437-1_25
https://doi.org/10.1007/978-94-011-0437-1_25
Besides the possibilities offered by dielectric spectroscopy to determine the cell size and the biomass of isolated cells growing in suspension as well as to in situ (i.e. without prior trypsinisation) perform the same determinations on anchorage-dep
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9a55636068f2017355d70b91244f2e75
https://doi.org/10.1016/b978-0-7506-1845-8.50062-4
https://doi.org/10.1016/b978-0-7506-1845-8.50062-4
Publikováno v:
Cytotechnology. 13(3)
The capacitance of suspensions of CHO and HeLa cells (0.5-3 x 10(6) cells/ml) has been measured between 0.2 and 10 MHz. As frequencies decrease, there is a continuous increase in capacitance of both the cell suspension and the spent growth medium fre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f4d1dafc49568667374a0882ad61abe
https://pubmed.ncbi.nlm.nih.gov/7764661
https://pubmed.ncbi.nlm.nih.gov/7764661
Publikováno v:
Cytotechnology. 13(3)
Low-frequency dielectric spectroscopy has been used in situ, i.e. while the cells are still attached to their microsupport, to monitor the changes of biomass accompanying the growth of anchorage-dependent cells. This method, when compared to Aperture
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64eafe305864e463dcace351ceddaa66
https://pubmed.ncbi.nlm.nih.gov/7513180
https://pubmed.ncbi.nlm.nih.gov/7513180
Akademický článek
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.
K zobrazení výsledku je třeba se přihlásit.
Akademický článek
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.
K zobrazení výsledku je třeba se přihlásit.
Akademický článek
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.
K zobrazení výsledku je třeba se přihlásit.