Výsledky vyhledávání - "V. A. Vasiliskov"

The Efficacy of Hybridization Analysis of DNA Labeled with Red and Near-Infrared Cyanine Dyes

Autor: Alexander V. Chudinov, Sergey A. Lapa, V. E. Kuznetsova, Yu. V. Moroz, T. V. Nasedkina, A. Yu. Ikonnikova, V. E. Shershov, R. A. Miftakhov, V. A. Vasiliskov

Publikováno v: Biophysics. 66:25-31

We present a study of the sensitivity of a hybridization microarray analysis of DNA labeled with 2'‑deoxyuridine-5'-triphosphate derivatives that contain the Cy5 and Cy7 cyanine fluorophore dyes. Two Cy5-dUTP and two Cy7-dUTP derivatives that can b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e44a30db13993ae889548c084d25a08f
https://doi.org/10.1134/s000635092101005x

Zobrazit plný text záznamu

Mononucleotide repeat expansions with non-natural polymerase substrates

Autor: Alexander V. Chudinov, Sergey A. Lapa, V. E. Kuznetsova, Natalia A. Kolganova, V. A. Vasiliskov, Edward N. Timofeev

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
Scientific Reports

Replicative strand slippage is a biological phenomenon, ubiquitous among different organisms. However, slippage events are also relevant to non-natural replication models utilizing synthetic polymerase substrates. Strand slippage may notably affect t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88159c9022a857b088bf16007f68fbb5
https://doaj.org/article/7b73fccda1b24e75804200257fc598d1

Zobrazit plný text záznamu

Plný text ve formátu HTML

The Efficiency of DNA Labeling with Near-Infrared Fluorescent Dyes

Autor: V. E. Kuznetsova, T. V. Nasedkina, V. A. Vasiliskov, Alexander V. Chudinov, V. E. Shershov, A. Yu. Ikonnikova, Sergey A. Lapa, R. A. Miftakhov

Publikováno v: Biophysics. 65:736-741

The efficiency of DNA labeling was assessed for 2'-deoxyuridine 5'-triphosphate (dUTP) derivatives containing the Cy7 cyanine dye as a fluorophore. Two fluorescent Cy7-labeled dUTP analogs differed in the chemical structure of the linker between the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7cfd2b20074171b7305918cdd009b14c
https://doi.org/10.1134/s0006350920050188

Zobrazit plný text záznamu

Slippage of the Primer Strand in the Primer Extension Reaction with Modified 2'-Deoxyuridine Triphosphates

Autor: V. A. Vasiliskov, Edward N. Timofeev, V. E. Kuznetsova, A. V. Lisitsa, Sergey Surzhikov, S. P. Radko, Alexander S. Zasedatelev, Sergey A. Lapa, R. A. Miftahov, V. E. Shershov, Alexander V. Chudinov

Publikováno v: Russian Journal of Bioorganic Chemistry. 46:312-314

An effect of primer strand slippage was observed in the primer extension reaction due to replacement of thymidine triphosphate for modified analogs of dUTP. The effect was detected when using Vent (exo–) DNA polymerase. It is notably suppressed by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::13f4f8e5496396abb6746ebd8db39afa
https://doi.org/10.1134/s106816202003022x

Zobrazit plný text záznamu

Novel 5-Alkylcarboxamide-2'-Deoxyuridine-5'-Triphosphates for Enzymatic Synthesis of Highly Modified DNA

Autor: T. O. Guseinov, Alexander V. Chudinov, O. A. Zasedateleva, S. P. Radko, R. A. Miftahov, Edward N. Timofeev, A. V. Lisitsa, Sergey Surzhikov, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Kuznetsova, V. E. Shershov, M. A. Spitsyn, V. A. Vasiliskov

Publikováno v: Russian Journal of Bioorganic Chemistry. 45:221-223

The synthesis of deoxyuridine triphosphate derivatives with alkyl groups of various chain length attached to the pyrimidine base by the trans-alkene linker was performed. The efficiency of the incorporation of modified nucleotides during the synthesi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::70f5e0d19cdde486902c3019e30aabe3
https://doi.org/10.1134/s1068162019030063

Zobrazit plný text záznamu

Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases

Autor: Natalia A. Kolganova, Igor P. Smirnov, V. A. Vasiliskov, Edward N. Timofeev, Alexander V. Chudinov

Publikováno v: Scientific Reports
Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)

Non-natural nucleotide substrates are widely used in the enzymatic synthesis of modified DNA. The terminal activity of polymerases in the presence of modified nucleotides is an important, but poorly characterized, aspect of enzymatic DNA synthesis. H

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12a4329ba934d61f0e9eda9f1df18826
http://europepmc.org/articles/PMC5532294

Zobrazit plný text záznamu

Bridge DNA amplification of cancer-associated genes on cross-linked agarose microbeads

Autor: T. V. Nasedkina, D. O. Fesenko, Edward N. Timofeev, Natalia A. Kolganova, Alexander V. Chudinov, V. E. Kuznetsova, V. A. Vasiliskov, Anton E. Egorov, Alexander S. Zasedatelev

Publikováno v: Microchimica Acta. 182:557-563

A cross-linked agarose substrate was studied as a 3D support for bridge solid-phase DNA amplification (SPA). In this kind of SPA, primers are immobilized on agarose beads. Flow cell studies of SPA in real-time experiments showed that the amplificatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::328d8d6e1a102ade22277a85e7471a78
https://doi.org/10.1007/s00604-014-1357-8

Zobrazit plný text záznamu

The effect of chromophore charge on the incorporation efficiency of fluorescence-labeled nucleotides catalyzed by Taq DNA polymerase in matrix synthesis

Autor: Yu. P. Lysov, V. E. Barsky, Alexander S. Zasedatelev, V. E. Kuznetsova, Sergey Surzhikov, Alexander V. Chudinov, V. E. Shershov, V. A. Vasiliskov, O. A. Zasedateleva, T. O. Guseinov, M. A. Spitsyn

Publikováno v: Biophysics. 60:1013-1015

The effect of the chromophore charge on the efficiency of incorporation of fluorescent-labeled nucleotides into DNA during PCR was studied using three dUTP derivatives that contain different fluorescent labels, i.e., electroneutral, positively charge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::13ca0d8da30343e95acdb3dc3748e020
https://doi.org/10.1134/s0006350915060238

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání