Zobrazeno 1 - 10
of 52
pro vyhledávání: '"V. Lipkin"'
Autor:
Konstantin M. Boyko, Timur N. Baymukhametov, Yury M. Chesnokov, Michael Hons, Sofya V. Lushchekina, Alexey V. Lipkin, Vladimir O. Popov, Michail V. Kovalchuk
Publikováno v:
International Journal of Biomedicine, Vol 9, Iss Suppl_1, Pp S14-S15 (2019)
Background: Human plasma butyrylcholinesterase (hBChE, EC 3.1.1.8) is an endogenous bioscavenger that hydrolyzes numerous medicamentous and poisonous esters and scavenges potent organophosphorus nerve agents. Thus, the hBChE can be used to protect ac
Externí odkaz:
https://doaj.org/article/1a510888dc1f4c52882f5f7e2cca0975
Publikováno v:
Applied Biochemistry and Microbiology. 57:297-302
A producer strain of Komagataella phaffii of recombinant beluga whale (Delphinapterus leucas) chymosin with a productivity of 80 mg/L was obtained, and the physico-chemical properties of the enzyme were studied for possible use in the food industry.
Autor:
A. N. Fedorov, E. A. Vavilova, A. V. Lipkin, N. V. Chertova, E. G. Sadykhov, M. A. Eldarov, S. S. Zatsepin, S. Y. Filkin
Publikováno v:
Applied Biochemistry and Microbiology. 56:657-661
An effective recombinant strain of Komagataella phaffii for Bos taurus prochymosin production was obtained. A method for the isolation and purification of recombinant chymosin that includes two-stage purification via ion exchange and hydrophobic chro
Autor:
S. Y. Filkin, E. G. Sadykhov, A. A. Zenin, A N Fedorov, Vladimir Popov, A. V. Lipkin, A A Sichev, D S Bityak, N. V. Chertova
Publikováno v:
Preparative Biochemistry & Biotechnology. 50:549-555
Aim: The main purpose of this work was to develop new protocols for high yield purification of secretory phospholipase A2 (PLA2) and to investigate its biophysical properties.Materials and methods:...
Publikováno v:
Biochemistry (Moscow). 85:177-195
Enzymes of the phospholipase superfamily are involved in lipid metabolism, as well as regulation of membrane composition, cell signaling, and inflammation. This review provides an insight into the structure, functional properties, and biotechnologica
Publikováno v:
Applied Biochemistry and Microbiology. 55:670-676
An efficient recombinant strain of the methylotrophic yeast Pichia pastoris was obtained; it produces active phospholipase A2 (PLA2) in a high yield. As a result of three stages of purification, a preparation of ~90% purity was obtained. The results
Autor:
D. A. Korzhenevskii, A. Yu. Nikolaeva, A. V. Lipkin, Vladimir I. Timofeev, K. M. Boiko, Tatiana V. Rakitina, Pavel V. Dorovatovskii
Publikováno v:
Crystallography Reports. 60:880-883
HU proteins are involved in bacterial DNA and RNA repair. Since these proteins are absent in cells of higher organisms, inhibitors of HU proteins can be used as effective and safe antibiotics. The crystallization conditions for the M. gallisepticum H
Publikováno v:
Crystallography Reports. 58:442-449
The Protein Factory was established at the Centre for Nano, Bio, Info, Cognitive, and Social Sciences and Technologies (NBICS Centre) of the National Research Centre “Kurchatov Institute” in 2010. The Protein Factory, together with the Centre for
Autor:
A. G. Yarosh, I.A. Shumilin, K. M. Boiko, M.A. Gorbacheva, A. V. Lipkin, D. A. Korzhenevskii, Vladimir Popov, Pavel V. Dorovatovskii, Tatiana V. Rakitina
Publikováno v:
Russian Journal of Bioorganic Chemistry. 38:83-88
Three proteins from extremophilic bacteria—hypothetical monooxygenase from Deinococcus radiodurans, hypothetical nucleotidyl transferase from Thermotoga maritime, and hypothetical oxidoreductase from Exiguobacterium sibiricum—and the DJ-1 chapero
Publikováno v:
Acta Naturae. 1:84-88
the discovery of the pharmaceutical potential of small molecule inhibitors of oncogenic protein tyrosine kinases is one of the directions in target therapy in oncology. Presently, investigations aiming at developing new therapeutically important inhi