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The role of the small regulatory RNA GcvB in GcvB/mRNA posttranscriptional regulation of oppA and dppA in Escherichia coli

Autor: Sarah C. Pulvermacher, George V. Stauffer, Lorraine T. Stauffer

Publikováno v: FEMS Microbiology Letters. 281:42-50

The gcvB gene encodes two small, nontranslated RNAs that regulate OppA and DppA, periplasmic binding proteins for the oligopeptide and dipeptide transport systems. Analysis of the gcvB sequence identified a region of complementarity near the ribosome

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6fa6be48933d559d057de24d3e479371
https://doi.org/10.1111/j.1574-6968.2008.01068.x

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Adsorption of DOPC onto Hg from the G∣S interface and from a liposomal suspension

Autor: V Stauffer, Dan Bizzotto, Robin Stoodley, J.O. Agak

Publikováno v: Journal of Electroanalytical Chemistry. 516:73-82

The adsorption of various physical forms of dioleoyl phosphatidylcholine (DOPC) onto a Hg electrode have been studied. The three systems investigated were adsorption of DOPC from the monolayer state present on the gas ∣ solution (G ∣ S) interface

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::80bd45b6a68673f1efe0fcb1e18a7c03
https://doi.org/10.1016/s0022-0728(01)00655-6

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GcvR interacts with GcvA to inhibit activation of the Escherichia coli glycine cleavage operon

Autor: Angela C. Ghrist, Gary Heil, George V. Stauffer

Publikováno v: Microbiology. 147:2215-2221

The Escherichia coli glycine cleavage enzyme system, encoded by the gcvTHP operon, catalyses the oxidative cleavage of glycine to CO(2), NH(3) and a one-carbon methylene group. Transcription of the gcv operon is positively regulated by GcvA and negat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6f1f7561aa53a53241832f2e65287aa
https://doi.org/10.1099/00221287-147-8-2215

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GcvA binding site 1 in the gcvTHP promoter of Escherichia coli is required for GcvA-mediated repression but not for GcvA-mediated activation

Autor: George V. Stauffer, Laura D. Wonderling, Mark L. Urbanowski

Publikováno v: Microbiology. 146:2909-2918

GcvA binds to three sites in the gcvTHP control region, from base -34 to -69 (site 1), from base -214 to -241 (site 2) and from base -242 to -271 (site 3). Previous results suggested that sites 3 and 2 are required for both GcvA-dependent activation

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4da0fcbd5883993c8ace4bbf82e30fa9
https://doi.org/10.1099/00221287-146-11-2909

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Role of the RNA Polymerase α Subunits in MetR-Dependent Activation of metE and metH : Important Residues in the C-Terminal Domain and Orientation Requirements within RNA Polymerase

Autor: Paula S. Fritsch, George V. Stauffer, Mark L. Urbanowski

Publikováno v: Journal of Bacteriology. 182:5539-5550

Many transcription factors activate by directly interacting with RNA polymerase (RNAP). The C terminus of the RNAP α subunit (αCTD) is a common target of activators. We used both random mutagenesis and alanine scanning to identify αCTD residues th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cbe04f28c0e4ee59b7149b0f44f1bf8
https://doi.org/10.1128/jb.182.19.5539-5550.2000

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ThegcvBgene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems inEscherichia coli

Autor: Lorraine T. Stauffer, George V. Stauffer, Mark L. Urbanowski

Publikováno v: Molecular Microbiology. 37:856-868

The Escherichia coli gcvB gene encodes a small RNA transcript that is not translated in vivo. Transcription from the gcvB promoter is activated by the GcvA protein and repressed by the GcvR protein, the transcriptional regulators of the gcvTHP operon

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4f40abf1e10da23f31baeb6d023b80d
https://doi.org/10.1046/j.1365-2958.2000.02051.x

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Genetic analysis of the GcvA binding site in the gcvA control region

Autor: Alissa D. Jourdan, George V. Stauffer

Publikováno v: Microbiology. 145:2153-2162

The GcvA protein both activates and represses the gcv operon and negatively regulates its own transcription. GcvA binds to three sites in the gcv control region and to one site in the gcvA control region; each of these binding sites contains the cons

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c4a3d86fe814b08a1e4649e0056c5ba
https://doi.org/10.1099/13500872-145-8-2153

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The Cyclic AMP Receptor Protein Is Dependent on GcvA for Regulation of the gcv Operon

Autor: George V. Stauffer, Laura D. Wonderling

Publikováno v: Journal of Bacteriology. 181:1912-1919

The Escherichia coli gcv operon is transcriptionally regulated by the GcvA, GcvR, Lrp, and PurR proteins. In this study, the cyclic AMP (cAMP) receptor protein (CRP) is shown to be involved in positive regulation of the gcv operon. A crp deletion red

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f1851b799e040af4a8f073e2149e417
https://doi.org/10.1128/jb.181.6.1912-1919.1999

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