Zobrazeno 1 - 10
of 22
pro vyhledávání: '"V S Whitner"'
Publikováno v:
Clinical Chemistry. 24:916-926
The Center for Disease Control (CDC), the New York State Department of Health (NYSDH), the College of American Pathologists, and 23 manufacturers of diagnostic products participated in an interlaboratory study of aspartate aminotransferase (EC 2.6.1.
Publikováno v:
Clinical Chemistry. 26:513-519
Over 400 laboratories participated in an interlaboratory study of creatine kinase (EC 2.7.3.2) in which they were requested to analyze five lyophilized samples for total creatine kinase and creatine kinase isoenzymes and to complete a questionnaire r
Publikováno v:
Clinical Chemistry. 26:1156-1164
Publikováno v:
Clinical Chemistry. 29:793-799
We conducted a five-component, five-level response-surface experiment to optimize the pH and the concentrations of magnesium, creatine phosphate, adenosine diphosphate, and buffer in an assay for creatine kinase. Under optimal conditions, creatine ki
Publikováno v:
Clinical Chemistry. 25:659-664
Eight different pools of purified human mitochondrial and cytoplasmic isoenzymes of aspartate aminotransferase were prepared, to examine the effects of the following matrix variables: the matrix support material (bovine serum albumin and polyvinylpyr
Publikováno v:
Clinical Chemistry. 25:1691-1695
We compare a column-chromatographic method and a homogeneous immunoassay method for separately measuring the mitochondrial and cytoplasmic isoenzymes of aspartate aminotransferase. Analytical recovery for the two methods averaged 102% (SD, 2%) and 10
Autor:
Eric J. Sampson, S S McKneally, P H Duncan, D M Fast, D D Bayse, M A Baird, M L MacNeil, V S Whitner
Publikováno v:
Clinical Chemistry. 27:714-720
We describe the preparation and characterization of materials containing human pancreatic and salivary alpha-amylase (EC 3.2.1.1) and examine their relationship to endogenous amylase in human serum. Amylase was purified from human pancreas and saliva
Publikováno v:
Clinical Chemistry. 24:1805-1812
We describe a column-chromatographic method for separating the mitochondrial and cytoplasmic isoenzymes of aspartate aminotransferase in human serum. Bed height of the ion exchanger, pH, and salt concentrations in the eluting buffers are shown to be
Publikováno v:
Clinical Chemistry. 30:1322-1326
We examined the reaction surfaces around five variables (imidazole, ADP, creatine phosphate, magnesium, and pH) in the Scandinavian method for determining creatine kinase, using factorial experimentation (five level, five factor) at reaction temperat
Publikováno v:
Clinical Chemistry. 28:41-44
We examined the stability of creatine kinase (EC 2.7.3.2) isoenzyme-3 (CK-3) in lyophilized bovine albumin matrices in the presence and absence of various sulfhydryl compounds and ADP. We initially purified CK-3 from human myocardium and skeletal mus