Zobrazeno 1 - 10
of 23
pro vyhledávání: '"V N, Rybchin"'
Publikováno v:
Europe PubMed Central
N15 is the only bacteriophage of Escherichia coli known to lysogenize as a linear plasmid. Clear-plaque mutations lie in at least two regions of the 46-kb genome. We have cloned, sequenced, and characterized the primary immunity region, immB. This re
Publikováno v:
Genetika. 29(2)
The plasmids containing EcoRV fragments of N15 phage DNA and inhibiting the adsorption of T1, phi 80 and N15 phages were selected and characterized. The N15 lysogenic conversion gene (cor) was mapped in the SalI-PstI fragment of 700 bp in length whic
Publikováno v:
Genetika. 28(7)
Prophage of N15 temperate bacteriophage is stably maintained in Escherichia coli lysogens as a 46.33 kb linear plasmid. Using different transposons we obtained 18 insertion mutants of the N15 plasmid prophage. They were analysed for plaque formation
Publikováno v:
Genetika. 28(7)
Prophage of the temperate coliphage N15 is a linear plasmid with covalently closed ends. The central part of plasmid N15 genome responsible for vegetative phage growth was replaced by DNA fragments containing genes for selective markers which have un
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (5-6)
The complete structure of the pins on the ends of the linear plasmid N15 (telomers) has been defined for procaryotic organisms for the first time. The ends of the plasmid DNA contain the short nonideal inverted repeats (the size of 28 nucleotide bps)
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (5-6)
A fragment containing telRL site of bacteriophage N15 has been cloned in the vector plasmid pUC19. The nucleotide sequence of a small region from EcoRV-PstI fragment has been defined by Maxam-Gilbert technique. The analysis of the obtained sequence h
Publikováno v:
Genetika. 19(4)
A method for protoplastization of crystal- and spore-forming Bacillus thuringiensis bacterian and consequent cell wall regeneration on a solid hypertonic medium is presented. Up to 50% of the protoplasts prepared were viable and formed colonies under
Publikováno v:
Genetika. 17(11)
Hybrids lambda H lambda T80 with recombination in the region of structural genes have lambda head and phi 80 tail genes. In this paper the molecular structure of 5 independently isolated hybrids was established using restriction endonucleases. It has
Publikováno v:
Genetika. 19(4)
In the present study the possibility of obtaining interspecific bacterial hybrids by polyethylene glycol-assisted fusion of protoplasts from Bacillus thuringiensis and Bac. megaterium has been examined. Electron microscopic and genetic data allow to
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (10)
A genetic system was elaborated to study intramolecular recombination of bacteriophages lambda and phi 80. Practically, the ideal complementation of nucleotide sequences in recombining DNA molecules is required to obtain recombinants resulting from R